Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles
Magnetic nanoparticles offer multiple utilization possibilities in biomedicine. In this context, the interaction with cellular structures and their biological effects need to be understood and controlled for clinical safety. New magnetic nanoparticles containing metallic/carbidic iron and elemental...
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MDPI AG
2018-07-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/7/495 |
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| author | Mihaela Balas Florian Dumitrache Madalina Andreea Badea Claudiu Fleaca Anca Badoi Eugenia Tanasa Anca Dinischiotu |
| author_facet | Mihaela Balas Florian Dumitrache Madalina Andreea Badea Claudiu Fleaca Anca Badoi Eugenia Tanasa Anca Dinischiotu |
| author_sort | Mihaela Balas |
| collection | DOAJ |
| description | Magnetic nanoparticles offer multiple utilization possibilities in biomedicine. In this context, the interaction with cellular structures and their biological effects need to be understood and controlled for clinical safety. New magnetic nanoparticles containing metallic/carbidic iron and elemental silicon phases were synthesized by laser pyrolysis using Fe(CO)5 vapors and SiH4 gas as Fe and Si precursors, then passivated and coated with biocompatible agents, such as l-3,4-dihydroxyphenylalanine (l-DOPA) and sodium carboxymethyl cellulose (CMC-Na). The resulting magnetic nanoparticles were characterized by XRD, EDS, and TEM techniques. To evaluate their biocompatibility, doses ranging from 0–200 µg/mL hybrid Fe-Si nanoparticles were exposed to Caco2 cells for 24 and 72 h. Doses below 50 μg/mL of both l-DOPA and CMC-Na-coated Fe-Si nanoparticles induced no significant changes of cellular viability or membrane integrity. The cellular internalization of nanoparticles was dependent on their dispersion in culture medium and caused some changes of F-actin filaments organization after 72 h. However, reactive oxygen species were generated after exposure to 25 and 50 μg/mL of both Fe-Si nanoparticles types, inducing the increase of intracellular glutathione level and activation of transcription factor Nrf2. At nanoparticles doses below 50 μg/mL, Caco2 cells were able to counteract the oxidative stress by activating the cellular protection mechanisms. We concluded that in vitro biological responses to coated hybrid Fe-Si nanoparticles depended on particle synthesis conditions, surface coating, doses and incubation time. |
| first_indexed | 2024-04-12T10:52:53Z |
| format | Article |
| id | doaj.art-0874cf5f566a49cf88d7fdaab6bb43b8 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-04-12T10:52:53Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-0874cf5f566a49cf88d7fdaab6bb43b82022-12-22T03:36:09ZengMDPI AGNanomaterials2079-49912018-07-018749510.3390/nano8070495nano8070495Coating Dependent In Vitro Biocompatibility of New Fe-Si NanoparticlesMihaela Balas0Florian Dumitrache1Madalina Andreea Badea2Claudiu Fleaca3Anca Badoi4Eugenia Tanasa5Anca Dinischiotu6Department of Biochemistry and Molecular Biology, University of Bucharest, 91–95 Splaiul Independenţei, 050095 Bucharest, sector 5, RomaniaNational Institute for Lasers, Plasma and Radiation Physics (NILPRP), Atomistilor 409, 077125 Magurele, RomaniaDepartment of Biochemistry and Molecular Biology, University of Bucharest, 91–95 Splaiul Independenţei, 050095 Bucharest, sector 5, RomaniaNational Institute for Lasers, Plasma and Radiation Physics (NILPRP), Atomistilor 409, 077125 Magurele, RomaniaNational Institute for Lasers, Plasma and Radiation Physics (NILPRP), Atomistilor 409, 077125 Magurele, RomaniaDepartment of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Gh. Polizu 1-7, 11061 Bucharest, sector 1, RomaniaDepartment of Biochemistry and Molecular Biology, University of Bucharest, 91–95 Splaiul Independenţei, 050095 Bucharest, sector 5, RomaniaMagnetic nanoparticles offer multiple utilization possibilities in biomedicine. In this context, the interaction with cellular structures and their biological effects need to be understood and controlled for clinical safety. New magnetic nanoparticles containing metallic/carbidic iron and elemental silicon phases were synthesized by laser pyrolysis using Fe(CO)5 vapors and SiH4 gas as Fe and Si precursors, then passivated and coated with biocompatible agents, such as l-3,4-dihydroxyphenylalanine (l-DOPA) and sodium carboxymethyl cellulose (CMC-Na). The resulting magnetic nanoparticles were characterized by XRD, EDS, and TEM techniques. To evaluate their biocompatibility, doses ranging from 0–200 µg/mL hybrid Fe-Si nanoparticles were exposed to Caco2 cells for 24 and 72 h. Doses below 50 μg/mL of both l-DOPA and CMC-Na-coated Fe-Si nanoparticles induced no significant changes of cellular viability or membrane integrity. The cellular internalization of nanoparticles was dependent on their dispersion in culture medium and caused some changes of F-actin filaments organization after 72 h. However, reactive oxygen species were generated after exposure to 25 and 50 μg/mL of both Fe-Si nanoparticles types, inducing the increase of intracellular glutathione level and activation of transcription factor Nrf2. At nanoparticles doses below 50 μg/mL, Caco2 cells were able to counteract the oxidative stress by activating the cellular protection mechanisms. We concluded that in vitro biological responses to coated hybrid Fe-Si nanoparticles depended on particle synthesis conditions, surface coating, doses and incubation time.http://www.mdpi.com/2079-4991/8/7/495hybrid Fe-Si nanoparticleslaser pyrolysisCaco2 cellscytotoxicityoxidative stress |
| spellingShingle | Mihaela Balas Florian Dumitrache Madalina Andreea Badea Claudiu Fleaca Anca Badoi Eugenia Tanasa Anca Dinischiotu Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles Nanomaterials hybrid Fe-Si nanoparticles laser pyrolysis Caco2 cells cytotoxicity oxidative stress |
| title | Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles |
| title_full | Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles |
| title_fullStr | Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles |
| title_full_unstemmed | Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles |
| title_short | Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles |
| title_sort | coating dependent in vitro biocompatibility of new fe si nanoparticles |
| topic | hybrid Fe-Si nanoparticles laser pyrolysis Caco2 cells cytotoxicity oxidative stress |
| url | http://www.mdpi.com/2079-4991/8/7/495 |
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