Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis
Several imaging techniques aim at identifying features of carotid plaque instability but come with limitations, such as the use of contrast agents, long examination times and poor portability. Multispectral optoacoustic tomography (MSOT) employs light and sound to resolve lipid and hemoglobin conten...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2021-09-01
|
Series: | Photoacoustics |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597921000446 |
_version_ | 1819149013672263680 |
---|---|
author | Angelos Karlas Michael Kallmayer Michael Bariotakis Nikolina-Alexia Fasoula Evangelos Liapis Fabien Hyafil Jaroslav Pelisek Moritz Wildgruber Hans-Henning Eckstein Vasilis Ntziachristos |
author_facet | Angelos Karlas Michael Kallmayer Michael Bariotakis Nikolina-Alexia Fasoula Evangelos Liapis Fabien Hyafil Jaroslav Pelisek Moritz Wildgruber Hans-Henning Eckstein Vasilis Ntziachristos |
author_sort | Angelos Karlas |
collection | DOAJ |
description | Several imaging techniques aim at identifying features of carotid plaque instability but come with limitations, such as the use of contrast agents, long examination times and poor portability. Multispectral optoacoustic tomography (MSOT) employs light and sound to resolve lipid and hemoglobin content, both features associated with plaque instability, in a label-free, fast and highly portable way. Herein, 5 patients with carotid atherosclerosis, 5 healthy volunteers and 2 excised plaques, were scanned with handheld MSOT. Spectral unmixing allowed visualization of lipid and hemoglobin content within three ROIs: whole arterial cross-section, plaque and arterial lumen. Calculation of the fat-blood-ratio (FBR) value within the ROIs enabled the differentiation between patients and healthy volunteers (P = 0.001) and between plaque and lumen in patients (P = 0.04). Our results introduce MSOT as a tool for molecular imaging of human carotid atherosclerosis and open new possibilities for research and clinical assessment of carotid plaques. |
first_indexed | 2024-12-22T13:54:52Z |
format | Article |
id | doaj.art-0871b348e645403ea7e0852f766358e6 |
institution | Directory Open Access Journal |
issn | 2213-5979 |
language | English |
last_indexed | 2024-12-22T13:54:52Z |
publishDate | 2021-09-01 |
publisher | Elsevier |
record_format | Article |
series | Photoacoustics |
spelling | doaj.art-0871b348e645403ea7e0852f766358e62022-12-21T18:23:34ZengElsevierPhotoacoustics2213-59792021-09-0123100283Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosisAngelos Karlas0Michael Kallmayer1Michael Bariotakis2Nikolina-Alexia Fasoula3Evangelos Liapis4Fabien Hyafil5Jaroslav Pelisek6Moritz Wildgruber7Hans-Henning Eckstein8Vasilis Ntziachristos9Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, Germany; Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Neuherberg, Germany; Clinic for Vascular and Endovascular Surgery, Technical University of Munich, Klinikum rechts der Isar, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, GermanyClinic for Vascular and Endovascular Surgery, Technical University of Munich, Klinikum rechts der Isar, Munich, GermanyChair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, Germany; Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Neuherberg, GermanyChair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, Germany; Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Neuherberg, GermanyChair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, Germany; Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Neuherberg, GermanyINSERM U1148, Laboratory for Vascular Translational Science (LVTS), DHU FIRE, University de Paris, Paris, France; Department of Nuclear Medicine, Bichat University Hospital, Assistance-Publique-Hôpitaux de Paris, Paris, FranceClinic for Vascular and Endovascular Surgery, Technical University of Munich, Klinikum rechts der Isar, Munich, Germany; Department of Vascular Surgery, University Hospital Zurich, Zurich, SwitzerlandDepartment of Radiology, University Hospital, LMU Munich, Munich, GermanyClinic for Vascular and Endovascular Surgery, Technical University of Munich, Klinikum rechts der Isar, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, GermanyChair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, Germany; Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Neuherberg, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany; Corresponding author at: Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, Germany.Several imaging techniques aim at identifying features of carotid plaque instability but come with limitations, such as the use of contrast agents, long examination times and poor portability. Multispectral optoacoustic tomography (MSOT) employs light and sound to resolve lipid and hemoglobin content, both features associated with plaque instability, in a label-free, fast and highly portable way. Herein, 5 patients with carotid atherosclerosis, 5 healthy volunteers and 2 excised plaques, were scanned with handheld MSOT. Spectral unmixing allowed visualization of lipid and hemoglobin content within three ROIs: whole arterial cross-section, plaque and arterial lumen. Calculation of the fat-blood-ratio (FBR) value within the ROIs enabled the differentiation between patients and healthy volunteers (P = 0.001) and between plaque and lumen in patients (P = 0.04). Our results introduce MSOT as a tool for molecular imaging of human carotid atherosclerosis and open new possibilities for research and clinical assessment of carotid plaques.http://www.sciencedirect.com/science/article/pii/S2213597921000446MSOTPhotoacoustic imagingCarotid plaqueStrokeCardiovascular diseaseMolecular imaging |
spellingShingle | Angelos Karlas Michael Kallmayer Michael Bariotakis Nikolina-Alexia Fasoula Evangelos Liapis Fabien Hyafil Jaroslav Pelisek Moritz Wildgruber Hans-Henning Eckstein Vasilis Ntziachristos Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis Photoacoustics MSOT Photoacoustic imaging Carotid plaque Stroke Cardiovascular disease Molecular imaging |
title | Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis |
title_full | Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis |
title_fullStr | Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis |
title_full_unstemmed | Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis |
title_short | Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis |
title_sort | multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis |
topic | MSOT Photoacoustic imaging Carotid plaque Stroke Cardiovascular disease Molecular imaging |
url | http://www.sciencedirect.com/science/article/pii/S2213597921000446 |
work_keys_str_mv | AT angeloskarlas multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT michaelkallmayer multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT michaelbariotakis multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT nikolinaalexiafasoula multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT evangelosliapis multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT fabienhyafil multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT jaroslavpelisek multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT moritzwildgruber multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT hanshenningeckstein multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis AT vasilisntziachristos multispectraloptoacoustictomographyoflipidandhemoglobincontrastinhumancarotidatherosclerosis |