Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep sequencing

Here we describe a method for constructing small RNA libraries for highthroughput sequencing in which we have made a significant improvement to commonly available standard protocols. We added a locked nucleic acid (LNA) oligonucleotide—named dimer eliminator—that is complementary to the adapter-dime...

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מידע ביבליוגרפי
Main Authors:	Mitsuoki Kawano, Chika Kawazu, Marina Lizio, Hideya Kawaji, Piero Carninci, Harukazu Suzuki, Yoshihide Hayashizaki
פורמט:	Article
שפה:	English
יצא לאור:	Taylor & Francis Group 2010-10-01
סדרה:	BioTechniques
נושאים:	small RNA library adapter-dimer barcode tag high-throughput next-generation sequencer
גישה מקוונת:	https://www.future-science.com/doi/10.2144/000113516

אינטרנט

https://www.future-science.com/doi/10.2144/000113516

Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep sequencing

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