Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146.
The genomes of Pseudomonas aeruginosa isolates of the new sequence type ST-1146, three environmental (P37, P47 and P49) and one clinical (SD9) isolates, with differences in their antibiotic susceptibility profiles have been sequenced and analysed. The genomes were mapped against P. aeruginosa PAO1-U...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0107754 |
| _version_ | 1819100859863138304 |
|---|---|
| author | David Sánchez Margarita Gomila Antonio Bennasar Jorge Lalucat Elena García-Valdés |
| author_facet | David Sánchez Margarita Gomila Antonio Bennasar Jorge Lalucat Elena García-Valdés |
| author_sort | David Sánchez |
| collection | DOAJ |
| description | The genomes of Pseudomonas aeruginosa isolates of the new sequence type ST-1146, three environmental (P37, P47 and P49) and one clinical (SD9) isolates, with differences in their antibiotic susceptibility profiles have been sequenced and analysed. The genomes were mapped against P. aeruginosa PAO1-UW and UCBPP-PA14. The allelic profiles showed that the highest number of differences were in "Related to phage, transposon or plasmid" and "Secreted factors" categories. The clinical isolate showed a number of exclusive alleles greater than that for the environmental isolates. The phage Pf1 region in isolate SD9 accumulated the highest number of nucleotide substitutions. The ORF analysis of the four genomes assembled de novo indicated that the number of isolate-specific genes was higher in isolate SD9 (132 genes) than in isolates P37 (24 genes), P47 (16 genes) and P49 (21 genes). CRISPR elements were found in all isolates and SD9 showed differences in the spacer region. Genes related to bacteriophages F116 and H66 were found only in isolate SD9. Genome comparisons indicated that the isolates of ST-1146 are close related, and most genes implicated in pathogenicity are highly conserved, suggesting a genetic potential for infectivity in the environmental isolates similar to the clinical one. Phage-related genes are responsible of the main differences among the genomes of ST-1146 isolates. The role of bacteriophages has to be considered in the adaptation processes of isolates to the host and in microevolution studies. |
| first_indexed | 2024-12-22T01:09:28Z |
| format | Article |
| id | doaj.art-08704656d6b342369fb82258d78e1014 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T01:09:28Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-08704656d6b342369fb82258d78e10142022-12-21T18:44:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10775410.1371/journal.pone.0107754Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146.David SánchezMargarita GomilaAntonio BennasarJorge LalucatElena García-ValdésThe genomes of Pseudomonas aeruginosa isolates of the new sequence type ST-1146, three environmental (P37, P47 and P49) and one clinical (SD9) isolates, with differences in their antibiotic susceptibility profiles have been sequenced and analysed. The genomes were mapped against P. aeruginosa PAO1-UW and UCBPP-PA14. The allelic profiles showed that the highest number of differences were in "Related to phage, transposon or plasmid" and "Secreted factors" categories. The clinical isolate showed a number of exclusive alleles greater than that for the environmental isolates. The phage Pf1 region in isolate SD9 accumulated the highest number of nucleotide substitutions. The ORF analysis of the four genomes assembled de novo indicated that the number of isolate-specific genes was higher in isolate SD9 (132 genes) than in isolates P37 (24 genes), P47 (16 genes) and P49 (21 genes). CRISPR elements were found in all isolates and SD9 showed differences in the spacer region. Genes related to bacteriophages F116 and H66 were found only in isolate SD9. Genome comparisons indicated that the isolates of ST-1146 are close related, and most genes implicated in pathogenicity are highly conserved, suggesting a genetic potential for infectivity in the environmental isolates similar to the clinical one. Phage-related genes are responsible of the main differences among the genomes of ST-1146 isolates. The role of bacteriophages has to be considered in the adaptation processes of isolates to the host and in microevolution studies.https://doi.org/10.1371/journal.pone.0107754 |
| spellingShingle | David Sánchez Margarita Gomila Antonio Bennasar Jorge Lalucat Elena García-Valdés Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146. PLoS ONE |
| title | Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146. |
| title_full | Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146. |
| title_fullStr | Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146. |
| title_full_unstemmed | Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146. |
| title_short | Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146. |
| title_sort | genome analysis of environmental and clinical p aeruginosa isolates from sequence type 1146 |
| url | https://doi.org/10.1371/journal.pone.0107754 |
| work_keys_str_mv | AT davidsanchez genomeanalysisofenvironmentalandclinicalpaeruginosaisolatesfromsequencetype1146 AT margaritagomila genomeanalysisofenvironmentalandclinicalpaeruginosaisolatesfromsequencetype1146 AT antoniobennasar genomeanalysisofenvironmentalandclinicalpaeruginosaisolatesfromsequencetype1146 AT jorgelalucat genomeanalysisofenvironmentalandclinicalpaeruginosaisolatesfromsequencetype1146 AT elenagarciavaldes genomeanalysisofenvironmentalandclinicalpaeruginosaisolatesfromsequencetype1146 |