Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight Loss
The gut microbiota has been shown in recent years to be involved in the development and severity of type 2 diabetes (T2D). The aim of the present study was to test the effect of a 2-week functional food intervention on the gut microbiota composition in prediabetic individuals. A randomized double-bl...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/4/781 |
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| author | Ingrid S. Surono Abraham Simatupang Pratiwi D. Kusumo Priyo Waspodo Sanne Verbruggen Jessica Verhoeven Koen Venema |
| author_facet | Ingrid S. Surono Abraham Simatupang Pratiwi D. Kusumo Priyo Waspodo Sanne Verbruggen Jessica Verhoeven Koen Venema |
| author_sort | Ingrid S. Surono |
| collection | DOAJ |
| description | The gut microbiota has been shown in recent years to be involved in the development and severity of type 2 diabetes (T2D). The aim of the present study was to test the effect of a 2-week functional food intervention on the gut microbiota composition in prediabetic individuals. A randomized double-blind, cross-over trial was conducted on prediabetic subjects. Fifteen volunteers were provided products made of: (i) 50% taro flour + 50% wheat flour; (ii) these products and the probiotic <i>L. plantarum</i> IS-10506; or (iii) these products with beetroot adsorbed for a period of 2 weeks with 2 weeks wash-out in between. Stool and blood samples were taken at each baseline and after each of the interventions. The gut microbiota composition was evaluated by sequencing the V3–V4 region of the 16S rRNA gene and anthropometric measures were recorded. The total weight loss over the entire period ranged from 0.5 to 11 kg. The next-generation sequencing showed a highly personalized microbiota composition. In the principal coordinate analyses, the samples of each individual clustered closer together than the samples of each treatment. For six individuals, the samples clustered closely together, indicating a stable microbiota. For nine individuals, the microbiota was less resilient and, depending on the intervention, the beta-diversity transiently differed greatly only to return to the composition close to the baseline during the wash-out. The statistical analyses showed that 202 of the total 304 taxa were significantly different between the participants. Only <i>Butyricimonas</i> could be correlated with taro ingestion. The results of the study show that the highly variable interindividual variation observed in the gut microbiota of the participants clouded any gut microbiota modulation that might be present due to the functional food interventions. |
| first_indexed | 2024-03-09T21:17:30Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T21:17:30Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-087736f7d8b44d9eb9659c246cbd36022023-11-23T21:28:17ZengMDPI AGNutrients2072-66432022-02-0114478110.3390/nu14040781Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight LossIngrid S. Surono0Abraham Simatupang1Pratiwi D. Kusumo2Priyo Waspodo3Sanne Verbruggen4Jessica Verhoeven5Koen Venema6Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta 11480, IndonesiaFaculty of Medicine, Universitas Kristen Indonesia, Jakarta 13630, IndonesiaFaculty of Medicine, Universitas Kristen Indonesia, Jakarta 13630, IndonesiaFood Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta 11480, IndonesiaCentre for Healthy Eating & Food Innovation, Maastricht University—Campus Venlo, 5928 SZ Venlo, The NetherlandsCentre for Healthy Eating & Food Innovation, Maastricht University—Campus Venlo, 5928 SZ Venlo, The NetherlandsCentre for Healthy Eating & Food Innovation, Maastricht University—Campus Venlo, 5928 SZ Venlo, The NetherlandsThe gut microbiota has been shown in recent years to be involved in the development and severity of type 2 diabetes (T2D). The aim of the present study was to test the effect of a 2-week functional food intervention on the gut microbiota composition in prediabetic individuals. A randomized double-blind, cross-over trial was conducted on prediabetic subjects. Fifteen volunteers were provided products made of: (i) 50% taro flour + 50% wheat flour; (ii) these products and the probiotic <i>L. plantarum</i> IS-10506; or (iii) these products with beetroot adsorbed for a period of 2 weeks with 2 weeks wash-out in between. Stool and blood samples were taken at each baseline and after each of the interventions. The gut microbiota composition was evaluated by sequencing the V3–V4 region of the 16S rRNA gene and anthropometric measures were recorded. The total weight loss over the entire period ranged from 0.5 to 11 kg. The next-generation sequencing showed a highly personalized microbiota composition. In the principal coordinate analyses, the samples of each individual clustered closer together than the samples of each treatment. For six individuals, the samples clustered closely together, indicating a stable microbiota. For nine individuals, the microbiota was less resilient and, depending on the intervention, the beta-diversity transiently differed greatly only to return to the composition close to the baseline during the wash-out. The statistical analyses showed that 202 of the total 304 taxa were significantly different between the participants. Only <i>Butyricimonas</i> could be correlated with taro ingestion. The results of the study show that the highly variable interindividual variation observed in the gut microbiota of the participants clouded any gut microbiota modulation that might be present due to the functional food interventions.https://www.mdpi.com/2072-6643/14/4/781taroprobiotic<i>L. plantarum</i> IS-10506beetrootgut microbiotaprediabetes |
| spellingShingle | Ingrid S. Surono Abraham Simatupang Pratiwi D. Kusumo Priyo Waspodo Sanne Verbruggen Jessica Verhoeven Koen Venema Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight Loss Nutrients taro probiotic <i>L. plantarum</i> IS-10506 beetroot gut microbiota prediabetes |
| title | Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight Loss |
| title_full | Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight Loss |
| title_fullStr | Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight Loss |
| title_full_unstemmed | Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight Loss |
| title_short | Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight Loss |
| title_sort | effect of different functional food supplements on the gut microbiota of prediabetic indonesian individuals during weight loss |
| topic | taro probiotic <i>L. plantarum</i> IS-10506 beetroot gut microbiota prediabetes |
| url | https://www.mdpi.com/2072-6643/14/4/781 |
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