| Summary: | Treatment of <i>Stenotrophomonas maltophilia</i> infections comprises of sulfamethoxazole/tripethoprim (SXT) or fluoroquinolones. We investigated antimicrobial resistance, presence of resistance genes (<i>sul1</i>, <i>smqnr</i>) and clonal dissemination in <i>S. maltophilia</i> from a university hospital. Among 62 isolates, 45 (73%) represented infection. Two isolates (3%) were resistant to SXT and three (5%) to levofloxacin. Twenty-nine isolates (47%), including two out of three levofloxacin-resistant, carried <i>smqnr</i>. Resistance of <i>S. maltophilia</i> was low and was not associated with <i>sul1</i> or <i>smqnr</i> carriage. Although high degree of genetic diversity was identified (29 pulsotypes), 22/62 (35.5%) strains were classified into four clones; clone b was associated with bacteraemias.
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