Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae
The main goal of our work is the presentation and analysis of incidence of porcine respiratory disease complex (PRDC) regarding bacterial agents in the territory of northern districts of Slovakia. Mycoplasma hyopneumoniae and other secondary bacterial causative pathogens of PRDC comprised 75.2% of a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2004-02-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200402-0001_diagnostics-of-main-bacterial-agents-of-porcine-respiratory-diseases-complex-prdc-using-pcr-detection-of-myco.php |
| _version_ | 1797897705669787648 |
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| author | I. Holko J. Urbanova Tholkova V. Kmet |
| author_facet | I. Holko J. Urbanova Tholkova V. Kmet |
| author_sort | I. Holko |
| collection | DOAJ |
| description | The main goal of our work is the presentation and analysis of incidence of porcine respiratory disease complex (PRDC) regarding bacterial agents in the territory of northern districts of Slovakia. Mycoplasma hyopneumoniae and other secondary bacterial causative pathogens of PRDC comprised 75.2% of all cases (98) with clinical signs of respiratory infections that we examined in the course of one year. We present also one of possibilities to the solution of problematic detection of M. hyopneumoniae which is, like the whole rank of mycoplasmas, very difficult to cultivate. This problem was solved by using the PCR method with the direct isolation of M. hyopneumoniae from lungs tissue. In antibiotic sensitivity testing of Pasteurella multocida and Actinobacillus pleuropneumoniae resulted enrofloxacin as the most effective antibiotics in the therapy of PRDC regarding bacterial agents.in above mentioned territory. |
| first_indexed | 2024-04-10T08:02:34Z |
| format | Article |
| id | doaj.art-086b1dd914ff47b0b41d31393ebfc332 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:34Z |
| publishDate | 2004-02-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-086b1dd914ff47b0b41d31393ebfc3322023-02-23T03:49:09ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922004-02-01492354110.17221/5672-VETMEDvet-200402-0001Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniaeI. Holko0J. Urbanova1Tholkova2V. Kmet3State Veterinary Institute, Prague, Czech RepublicState Veterinary Institute, Prague, Czech RepublicState Veterinary Institute, Prague, Czech RepublicState Veterinary Institute, Prague, Czech RepublicThe main goal of our work is the presentation and analysis of incidence of porcine respiratory disease complex (PRDC) regarding bacterial agents in the territory of northern districts of Slovakia. Mycoplasma hyopneumoniae and other secondary bacterial causative pathogens of PRDC comprised 75.2% of all cases (98) with clinical signs of respiratory infections that we examined in the course of one year. We present also one of possibilities to the solution of problematic detection of M. hyopneumoniae which is, like the whole rank of mycoplasmas, very difficult to cultivate. This problem was solved by using the PCR method with the direct isolation of M. hyopneumoniae from lungs tissue. In antibiotic sensitivity testing of Pasteurella multocida and Actinobacillus pleuropneumoniae resulted enrofloxacin as the most effective antibiotics in the therapy of PRDC regarding bacterial agents.in above mentioned territory.https://vetmed.agriculturejournals.cz/artkey/vet-200402-0001_diagnostics-of-main-bacterial-agents-of-porcine-respiratory-diseases-complex-prdc-using-pcr-detection-of-myco.phppigspasteurella multocidaactinobacillus pleuropneumoniaemycoplasma hyopneumoniaeantibiotic sensitivitypcr |
| spellingShingle | I. Holko J. Urbanova Tholkova V. Kmet Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae Veterinární Medicína pigs pasteurella multocida actinobacillus pleuropneumoniae mycoplasma hyopneumoniae antibiotic sensitivity pcr |
| title | Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae |
| title_full | Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae |
| title_fullStr | Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae |
| title_full_unstemmed | Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae |
| title_short | Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae |
| title_sort | diagnostics of main bacterial agents of porcine respiratory diseases complex prdc using pcr detection of mycoplasma hyopneumoniae |
| topic | pigs pasteurella multocida actinobacillus pleuropneumoniae mycoplasma hyopneumoniae antibiotic sensitivity pcr |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200402-0001_diagnostics-of-main-bacterial-agents-of-porcine-respiratory-diseases-complex-prdc-using-pcr-detection-of-myco.php |
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