Genetic characterization of trh positive Vibrio spp. isolated from Norway
The thermostable direct hemolysin (TDH) and/or TDH-related hemolysin (TRH) genes are carried by most virulent Vibrio parahaemolyticus serovars. In Norway, trh+ V. parahaemolyticus constitute 4.4% and 4.5 % of the total number of V. parahaemolyticus isolated from blue mussel (Mytilus edulis) and wate...
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| Format: | Article |
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Frontiers Media S.A.
2013-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2013.00107/full |
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| author | Anette eBauer Ellingsen Jaran Strand Olsen Per Einar Granum Liv Marit Rorvik Narjol eGonzález-Escalona |
| author_facet | Anette eBauer Ellingsen Jaran Strand Olsen Per Einar Granum Liv Marit Rorvik Narjol eGonzález-Escalona |
| author_sort | Anette eBauer Ellingsen |
| collection | DOAJ |
| description | The thermostable direct hemolysin (TDH) and/or TDH-related hemolysin (TRH) genes are carried by most virulent Vibrio parahaemolyticus serovars. In Norway, trh+ V. parahaemolyticus constitute 4.4% and 4.5 % of the total number of V. parahaemolyticus isolated from blue mussel (Mytilus edulis) and water, respectively. The trh gene is located in a region close to the gene cluster for urease production (ure). This region was characterized in V. parahaemolyticus strain TH3996 and it was found that a nickel transport operon (nik) was located between the first gene (ureR) and the rest of the ure cluster genes. The organization of the trh-ureR-nik-ure gene cluster in the Norwegian trh+ isolates was unknown. In this study, we explore the gene organization within the trh-ureR-nik-ure cluster for these isolates. PCR analyses revealed that the genes within the trh-ureR-nik-ure gene cluster of Norwegian trh+ isolates were organized in a similar fashion as reported previously for TH33996. Additionally, the phylogenetic relationship among these trh+ isolates was investigated using Multilocus Sequence Typing (MLST). Analysis by MLST or ureR-trh sequences generated two different phylogenetic trees for the same strains analyzed, suggesting that ureR-trh genes have been acquired at different times in Norwegian V. parahaemolyticus isolates. MLST results revealed that some pathogenic and non-pathogenic V. parahaemolyticus isolates in Norway appear to be highly genetically related. |
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| format | Article |
| id | doaj.art-0865a4e3e1a9469fb590e7107017eec6 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-20T20:52:39Z |
| publishDate | 2013-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-0865a4e3e1a9469fb590e7107017eec62022-12-21T19:26:53ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882013-12-01310.3389/fcimb.2013.0010766601Genetic characterization of trh positive Vibrio spp. isolated from NorwayAnette eBauer Ellingsen0Jaran Strand Olsen1Per Einar Granum2Liv Marit Rorvik3Narjol eGonzález-Escalona4Norwegian School of Veterinary ScienceNorwegian Defence Research EstablishmentNorwegian School of Veterinary ScienceNorwegian School of Veterinary ScienceFood and Drug AdministrationThe thermostable direct hemolysin (TDH) and/or TDH-related hemolysin (TRH) genes are carried by most virulent Vibrio parahaemolyticus serovars. In Norway, trh+ V. parahaemolyticus constitute 4.4% and 4.5 % of the total number of V. parahaemolyticus isolated from blue mussel (Mytilus edulis) and water, respectively. The trh gene is located in a region close to the gene cluster for urease production (ure). This region was characterized in V. parahaemolyticus strain TH3996 and it was found that a nickel transport operon (nik) was located between the first gene (ureR) and the rest of the ure cluster genes. The organization of the trh-ureR-nik-ure gene cluster in the Norwegian trh+ isolates was unknown. In this study, we explore the gene organization within the trh-ureR-nik-ure cluster for these isolates. PCR analyses revealed that the genes within the trh-ureR-nik-ure gene cluster of Norwegian trh+ isolates were organized in a similar fashion as reported previously for TH33996. Additionally, the phylogenetic relationship among these trh+ isolates was investigated using Multilocus Sequence Typing (MLST). Analysis by MLST or ureR-trh sequences generated two different phylogenetic trees for the same strains analyzed, suggesting that ureR-trh genes have been acquired at different times in Norwegian V. parahaemolyticus isolates. MLST results revealed that some pathogenic and non-pathogenic V. parahaemolyticus isolates in Norway appear to be highly genetically related.http://journal.frontiersin.org/Journal/10.3389/fcimb.2013.00107/fullUreaseVibriomlsttrhtdhV. parahaemolyticus |
| spellingShingle | Anette eBauer Ellingsen Jaran Strand Olsen Per Einar Granum Liv Marit Rorvik Narjol eGonzález-Escalona Genetic characterization of trh positive Vibrio spp. isolated from Norway Frontiers in Cellular and Infection Microbiology Urease Vibrio mlst trh tdh V. parahaemolyticus |
| title | Genetic characterization of trh positive Vibrio spp. isolated from Norway |
| title_full | Genetic characterization of trh positive Vibrio spp. isolated from Norway |
| title_fullStr | Genetic characterization of trh positive Vibrio spp. isolated from Norway |
| title_full_unstemmed | Genetic characterization of trh positive Vibrio spp. isolated from Norway |
| title_short | Genetic characterization of trh positive Vibrio spp. isolated from Norway |
| title_sort | genetic characterization of trh positive vibrio spp isolated from norway |
| topic | Urease Vibrio mlst trh tdh V. parahaemolyticus |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2013.00107/full |
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