Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold Stress
Sucrose metabolism plays a critical role in development, stress response, and yield formation of plants. Sucrose phosphate synthase (SPS) is the key rate-limiting enzyme in the sucrose synthesis pathway. To date, genome-wide survey and comprehensive analysis of the <i>SPS</i> gene family...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/16/12878 |
| Summary: | Sucrose metabolism plays a critical role in development, stress response, and yield formation of plants. Sucrose phosphate synthase (SPS) is the key rate-limiting enzyme in the sucrose synthesis pathway. To date, genome-wide survey and comprehensive analysis of the <i>SPS</i> gene family in soybean (<i>Glycine max</i>) have yet to be performed. In this study, seven genes encoding SPS were identified in soybean genome. The structural characteristics, phylogenetics, tissue expression patterns, and cold stress response of these <i>GmSPSs</i> were investigated. A comparative phylogenetic analysis of SPS proteins in soybean, <i>Medicago truncatula</i>, <i>Medicago sativa</i>, <i>Lotus japonicus</i>, Arabidopsis, and rice revealed four families. GmSPSs were clustered into three families from A to C, and have undergone five segmental duplication events under purifying selection. All <i>GmSPS</i> genes had various expression patterns in different tissues, and family A members <i>GmSPS13</i>/<i>17</i> were highly expressed in nodules. Remarkably, all <i>GmSPS</i> promoters contain multiple low-temperature-responsive elements such as potential binding sites of inducer of CBF expression 1 (ICE1), the central regulator in cold response. qRT-PCR proved that these <i>GmSPS</i> genes, especially <i>GmSPS8</i>/<i>18</i>, were induced by cold treatment in soybean leaves, and the expression pattern of <i>GmICE1</i> under cold treatment was similar to that of <i>GmSPS8</i>/<i>18</i>. Further transient expression analysis in <i>Nicotiana benthamiana</i> and electrophoretic mobility shift assay (EMSA) indicated that <i>GmSPS8</i> and <i>GmSPS18</i> transcriptions were directly activated by GmICE1. Taken together, our findings may aid in future efforts to clarify the potential roles of <i>GmSPS</i> genes in response to cold stress in soybean. |
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| ISSN: | 1661-6596 1422-0067 |