BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive Sprouts
Given the importance of olive sprouts, it is crucial to explore their potential as an innovative source of bioactive and nutritive compounds through research. Here, we aimed at investigating the potential role of benzylaminopurine (BAP) in improving the tissue chemical composition and bioactivity of...
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| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/9/1055 |
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| author | Samy Selim Ahlem Zrig Mha Albqmi Mohammad M. Al-Sanea Taghreed S. Alnusaire Mohammed S. Almuhayawi Soad K. Al Jaouni Shaimaa Hussein Mona Warrad Hamada AbdElgawad |
| author_facet | Samy Selim Ahlem Zrig Mha Albqmi Mohammad M. Al-Sanea Taghreed S. Alnusaire Mohammed S. Almuhayawi Soad K. Al Jaouni Shaimaa Hussein Mona Warrad Hamada AbdElgawad |
| author_sort | Samy Selim |
| collection | DOAJ |
| description | Given the importance of olive sprouts, it is crucial to explore their potential as an innovative source of bioactive and nutritive compounds through research. Here, we aimed at investigating the potential role of benzylaminopurine (BAP) in improving the tissue chemical composition and bioactivity of olive sprouts. To this end, seeds of two olive varieties (<i>Olea europaea</i> L. vr. Kroniaki and Coratina) were primed with BAP at 25 µM. A substantial enhancement was observed in biomass accumulation by 35% and 30% in Kroniaki and Coratina varieties, respectively. Likewise, the photosynthetic pigments (total chlorophyll, α- and β-carotene, lutein and β-cryptoxanthin) in both varieties were increased, mainly in Coratina. At primary metabolic level, BAP priming improved sprout lipid composition, particularly in Coratina variety. At antioxidant level, BAP priming improved lipid antioxidants (α-, β- and γ-tocopherols) and water-soluble antioxidants (phenols, flavonoids, ascorbate, glutathione and anthocyanins) in both olive varieties. At the anthocyanins level, their precursors (phenylalanine, cinnamic acid, coumaric acid and naringenin) and key biosynthetic enzyme activity (phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), coenzyme A ligase (4CL) and cinnamate 4-hydroxylase (C4H)) were improved in olive varieties, but to a greater extent in Coratina variety. Overall, the sprouts of BAP-primed olive seeds could potentially enhance their nutritional value, suggesting that the sprouts of BAP-primed olive seeds can be used as a food ingredient and additives. |
| first_indexed | 2024-03-10T22:40:52Z |
| format | Article |
| id | doaj.art-086f0c7725e64383a0af2e7366faadff |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T22:40:52Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-086f0c7725e64383a0af2e7366faadff2023-11-19T10:59:34ZengMDPI AGHorticulturae2311-75242023-09-0199105510.3390/horticulturae9091055BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive SproutsSamy Selim0Ahlem Zrig1Mha Albqmi2Mohammad M. Al-Sanea3Taghreed S. Alnusaire4Mohammed S. Almuhayawi5Soad K. Al Jaouni6Shaimaa Hussein7Mona Warrad8Hamada AbdElgawad9Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72388, Saudi ArabiaHigher Institute of Preparatory Studies in Biology and Geology, University of Carthage, Tunis 1073, TunisiaOlive Research Center, Jouf University, Sakaka 72388, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka 72388, Saudi ArabiaDepartment of Biology, College of Science, Jouf University, Sakaka 72388, Saudi ArabiaDepartment of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz University, Jeddah 25729, Saudi ArabiaDepartment of Hematology and Oncology, Yousef Abdulatif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah 25729, Saudi ArabiaDepartment of Pharmacology, College of Pharmacy, Jouf University, Sakaka 72388, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences at Al-Quriat, Jouf University, Al Qurayyat 77425, Saudi ArabiaDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef 62521, EgyptGiven the importance of olive sprouts, it is crucial to explore their potential as an innovative source of bioactive and nutritive compounds through research. Here, we aimed at investigating the potential role of benzylaminopurine (BAP) in improving the tissue chemical composition and bioactivity of olive sprouts. To this end, seeds of two olive varieties (<i>Olea europaea</i> L. vr. Kroniaki and Coratina) were primed with BAP at 25 µM. A substantial enhancement was observed in biomass accumulation by 35% and 30% in Kroniaki and Coratina varieties, respectively. Likewise, the photosynthetic pigments (total chlorophyll, α- and β-carotene, lutein and β-cryptoxanthin) in both varieties were increased, mainly in Coratina. At primary metabolic level, BAP priming improved sprout lipid composition, particularly in Coratina variety. At antioxidant level, BAP priming improved lipid antioxidants (α-, β- and γ-tocopherols) and water-soluble antioxidants (phenols, flavonoids, ascorbate, glutathione and anthocyanins) in both olive varieties. At the anthocyanins level, their precursors (phenylalanine, cinnamic acid, coumaric acid and naringenin) and key biosynthetic enzyme activity (phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), coenzyme A ligase (4CL) and cinnamate 4-hydroxylase (C4H)) were improved in olive varieties, but to a greater extent in Coratina variety. Overall, the sprouts of BAP-primed olive seeds could potentially enhance their nutritional value, suggesting that the sprouts of BAP-primed olive seeds can be used as a food ingredient and additives.https://www.mdpi.com/2311-7524/9/9/1055Seed priming6-benzylaminopurine<i>Olea europeae</i> sproutsphytochemicalsantioxidant activity |
| spellingShingle | Samy Selim Ahlem Zrig Mha Albqmi Mohammad M. Al-Sanea Taghreed S. Alnusaire Mohammed S. Almuhayawi Soad K. Al Jaouni Shaimaa Hussein Mona Warrad Hamada AbdElgawad BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive Sprouts Horticulturae Seed priming 6-benzylaminopurine <i>Olea europeae</i> sprouts phytochemicals antioxidant activity |
| title | BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive Sprouts |
| title_full | BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive Sprouts |
| title_fullStr | BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive Sprouts |
| title_full_unstemmed | BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive Sprouts |
| title_short | BAP (6-Benzylaminopurine) Seed-Priming Enhanced Growth, Antioxidant Accumulation and Anthocyanin Metabolism in Olive Sprouts |
| title_sort | bap 6 benzylaminopurine seed priming enhanced growth antioxidant accumulation and anthocyanin metabolism in olive sprouts |
| topic | Seed priming 6-benzylaminopurine <i>Olea europeae</i> sprouts phytochemicals antioxidant activity |
| url | https://www.mdpi.com/2311-7524/9/9/1055 |
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