Rotavirus Quantification in Children with Acute Gastroenteritis
Introduction: Most intractable diarrheas are treated with antibiotics, irrespective of the causative agent. This study aimed to quantify rotavirus (RV) with TaqMan real-time PCR in fecal samples of children with acute gastroenteritis (AGE) in accordance with the program of reduction of drug resistan...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Karger Publishers
2022-10-01
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| Series: | Intervirology |
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| Online Access: | https://www.karger.com/Article/FullText/526839 |
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| author | Ilaria Galliano Valentina Daprà Maddalena Dini Martina Gavatorta Allegra Sardo Giada Lo Curcio Massimiliano Bergallo |
| author_facet | Ilaria Galliano Valentina Daprà Maddalena Dini Martina Gavatorta Allegra Sardo Giada Lo Curcio Massimiliano Bergallo |
| author_sort | Ilaria Galliano |
| collection | DOAJ |
| description | Introduction: Most intractable diarrheas are treated with antibiotics, irrespective of the causative agent. This study aimed to quantify rotavirus (RV) with TaqMan real-time PCR in fecal samples of children with acute gastroenteritis (AGE) in accordance with the program of reduction of drug resistance and use of antibiotics. Methods: A total of 190 fecal specimens were collected from under-5-year-old children with AGE in pediatric Hospital Regina Margherita of Turin in Italy in 2017. A total of 38 out of 67 (56.7%) episodes of AGE were associated with RV genomic detection with a multiplex commercial kit. Results: All fecal specimens were tested for the presence of RV and other GE viruses. The most commonly detected virus was norovirus (41%), astrovirus (15.8%), human bocavirus (8.9%), sapovirus (7.9%), human parechovirus (5.8%), rhinovirus (4.2%), and adenovirus (1%). In 66 out of 190 (34.7%), RV was detectable with the median viral load 7.2E + 11 ± 60E + 11 genomes/mg fecal specimens. Discussion/Conclusions: Our results showed that RV was present in around 34.7% of children hospitalized for AGE, a rate similar to those reported in previous studies conducted elsewhere which were in the range of 33–75%. Our protocols are able to quantify the absolute number of viral particle/mg of feces. The clinical utility of quantitative molecular assays, such as RV viral load, will be markedly improved. |
| first_indexed | 2024-04-13T21:58:52Z |
| format | Article |
| id | doaj.art-086b82c8cce04247b4b9c9e3a1712947 |
| institution | Directory Open Access Journal |
| issn | 0300-5526 1423-0100 |
| language | English |
| last_indexed | 2024-04-13T21:58:52Z |
| publishDate | 2022-10-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Intervirology |
| spelling | doaj.art-086b82c8cce04247b4b9c9e3a17129472022-12-22T02:28:10ZengKarger PublishersIntervirology0300-55261423-01002022-10-0110.1159/000526839526839Rotavirus Quantification in Children with Acute GastroenteritisIlaria Galliano0Valentina Daprà1Maddalena Dini2Martina Gavatorta3Allegra Sardo4Giada Lo Curcio5Massimiliano Bergallo6https://orcid.org/0000-0002-9291-1332Department of Public Health and Pediatric Sciences, Pediatrics Laboratory, University of Turin, Turin, ItalyDepartment of Public Health and Pediatric Sciences, Pediatrics Laboratory, University of Turin, Turin, ItalyDepartment of Public Health and Pediatric Sciences, Pediatrics Laboratory, University of Turin, Turin, ItalyDepartment of Public Health and Pediatric Sciences, Pediatrics Laboratory, University of Turin, Turin, ItalyDepartment of Public Health and Pediatric Sciences, Pediatrics Laboratory, University of Turin, Turin, ItalyDepartment of Public Health and Pediatric Sciences, Pediatrics Laboratory, University of Turin, Turin, ItalyDepartment of Public Health and Pediatric Sciences, Pediatrics Laboratory, University of Turin, Turin, ItalyIntroduction: Most intractable diarrheas are treated with antibiotics, irrespective of the causative agent. This study aimed to quantify rotavirus (RV) with TaqMan real-time PCR in fecal samples of children with acute gastroenteritis (AGE) in accordance with the program of reduction of drug resistance and use of antibiotics. Methods: A total of 190 fecal specimens were collected from under-5-year-old children with AGE in pediatric Hospital Regina Margherita of Turin in Italy in 2017. A total of 38 out of 67 (56.7%) episodes of AGE were associated with RV genomic detection with a multiplex commercial kit. Results: All fecal specimens were tested for the presence of RV and other GE viruses. The most commonly detected virus was norovirus (41%), astrovirus (15.8%), human bocavirus (8.9%), sapovirus (7.9%), human parechovirus (5.8%), rhinovirus (4.2%), and adenovirus (1%). In 66 out of 190 (34.7%), RV was detectable with the median viral load 7.2E + 11 ± 60E + 11 genomes/mg fecal specimens. Discussion/Conclusions: Our results showed that RV was present in around 34.7% of children hospitalized for AGE, a rate similar to those reported in previous studies conducted elsewhere which were in the range of 33–75%. Our protocols are able to quantify the absolute number of viral particle/mg of feces. The clinical utility of quantitative molecular assays, such as RV viral load, will be markedly improved.https://www.karger.com/Article/FullText/526839gastroenteritisreal-time pcrviral loadrotavirus |
| spellingShingle | Ilaria Galliano Valentina Daprà Maddalena Dini Martina Gavatorta Allegra Sardo Giada Lo Curcio Massimiliano Bergallo Rotavirus Quantification in Children with Acute Gastroenteritis Intervirology gastroenteritis real-time pcr viral load rotavirus |
| title | Rotavirus Quantification in Children with Acute Gastroenteritis |
| title_full | Rotavirus Quantification in Children with Acute Gastroenteritis |
| title_fullStr | Rotavirus Quantification in Children with Acute Gastroenteritis |
| title_full_unstemmed | Rotavirus Quantification in Children with Acute Gastroenteritis |
| title_short | Rotavirus Quantification in Children with Acute Gastroenteritis |
| title_sort | rotavirus quantification in children with acute gastroenteritis |
| topic | gastroenteritis real-time pcr viral load rotavirus |
| url | https://www.karger.com/Article/FullText/526839 |
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