Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep sequencing

Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep sequencing

Here we describe a method for constructing small RNA libraries for highthroughput sequencing in which we have made a significant improvement to commonly available standard protocols. We added a locked nucleic acid (LNA) oligonucleotide—named dimer eliminator—that is complementary to the adapter-dime...

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Những tác giả chính: Mitsuoki Kawano, Chika Kawazu, Marina Lizio, Hideya Kawaji, Piero Carninci, Harukazu Suzuki, Yoshihide Hayashizaki
Định dạng: Bài viết
Ngôn ngữ:English
Được phát hành: Taylor & Francis Group 2010-10-01
Loạt:BioTechniques
Những chủ đề:
small RNA library
adapter-dimer
barcode tag
high-throughput
next-generation sequencer
Truy cập trực tuyến:https://www.future-science.com/doi/10.2144/000113516

Internet

https://www.future-science.com/doi/10.2144/000113516

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