Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate
Abstract Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we e...
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| Format: | Article |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-05424-8 |
| _version_ | 1818750747913748480 |
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| author | Hae-Chang Jung Seong Hyuk Lee Sung-Mok Lee Young Jun An Jung-Hyun Lee Hyun Sook Lee Sung Gyun Kang |
| author_facet | Hae-Chang Jung Seong Hyuk Lee Sung-Mok Lee Young Jun An Jung-Hyun Lee Hyun Sook Lee Sung Gyun Kang |
| author_sort | Hae-Chang Jung |
| collection | DOAJ |
| description | Abstract Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecular changes. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF-156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genome sequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found among the genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formate transporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain, conferred increase in formate consumption and H2 production. Another adaptive passage, carried out by culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth. |
| first_indexed | 2024-12-18T04:24:36Z |
| format | Article |
| id | doaj.art-086cad0d667a456e8904231d11746938 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:24:36Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-086cad0d667a456e8904231d117469382022-12-21T21:21:08ZengNature PortfolioScientific Reports2045-23222017-07-01711910.1038/s41598-017-05424-8Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formateHae-Chang Jung0Seong Hyuk Lee1Sung-Mok Lee2Young Jun An3Jung-Hyun Lee4Hyun Sook Lee5Sung Gyun Kang6Korea Institute of Ocean Science and TechnologyKorea Institute of Ocean Science and TechnologyKorea Institute of Ocean Science and TechnologyKorea Institute of Ocean Science and TechnologyKorea Institute of Ocean Science and TechnologyKorea Institute of Ocean Science and TechnologyKorea Institute of Ocean Science and TechnologyAbstract Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecular changes. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF-156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genome sequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found among the genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formate transporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain, conferred increase in formate consumption and H2 production. Another adaptive passage, carried out by culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth.https://doi.org/10.1038/s41598-017-05424-8 |
| spellingShingle | Hae-Chang Jung Seong Hyuk Lee Sung-Mok Lee Young Jun An Jung-Hyun Lee Hyun Sook Lee Sung Gyun Kang Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate Scientific Reports |
| title | Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate |
| title_full | Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate |
| title_fullStr | Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate |
| title_full_unstemmed | Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate |
| title_short | Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate |
| title_sort | adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate |
| url | https://doi.org/10.1038/s41598-017-05424-8 |
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