Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold Stress
Sucrose metabolism plays a critical role in development, stress response, and yield formation of plants. Sucrose phosphate synthase (SPS) is the key rate-limiting enzyme in the sucrose synthesis pathway. To date, genome-wide survey and comprehensive analysis of the <i>SPS</i> gene family...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/16/12878 |
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| author | Jiafang Shen Yiran Xu Songli Yuan Fuxiao Jin Yi Huang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xinan Zhou Chanjuan Zhang |
| author_facet | Jiafang Shen Yiran Xu Songli Yuan Fuxiao Jin Yi Huang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xinan Zhou Chanjuan Zhang |
| author_sort | Jiafang Shen |
| collection | DOAJ |
| description | Sucrose metabolism plays a critical role in development, stress response, and yield formation of plants. Sucrose phosphate synthase (SPS) is the key rate-limiting enzyme in the sucrose synthesis pathway. To date, genome-wide survey and comprehensive analysis of the <i>SPS</i> gene family in soybean (<i>Glycine max</i>) have yet to be performed. In this study, seven genes encoding SPS were identified in soybean genome. The structural characteristics, phylogenetics, tissue expression patterns, and cold stress response of these <i>GmSPSs</i> were investigated. A comparative phylogenetic analysis of SPS proteins in soybean, <i>Medicago truncatula</i>, <i>Medicago sativa</i>, <i>Lotus japonicus</i>, Arabidopsis, and rice revealed four families. GmSPSs were clustered into three families from A to C, and have undergone five segmental duplication events under purifying selection. All <i>GmSPS</i> genes had various expression patterns in different tissues, and family A members <i>GmSPS13</i>/<i>17</i> were highly expressed in nodules. Remarkably, all <i>GmSPS</i> promoters contain multiple low-temperature-responsive elements such as potential binding sites of inducer of CBF expression 1 (ICE1), the central regulator in cold response. qRT-PCR proved that these <i>GmSPS</i> genes, especially <i>GmSPS8</i>/<i>18</i>, were induced by cold treatment in soybean leaves, and the expression pattern of <i>GmICE1</i> under cold treatment was similar to that of <i>GmSPS8</i>/<i>18</i>. Further transient expression analysis in <i>Nicotiana benthamiana</i> and electrophoretic mobility shift assay (EMSA) indicated that <i>GmSPS8</i> and <i>GmSPS18</i> transcriptions were directly activated by GmICE1. Taken together, our findings may aid in future efforts to clarify the potential roles of <i>GmSPS</i> genes in response to cold stress in soybean. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:52:03Z |
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| spelling | doaj.art-086169cb180e48e59cfaa8300018f4bc2023-11-19T01:31:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161287810.3390/ijms241612878Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold StressJiafang Shen0Yiran Xu1Songli Yuan2Fuxiao Jin3Yi Huang4Haifeng Chen5Zhihui Shan6Zhonglu Yang7Shuilian Chen8Xinan Zhou9Chanjuan Zhang10Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaCollege of Life Sciences, Wuhan University, Wuhan 430072, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaSucrose metabolism plays a critical role in development, stress response, and yield formation of plants. Sucrose phosphate synthase (SPS) is the key rate-limiting enzyme in the sucrose synthesis pathway. To date, genome-wide survey and comprehensive analysis of the <i>SPS</i> gene family in soybean (<i>Glycine max</i>) have yet to be performed. In this study, seven genes encoding SPS were identified in soybean genome. The structural characteristics, phylogenetics, tissue expression patterns, and cold stress response of these <i>GmSPSs</i> were investigated. A comparative phylogenetic analysis of SPS proteins in soybean, <i>Medicago truncatula</i>, <i>Medicago sativa</i>, <i>Lotus japonicus</i>, Arabidopsis, and rice revealed four families. GmSPSs were clustered into three families from A to C, and have undergone five segmental duplication events under purifying selection. All <i>GmSPS</i> genes had various expression patterns in different tissues, and family A members <i>GmSPS13</i>/<i>17</i> were highly expressed in nodules. Remarkably, all <i>GmSPS</i> promoters contain multiple low-temperature-responsive elements such as potential binding sites of inducer of CBF expression 1 (ICE1), the central regulator in cold response. qRT-PCR proved that these <i>GmSPS</i> genes, especially <i>GmSPS8</i>/<i>18</i>, were induced by cold treatment in soybean leaves, and the expression pattern of <i>GmICE1</i> under cold treatment was similar to that of <i>GmSPS8</i>/<i>18</i>. Further transient expression analysis in <i>Nicotiana benthamiana</i> and electrophoretic mobility shift assay (EMSA) indicated that <i>GmSPS8</i> and <i>GmSPS18</i> transcriptions were directly activated by GmICE1. Taken together, our findings may aid in future efforts to clarify the potential roles of <i>GmSPS</i> genes in response to cold stress in soybean.https://www.mdpi.com/1422-0067/24/16/12878soybeansucrose phosphate synthasegenome-wide surveygene expressioncold stress |
| spellingShingle | Jiafang Shen Yiran Xu Songli Yuan Fuxiao Jin Yi Huang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xinan Zhou Chanjuan Zhang Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold Stress International Journal of Molecular Sciences soybean sucrose phosphate synthase genome-wide survey gene expression cold stress |
| title | Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold Stress |
| title_full | Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold Stress |
| title_fullStr | Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold Stress |
| title_full_unstemmed | Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold Stress |
| title_short | Genome-Wide Identification of <i>GmSPS</i> Gene Family in Soybean and Expression Analysis in Response to Cold Stress |
| title_sort | genome wide identification of i gmsps i gene family in soybean and expression analysis in response to cold stress |
| topic | soybean sucrose phosphate synthase genome-wide survey gene expression cold stress |
| url | https://www.mdpi.com/1422-0067/24/16/12878 |
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