Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, Iran
Background: Echinococcosis is one of the most important helminthic zoonotic diseases in Iran. Intestinal Scraping Technique (IST), the traditional method for diagnosis of the infection in definitive hosts, has many disadvantages including low susceptibility and being expensive, hazardous and labo...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2009-03-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/3218 |
| _version_ | 1818567701395668992 |
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| author | M Zare-Bidaki I Mobedi SR Naddaf EB Kia M Mahmoudi N Piazak H Nekouie S Sadeghieh Ahari Sh Habibzadeh M Siavashi |
| author_facet | M Zare-Bidaki I Mobedi SR Naddaf EB Kia M Mahmoudi N Piazak H Nekouie S Sadeghieh Ahari Sh Habibzadeh M Siavashi |
| author_sort | M Zare-Bidaki |
| collection | DOAJ |
| description | Background: Echinococcosis is one of the most important helminthic zoonotic diseases in Iran. Intestinal Scraping Technique (IST), the traditional method for diagnosis of the infection in definitive hosts, has many disadvantages including low susceptibility and being expensive, hazardous and laborious. Detection of coproantigens in fecal samples by enzyme-linked immunosorbent assay (CA-ELISA) is known as a useful tool for intravital mass-screening of definitive host populations. This study was performed to determine the prevalence of Echinococcus spp. infection among canids in Moghan plain, the only area in Iran known to be endemic for E. multilocularis.
Methods: One hundred thirty eight fecal samples were collected from red foxes and domestic dogs in three counties of Moghan plain namely Pars Abad, Bileh Savar and Germi. The samples were examined using an ELISA, designed for the detection of Echinococcus-specific coproantigen and the formalin-ether concentration method as well.
Results: Totally, out of 138 fecal samples, 27 (21.6%) turned positive for Echinococcus. Coproantigen was detected in 16.7% and 27.1% of red foxes and domestic dogs, respectively. Formalin-ether concentration method revealed that 43 (31.2%) of samples harbored at least one parasitic helminth, but Taenia eggs were detected only in 3 fecal samples. Since coproantigen presence reflects current intestinal infection with adult worms, CA-ELISA can be regarded as one of the most useful immunological tools for diagnosis of Echinococcus infection. Besides, the high susceptibility, low cost and rapidity |
| first_indexed | 2024-12-14T06:26:46Z |
| format | Article |
| id | doaj.art-086dfd9b2ca3418499e5ec222265ce2f |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-14T06:26:46Z |
| publishDate | 2009-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-086dfd9b2ca3418499e5ec222265ce2f2022-12-21T23:13:39ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932009-03-01381Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, IranM Zare-Bidaki0I Mobedi1SR Naddaf 2EB Kia 3M Mahmoudi 4N Piazak5H Nekouie 6S Sadeghieh Ahari 7Sh Habibzadeh 8M Siavashi 9Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical ScDept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical ScDept. of Medical Parasitology, Pasteur Institute of Iran, Tehran, IranDept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical ScDept.of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical SciencDept. of Medical Parasitology, Pasteur Institute of Iran, Tehran, IranDept. of Medical Parasitology, Pasteur Institute of Iran, Tehran, IranDept. of Pediatrics and Social Medicine, School of Medicine, Ardebil University of Medical Sciences,Dept.of Infection, School of Medicine, Ardebil University of Medical Sciences, IranDept. of Medical Parasitology, Pasteur Institute of Iran, Tehran, IranBackground: Echinococcosis is one of the most important helminthic zoonotic diseases in Iran. Intestinal Scraping Technique (IST), the traditional method for diagnosis of the infection in definitive hosts, has many disadvantages including low susceptibility and being expensive, hazardous and laborious. Detection of coproantigens in fecal samples by enzyme-linked immunosorbent assay (CA-ELISA) is known as a useful tool for intravital mass-screening of definitive host populations. This study was performed to determine the prevalence of Echinococcus spp. infection among canids in Moghan plain, the only area in Iran known to be endemic for E. multilocularis. Methods: One hundred thirty eight fecal samples were collected from red foxes and domestic dogs in three counties of Moghan plain namely Pars Abad, Bileh Savar and Germi. The samples were examined using an ELISA, designed for the detection of Echinococcus-specific coproantigen and the formalin-ether concentration method as well. Results: Totally, out of 138 fecal samples, 27 (21.6%) turned positive for Echinococcus. Coproantigen was detected in 16.7% and 27.1% of red foxes and domestic dogs, respectively. Formalin-ether concentration method revealed that 43 (31.2%) of samples harbored at least one parasitic helminth, but Taenia eggs were detected only in 3 fecal samples. Since coproantigen presence reflects current intestinal infection with adult worms, CA-ELISA can be regarded as one of the most useful immunological tools for diagnosis of Echinococcus infection. Besides, the high susceptibility, low cost and rapidityhttps://ijph.tums.ac.ir/index.php/ijph/article/view/3218CoproantigenEchinococcosisEchinococcusCA-ELISAIran |
| spellingShingle | M Zare-Bidaki I Mobedi SR Naddaf EB Kia M Mahmoudi N Piazak H Nekouie S Sadeghieh Ahari Sh Habibzadeh M Siavashi Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, Iran Iranian Journal of Public Health Coproantigen Echinococcosis Echinococcus CA-ELISA Iran |
| title | Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, Iran |
| title_full | Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, Iran |
| title_fullStr | Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, Iran |
| title_full_unstemmed | Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, Iran |
| title_short | Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, Iran |
| title_sort | prevalence of echinococcus spp infection using coproantigen elisa among canids of moghan plain iran |
| topic | Coproantigen Echinococcosis Echinococcus CA-ELISA Iran |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/3218 |
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