Management of foot rot of citrus (Citrus jambhiri spp.) using biocontrol agents
Citrus holds an important place in promoting the horticultural wealth and economy of India. Foot rot caused by Phytophthora spp. is a widespread problem of the citrus nursery. Citrus jambhiri, a widely used rootstock in nursery production, is found susceptible to the Phytophthora spp. Hence, the pr...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2022-09-01
|
| Series: | The Indian Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/122917 |
| _version_ | 1811169140750155776 |
|---|---|
| author | AJAY KUMAR CHOUDHARY NARINDER SINGH DALJEET SINGH BUTTAR |
| author_facet | AJAY KUMAR CHOUDHARY NARINDER SINGH DALJEET SINGH BUTTAR |
| author_sort | AJAY KUMAR CHOUDHARY |
| collection | DOAJ |
| description |
Citrus holds an important place in promoting the horticultural wealth and economy of India. Foot rot caused by Phytophthora spp. is a widespread problem of the citrus nursery. Citrus jambhiri, a widely used rootstock in nursery production, is found susceptible to the Phytophthora spp. Hence, the present study was carried out to evaluate antagonistic activity of Trichoderma spp. isolates against Phytophthora nicotianae var. parasitica causing foot rot in C. jambhiri under lab and net-house conditions. Seven isolates (parent and mutant) of Trichoderma spp. were tested in vitro. Amongst them, T20 mutant (Trichoderma asperellum) exhibited maximum mycoparasitism, volatile activity and non-volatile activity, i.e. 83.70%, 79.26% and 84.81%, respectively. The same T20 mutant (T. Asperellum) also showed maximum glucanase activity, i.e. 1.98 unit/ml. Further, talc-based bio formulations of T. asperellum T20 isolate (parent and mutant) were tested under net-house conditions over a period of two years (2016–2017 and 2017–2018) at the research farm of Punjab Agricultural University, Ludhiana, Punjab. Amongst all the treatments, the application of T20 mutant (T. asperellum) (seed + soil 15 g) had minimum disease incidence (13.33%), maximum disease control (83.30%) and maximum growth promotion i.e. shoot length (37.67 cm) and root length (30.67 cm). Based on our findings, T. asperellum T20 mutant strain used as seed + soil treatment was able to effectively manage the foot rot, in C. jambhiri nursery under net-house conditions and also promoted the plant growth.
|
| first_indexed | 2024-04-10T16:37:25Z |
| format | Article |
| id | doaj.art-087c0ddb234f4025a08d6b99ebeb00e6 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T16:37:25Z |
| publishDate | 2022-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-087c0ddb234f4025a08d6b99ebeb00e62023-02-08T11:09:05ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192022-09-0192910.56093/ijas.v92i9.122917Management of foot rot of citrus (Citrus jambhiri spp.) using biocontrol agentsAJAY KUMAR CHOUDHARY0NARINDER SINGH1DALJEET SINGH BUTTAR2Punjab Agricultural University, Ludhiana, Punjab 141 004, IndiaPunjab Agricultural University, Ludhiana, Punjab 141 004, IndiaPunjab Agricultural University, Ludhiana, Punjab 141 004, India Citrus holds an important place in promoting the horticultural wealth and economy of India. Foot rot caused by Phytophthora spp. is a widespread problem of the citrus nursery. Citrus jambhiri, a widely used rootstock in nursery production, is found susceptible to the Phytophthora spp. Hence, the present study was carried out to evaluate antagonistic activity of Trichoderma spp. isolates against Phytophthora nicotianae var. parasitica causing foot rot in C. jambhiri under lab and net-house conditions. Seven isolates (parent and mutant) of Trichoderma spp. were tested in vitro. Amongst them, T20 mutant (Trichoderma asperellum) exhibited maximum mycoparasitism, volatile activity and non-volatile activity, i.e. 83.70%, 79.26% and 84.81%, respectively. The same T20 mutant (T. Asperellum) also showed maximum glucanase activity, i.e. 1.98 unit/ml. Further, talc-based bio formulations of T. asperellum T20 isolate (parent and mutant) were tested under net-house conditions over a period of two years (2016–2017 and 2017–2018) at the research farm of Punjab Agricultural University, Ludhiana, Punjab. Amongst all the treatments, the application of T20 mutant (T. asperellum) (seed + soil 15 g) had minimum disease incidence (13.33%), maximum disease control (83.30%) and maximum growth promotion i.e. shoot length (37.67 cm) and root length (30.67 cm). Based on our findings, T. asperellum T20 mutant strain used as seed + soil treatment was able to effectively manage the foot rot, in C. jambhiri nursery under net-house conditions and also promoted the plant growth. https://epubs.icar.org.in/index.php/IJAgS/article/view/122917Citrus jambhiri, Foot rot, Trichoderma asperellum T20 mutant |
| spellingShingle | AJAY KUMAR CHOUDHARY NARINDER SINGH DALJEET SINGH BUTTAR Management of foot rot of citrus (Citrus jambhiri spp.) using biocontrol agents The Indian Journal of Agricultural Sciences Citrus jambhiri, Foot rot, Trichoderma asperellum T20 mutant |
| title | Management of foot rot of citrus (Citrus jambhiri spp.) using biocontrol agents |
| title_full | Management of foot rot of citrus (Citrus jambhiri spp.) using biocontrol agents |
| title_fullStr | Management of foot rot of citrus (Citrus jambhiri spp.) using biocontrol agents |
| title_full_unstemmed | Management of foot rot of citrus (Citrus jambhiri spp.) using biocontrol agents |
| title_short | Management of foot rot of citrus (Citrus jambhiri spp.) using biocontrol agents |
| title_sort | management of foot rot of citrus citrus jambhiri spp using biocontrol agents |
| topic | Citrus jambhiri, Foot rot, Trichoderma asperellum T20 mutant |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/122917 |
| work_keys_str_mv | AT ajaykumarchoudhary managementoffootrotofcitruscitrusjambhirisppusingbiocontrolagents AT narindersingh managementoffootrotofcitruscitrusjambhirisppusingbiocontrolagents AT daljeetsinghbuttar managementoffootrotofcitruscitrusjambhirisppusingbiocontrolagents |