Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cells
Objective(s): This study was designed to indicate whether different fractions from Artemisia biennis hydroethanolic extract could provide cytoprotection against oxidative stress and apoptosis induced by doxorubicin (DOX) in rat pheochromocytoma cell line (PC12). Material and Methods:Cell viability w...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_6935_bccfbb4325d7c6b565d254a192a65eee.pdf |
| _version_ | 1811260074200399872 |
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| author | Mahdi Mojarrab Mehran Mehrabi Farahnaz Ahmadi Leila Hosseinzadeh |
| author_facet | Mahdi Mojarrab Mehran Mehrabi Farahnaz Ahmadi Leila Hosseinzadeh |
| author_sort | Mahdi Mojarrab |
| collection | DOAJ |
| description | Objective(s): This study was designed to indicate whether different fractions from Artemisia biennis hydroethanolic extract could provide cytoprotection against oxidative stress and apoptosis induced by doxorubicin (DOX) in rat pheochromocytoma cell line (PC12). Material and Methods:Cell viability was determined by MTT assay. Also, activation of caspase-3 and superoxide dismutase were evaluated by spectrophotometry. Detection of reactive oxygen species (ROS) and measurement of mitochondrial membrane potential (MMP) were performed by flowcytometry. Results: Treatment of PC12 cells with DOX reduced viability dose dependently. For evaluation of the effect of fractions (A-G) on DOX-induced cytotoxicity, PC12 cells were pretreated for 24 hr with the A. biennis fractions and then cells were treated with DOX. The fractions C and D increased PC12 cells viability significantly compared to DOX treated cells. Moreover, pretreatment with fractions C and D for 24 hr attenuated DOX-mediated apoptosis and the anti-apoptotic action of A. biennis fractions was partially dependent on inhibition of caspase 3 activity and also increasing the mitochondrial membrane potential (MMP). Selected A. biennis fractions also suppressed the generation of ROS and increased superoxide dismutase (SOD) activity. Conclusion: Taken together our observation indicated that subtoxic concentration of aforementioned fractions of A. biennis hydroetanolic extract has protective effect against apoptosis induced by DOX in PC12 cell. The results highlighted that fractions C and D may exert cytoprotective effects through their antioxidant actions. |
| first_indexed | 2024-04-12T18:42:07Z |
| format | Article |
| id | doaj.art-0864c402fdd3462c9cc09ff86bb51a93 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T18:42:07Z |
| publishDate | 2016-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-0864c402fdd3462c9cc09ff86bb51a932022-12-22T03:20:45ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742016-05-011955035106935Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cellsMahdi Mojarrab0Mehran Mehrabi1Farahnaz Ahmadi2Leila Hosseinzadeh3Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, IranStudents Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IranPharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, IranPharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, IranObjective(s): This study was designed to indicate whether different fractions from Artemisia biennis hydroethanolic extract could provide cytoprotection against oxidative stress and apoptosis induced by doxorubicin (DOX) in rat pheochromocytoma cell line (PC12). Material and Methods:Cell viability was determined by MTT assay. Also, activation of caspase-3 and superoxide dismutase were evaluated by spectrophotometry. Detection of reactive oxygen species (ROS) and measurement of mitochondrial membrane potential (MMP) were performed by flowcytometry. Results: Treatment of PC12 cells with DOX reduced viability dose dependently. For evaluation of the effect of fractions (A-G) on DOX-induced cytotoxicity, PC12 cells were pretreated for 24 hr with the A. biennis fractions and then cells were treated with DOX. The fractions C and D increased PC12 cells viability significantly compared to DOX treated cells. Moreover, pretreatment with fractions C and D for 24 hr attenuated DOX-mediated apoptosis and the anti-apoptotic action of A. biennis fractions was partially dependent on inhibition of caspase 3 activity and also increasing the mitochondrial membrane potential (MMP). Selected A. biennis fractions also suppressed the generation of ROS and increased superoxide dismutase (SOD) activity. Conclusion: Taken together our observation indicated that subtoxic concentration of aforementioned fractions of A. biennis hydroetanolic extract has protective effect against apoptosis induced by DOX in PC12 cell. The results highlighted that fractions C and D may exert cytoprotective effects through their antioxidant actions.http://ijbms.mums.ac.ir/article_6935_bccfbb4325d7c6b565d254a192a65eee.pdfApoptosisArtemisia biennisDoxorubicinOxidative stressPC12 cell line |
| spellingShingle | Mahdi Mojarrab Mehran Mehrabi Farahnaz Ahmadi Leila Hosseinzadeh Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cells Iranian Journal of Basic Medical Sciences Apoptosis Artemisia biennis Doxorubicin Oxidative stress PC12 cell line |
| title | Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cells |
| title_full | Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cells |
| title_fullStr | Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cells |
| title_full_unstemmed | Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cells |
| title_short | Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cells |
| title_sort | protective effects of fractions from artemisia biennis hydro ethanolic extract against doxorubicin induced oxidative stress and apoptosis in pc12 cells |
| topic | Apoptosis Artemisia biennis Doxorubicin Oxidative stress PC12 cell line |
| url | http://ijbms.mums.ac.ir/article_6935_bccfbb4325d7c6b565d254a192a65eee.pdf |
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