Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala
Abstract We recently reported that a neuronal population in the claustrum (CLA) identified under exposure to psychological stressors plays a key role in stress response processing. Upon stress exposure, the main inputs to the CLA come from the basolateral amygdala (BLA); however, the upstream brain...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13041-022-00987-8 |
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| author | Masato Tanuma Misaki Niu Jin Ohkubo Hiroki Ueno Yuka Nakai Yoshihisa Yokoyama Kaoru Seiriki Hitoshi Hashimoto Atsushi Kasai |
| author_facet | Masato Tanuma Misaki Niu Jin Ohkubo Hiroki Ueno Yuka Nakai Yoshihisa Yokoyama Kaoru Seiriki Hitoshi Hashimoto Atsushi Kasai |
| author_sort | Masato Tanuma |
| collection | DOAJ |
| description | Abstract We recently reported that a neuronal population in the claustrum (CLA) identified under exposure to psychological stressors plays a key role in stress response processing. Upon stress exposure, the main inputs to the CLA come from the basolateral amygdala (BLA); however, the upstream brain regions that potentially regulate both the CLA and BLA during stressful experiences remain unclear. Here by combining activity-dependent viral retrograde labeling with whole brain imaging, we analyzed neurons projecting to the CLA and BLA activated by exposure to social defeat stress. The labeled CLA projecting neurons were mostly ipsilateral, excluding the prefrontal cortices, which had a distinctly labeled population in the contralateral hemisphere. Similarly, the labeled BLA projecting neurons were predominantly ipsilateral, aside from the BLA in the opposite hemisphere, which also had a notably labeled population. Moreover, we found co-labeled double-projecting single neurons in multiple brain regions such as the ipsilateral ectorhinal/perirhinal cortex, entorhinal cortex, and the contralateral BLA. These results suggest that CLA and BLA receive inputs from neuron collaterals in various brain regions during stress, which may regulate the CLA and BLA forming in a stress response circuitry. |
| first_indexed | 2024-04-11T05:02:40Z |
| format | Article |
| id | doaj.art-086aa11252254f0981c6cba6fe5ff931 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-04-11T05:02:40Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-086aa11252254f0981c6cba6fe5ff9312022-12-25T12:34:25ZengBMCMolecular Brain1756-66062022-12-0115111110.1186/s13041-022-00987-8Acute social defeat stress activated neurons project to the claustrum and basolateral amygdalaMasato Tanuma0Misaki Niu1Jin Ohkubo2Hiroki Ueno3Yuka Nakai4Yoshihisa Yokoyama5Kaoru Seiriki6Hitoshi Hashimoto7Atsushi Kasai8Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka UniversityAbstract We recently reported that a neuronal population in the claustrum (CLA) identified under exposure to psychological stressors plays a key role in stress response processing. Upon stress exposure, the main inputs to the CLA come from the basolateral amygdala (BLA); however, the upstream brain regions that potentially regulate both the CLA and BLA during stressful experiences remain unclear. Here by combining activity-dependent viral retrograde labeling with whole brain imaging, we analyzed neurons projecting to the CLA and BLA activated by exposure to social defeat stress. The labeled CLA projecting neurons were mostly ipsilateral, excluding the prefrontal cortices, which had a distinctly labeled population in the contralateral hemisphere. Similarly, the labeled BLA projecting neurons were predominantly ipsilateral, aside from the BLA in the opposite hemisphere, which also had a notably labeled population. Moreover, we found co-labeled double-projecting single neurons in multiple brain regions such as the ipsilateral ectorhinal/perirhinal cortex, entorhinal cortex, and the contralateral BLA. These results suggest that CLA and BLA receive inputs from neuron collaterals in various brain regions during stress, which may regulate the CLA and BLA forming in a stress response circuitry.https://doi.org/10.1186/s13041-022-00987-8ClaustrumBasolateral amygdalaActivity-dependent labelingRetrograde tracingCollateral projectionsStress |
| spellingShingle | Masato Tanuma Misaki Niu Jin Ohkubo Hiroki Ueno Yuka Nakai Yoshihisa Yokoyama Kaoru Seiriki Hitoshi Hashimoto Atsushi Kasai Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala Molecular Brain Claustrum Basolateral amygdala Activity-dependent labeling Retrograde tracing Collateral projections Stress |
| title | Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala |
| title_full | Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala |
| title_fullStr | Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala |
| title_full_unstemmed | Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala |
| title_short | Acute social defeat stress activated neurons project to the claustrum and basolateral amygdala |
| title_sort | acute social defeat stress activated neurons project to the claustrum and basolateral amygdala |
| topic | Claustrum Basolateral amygdala Activity-dependent labeling Retrograde tracing Collateral projections Stress |
| url | https://doi.org/10.1186/s13041-022-00987-8 |
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