Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in shahrekord, Iran
Introduction : Staphylococcus aureus is considered as one of pathogenic agents in humans, that engages different body parts including respiratory system and causes to spend lots of costs and extending patient’s treatment period. This study which is performed to separate and investigate the pattern o...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2014-07-01
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| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-306-1&slc_lang=en&sid=1 |
| _version_ | 1818068438113845248 |
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| author | Maryam Reisi Elahe Tajbakhsh Hassan Momtaz |
| author_facet | Maryam Reisi Elahe Tajbakhsh Hassan Momtaz |
| author_sort | Maryam Reisi |
| collection | DOAJ |
| description | Introduction : Staphylococcus aureus is considered as one of pathogenic agents in humans, that engages different body parts including respiratory system and causes to spend lots of costs and extending patient’s treatment period. This study which is performed to separate and investigate the pattern of antibiotic resistance in Staphylococcus aureus isolates from upper respiratory system infections in Shahrekord. Materials and methods: This study was done by sectional-descriptive method On 200 suspicious persons to the upper respiratory system infections who were referred to the Imam Ali clinic in Shahrekord in 2012. After isolation of Staphylococcus aureus from cultured nose discharges, antibiotic resistance genes were identified by polymerase chain reaction (PCR) by using defined primer pairs . Results : Among 200 investigated samples in 60 cases (30%) Staphylococcus aureus infection (by culturing and PCR method) was determined. Isolates showed the lowest amount of antibiotic resistance to vancomycin (0.5%) and the highest amount of resistance to the penicillin G and cefotaxime (100%). mecA gene (encoding methicillin resistance) with frequency of 85.18% and aacA-D gene (encoding resistance to aminoglycosides) with frequency of 28.33% showed the highest and lowest frequency of antibiotic resistance genes coding in Staphylococcus aureus isolates respectively . Discussion and conclusion : Notable prevalence of resistant Staphylococcus aureus isolates in community acquired respiratory infections, recommend continuous control necessity to impede the spreading of these bacteria and their infections. |
| first_indexed | 2024-12-10T15:39:34Z |
| format | Article |
| id | doaj.art-086c2133e5094426a3e99664be544abb |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-10T15:39:34Z |
| publishDate | 2014-07-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-086c2133e5094426a3e99664be544abb2022-12-22T01:43:08ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812014-07-0131097106Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in shahrekord, IranMaryam Reisi0Elahe Tajbakhsh1Hassan Momtaz2 Islamic Azad University, Shahrekord, Iran Islamic Azad University, Shahrekord, Iran Islamic Azad University, Shahrekord, Iran Introduction : Staphylococcus aureus is considered as one of pathogenic agents in humans, that engages different body parts including respiratory system and causes to spend lots of costs and extending patient’s treatment period. This study which is performed to separate and investigate the pattern of antibiotic resistance in Staphylococcus aureus isolates from upper respiratory system infections in Shahrekord. Materials and methods: This study was done by sectional-descriptive method On 200 suspicious persons to the upper respiratory system infections who were referred to the Imam Ali clinic in Shahrekord in 2012. After isolation of Staphylococcus aureus from cultured nose discharges, antibiotic resistance genes were identified by polymerase chain reaction (PCR) by using defined primer pairs . Results : Among 200 investigated samples in 60 cases (30%) Staphylococcus aureus infection (by culturing and PCR method) was determined. Isolates showed the lowest amount of antibiotic resistance to vancomycin (0.5%) and the highest amount of resistance to the penicillin G and cefotaxime (100%). mecA gene (encoding methicillin resistance) with frequency of 85.18% and aacA-D gene (encoding resistance to aminoglycosides) with frequency of 28.33% showed the highest and lowest frequency of antibiotic resistance genes coding in Staphylococcus aureus isolates respectively . Discussion and conclusion : Notable prevalence of resistant Staphylococcus aureus isolates in community acquired respiratory infections, recommend continuous control necessity to impede the spreading of these bacteria and their infections.http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-306-1&slc_lang=en&sid=1Antibiotic resistance genes Staphylococcus aureus Respiratory system infection |
| spellingShingle | Maryam Reisi Elahe Tajbakhsh Hassan Momtaz Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in shahrekord, Iran Biological Journal of Microorganism Antibiotic resistance genes Staphylococcus aureus Respiratory system infection |
| title | Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in shahrekord, Iran |
| title_full | Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in shahrekord, Iran |
| title_fullStr | Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in shahrekord, Iran |
| title_full_unstemmed | Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in shahrekord, Iran |
| title_short | Isolation and identification of antibiotic resistance genes in Staphylococcus aureus isolates from respiratory system infections in shahrekord, Iran |
| title_sort | isolation and identification of antibiotic resistance genes in staphylococcus aureus isolates from respiratory system infections in shahrekord iran |
| topic | Antibiotic resistance genes Staphylococcus aureus Respiratory system infection |
| url | http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-306-1&slc_lang=en&sid=1 |
| work_keys_str_mv | AT maryamreisi isolationandidentificationofantibioticresistancegenesinstaphylococcusaureusisolatesfromrespiratorysysteminfectionsinshahrekordiran AT elahetajbakhsh isolationandidentificationofantibioticresistancegenesinstaphylococcusaureusisolatesfromrespiratorysysteminfectionsinshahrekordiran AT hassanmomtaz isolationandidentificationofantibioticresistancegenesinstaphylococcusaureusisolatesfromrespiratorysysteminfectionsinshahrekordiran |