Study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet [Setaria italica (L.) P. Beauv.]
<strong>Objective</strong> Identification of associated markers with important agricultural traits is one of the most important methods for accelerating the transfer of desirable traits to other genotypes and their tracking. The aim of this research was to study thegenetic diversity and...
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| Format: | Article |
| Language: | fas |
| Published: |
Shahid Bahonar University of Kerman
2020-02-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_2530_1f0efcc0b41c7e6d527d859f2eb34831.pdf |
| _version_ | 1828833322181066752 |
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| author | Babak Nakhoda Ghasem Mohammadi-Nejad Akram Aminizadeh |
| author_facet | Babak Nakhoda Ghasem Mohammadi-Nejad Akram Aminizadeh |
| author_sort | Babak Nakhoda |
| collection | DOAJ |
| description | <strong>Objective</strong>
Identification of associated markers with important agricultural traits is one of the most important methods for accelerating the transfer of desirable traits to other genotypes and their tracking. The aim of this research was to study thegenetic diversity and identification of molecular markers related to drought tolerance traits in foxtail millet [<em>Setaria italica</em> (L.) P. Beauv.]genotypes using the AFLP molecular marker.
<strong>Materials and methods</strong>
In this study, 21 genotypes of foxtail millet were studied in three growing seasons (2013, 2014 & 2016). Drought tolerance indices and correlation between them with grain yield and biological yield were calculated. In order to identify molecular markers associated with drought tolerance traits, association analysis was performed by using the Mixed Linear Model (MLM) and 12 primer combinations of AFLP. Also, marker indices and principal components analysis (PCA) were performed using GenAlEx 6.5 software
<strong>Results</strong>
The primer combinations used in this study generated a total of 443 scorable bands, of which 316 (71%) were polymorphic. The mean of polymorphism information content (PIC) was 0.24, the mean of Shannon index was 0.38, and the mean of Marker index was 6.22. Also, M-CTG/E-ACC, M-CAA/E-ACC and M-CTA/E-AAC were the most efficient combinations in investigating the diversity of genotypes studied. Based on the results of PCA, the first and second components justify 61.13 percent of the changes. The results of this study showed that HARM, GMP, and MP indices were the best indices for differentiation of drought tolerant samples, and M-CAA/E-AAC-14 and M-CTG/E-ACC-283 markers according to Grain yield and M-CTT/E-ACC-264 markers according to biological yield, were related to these indicators.
<strong>Conclusions</strong>
According to the results of this study, the HARM, GMP and MP indices could be considered as the best indicators for differentiation of drought tolerant foxtail millet samples. Also, molecular markers associated with these indices can be used to evaluate the drought tolerance of other genotypes in future breeding programs. |
| first_indexed | 2024-12-12T17:16:46Z |
| format | Article |
| id | doaj.art-08736245366743e4b5fc0b40890e8844 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-12T17:16:46Z |
| publishDate | 2020-02-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-08736245366743e4b5fc0b40890e88442022-12-22T00:17:46ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002020-02-0111419321810.22103/jab.2020.25302530Study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet [Setaria italica (L.) P. Beauv.]Babak Nakhoda0Ghasem Mohammadi-Nejad1Akram Aminizadeh2Babak Nakhoda (PhD) Head, Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Seed and Plant Improvement Campus, Shahid-FahmidehAssociate Professor of Plant Breeding, Faculty of Agriculture & Research and Technology Institute of Plant Production (RTIPP), Shahid-Bahonar University of Kerman, Kerman, Iran.University of Shahid Bahonar Kerman<strong>Objective</strong> Identification of associated markers with important agricultural traits is one of the most important methods for accelerating the transfer of desirable traits to other genotypes and their tracking. The aim of this research was to study thegenetic diversity and identification of molecular markers related to drought tolerance traits in foxtail millet [<em>Setaria italica</em> (L.) P. Beauv.]genotypes using the AFLP molecular marker. <strong>Materials and methods</strong> In this study, 21 genotypes of foxtail millet were studied in three growing seasons (2013, 2014 & 2016). Drought tolerance indices and correlation between them with grain yield and biological yield were calculated. In order to identify molecular markers associated with drought tolerance traits, association analysis was performed by using the Mixed Linear Model (MLM) and 12 primer combinations of AFLP. Also, marker indices and principal components analysis (PCA) were performed using GenAlEx 6.5 software <strong>Results</strong> The primer combinations used in this study generated a total of 443 scorable bands, of which 316 (71%) were polymorphic. The mean of polymorphism information content (PIC) was 0.24, the mean of Shannon index was 0.38, and the mean of Marker index was 6.22. Also, M-CTG/E-ACC, M-CAA/E-ACC and M-CTA/E-AAC were the most efficient combinations in investigating the diversity of genotypes studied. Based on the results of PCA, the first and second components justify 61.13 percent of the changes. The results of this study showed that HARM, GMP, and MP indices were the best indices for differentiation of drought tolerant samples, and M-CAA/E-AAC-14 and M-CTG/E-ACC-283 markers according to Grain yield and M-CTT/E-ACC-264 markers according to biological yield, were related to these indicators. <strong>Conclusions</strong> According to the results of this study, the HARM, GMP and MP indices could be considered as the best indicators for differentiation of drought tolerant foxtail millet samples. Also, molecular markers associated with these indices can be used to evaluate the drought tolerance of other genotypes in future breeding programs.https://jab.uk.ac.ir/article_2530_1f0efcc0b41c7e6d527d859f2eb34831.pdfaflp markerassociation analysisfoxtail millettolerance indicator |
| spellingShingle | Babak Nakhoda Ghasem Mohammadi-Nejad Akram Aminizadeh Study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet [Setaria italica (L.) P. Beauv.] مجله بیوتکنولوژی کشاورزی aflp marker association analysis foxtail millet tolerance indicator |
| title | Study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_full | Study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_fullStr | Study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_full_unstemmed | Study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_short | Study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_sort | study of genetic diversity and identification of informative molecular markers related to drought tolerance in foxtail millet setaria italica l p beauv |
| topic | aflp marker association analysis foxtail millet tolerance indicator |
| url | https://jab.uk.ac.ir/article_2530_1f0efcc0b41c7e6d527d859f2eb34831.pdf |
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