Different Alterations in Gut Microbiota between <i>Bifidobacterium longum</i> and Fecal Microbiota Transplantation Treatments in Propionic Acid Rat Model of Autism
Autism spectrum disorders (ASD) consist of a range of neurodevelopmental conditions accompanied by dysbiosis of gut microbiota. Therefore, a number of microbiota manipulation strategies were developed to restore their balance. However, a comprehensive comparison of the various methods on gut microbi...
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MDPI AG
2022-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/3/608 |
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| author | Turki S. Abujamel Norah M. Al-Otaibi Sameera Abuaish Rahaf H. AlHarbi Mushref B. Assas Saleha Ahmad Alzahrani Sohailah Masoud Alotaibi Afaf El-Ansary Kawther Aabed |
| author_facet | Turki S. Abujamel Norah M. Al-Otaibi Sameera Abuaish Rahaf H. AlHarbi Mushref B. Assas Saleha Ahmad Alzahrani Sohailah Masoud Alotaibi Afaf El-Ansary Kawther Aabed |
| author_sort | Turki S. Abujamel |
| collection | DOAJ |
| description | Autism spectrum disorders (ASD) consist of a range of neurodevelopmental conditions accompanied by dysbiosis of gut microbiota. Therefore, a number of microbiota manipulation strategies were developed to restore their balance. However, a comprehensive comparison of the various methods on gut microbiota is still lacking. Here, we evaluated the effect of <i>Bifidobacterium</i> (BF) treatment and fecal microbiota transplantation (FT) on gut microbiota in a propionic acid (PPA) rat model of autism using 16S rRNA sequencing. Following PPA treatment, gut microbiota showed depletion of Bacteroidia and <i>Akkermansia</i> accompanied by a concomitant increase of <i>Streptococcus</i>, <i>Lachnospiraceae</i>, and <i>Paraeggerthella</i>. The dysbiosis was predicted to cause increased levels of porphyrin metabolism and impairments of acyl-CoA thioesterase and ubiquinone biosynthesis. On the contrary, BF and FT treatments resulted in a distinct increase of <i>Clostridium</i>, <i>Bifidobacterium</i>, <i>Marvinbryantia</i>, <i>Butyricicoccus</i>, and <i>Dorea</i>. The taxa in BF group positively correlated with vitamin B12 and flagella biosynthesis, while FT mainly enriched flagella biosynthesis. In contrast, BF and FT treatments negatively correlated with succinate biosynthesis, pyruvate metabolism, nitrogen metabolism, beta-Lactam resistance, and peptidoglycan biosynthesis. Therefore, the present study demonstrated that BF and FT treatments restored the PPA-induced dysbiosis in a treatment-specific manner. |
| first_indexed | 2024-03-09T23:21:06Z |
| format | Article |
| id | doaj.art-087f4db63f56410fb477eef1d2a0fa2a |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T23:21:06Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-087f4db63f56410fb477eef1d2a0fa2a2023-11-23T17:26:40ZengMDPI AGNutrients2072-66432022-01-0114360810.3390/nu14030608Different Alterations in Gut Microbiota between <i>Bifidobacterium longum</i> and Fecal Microbiota Transplantation Treatments in Propionic Acid Rat Model of AutismTurki S. Abujamel0Norah M. Al-Otaibi1Sameera Abuaish2Rahaf H. AlHarbi3Mushref B. Assas4Saleha Ahmad Alzahrani5Sohailah Masoud Alotaibi6Afaf El-Ansary7Kawther Aabed8Vaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Basic Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaVaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaCentral Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, P.O. Box 22452, Riyadh 11472, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaAutism spectrum disorders (ASD) consist of a range of neurodevelopmental conditions accompanied by dysbiosis of gut microbiota. Therefore, a number of microbiota manipulation strategies were developed to restore their balance. However, a comprehensive comparison of the various methods on gut microbiota is still lacking. Here, we evaluated the effect of <i>Bifidobacterium</i> (BF) treatment and fecal microbiota transplantation (FT) on gut microbiota in a propionic acid (PPA) rat model of autism using 16S rRNA sequencing. Following PPA treatment, gut microbiota showed depletion of Bacteroidia and <i>Akkermansia</i> accompanied by a concomitant increase of <i>Streptococcus</i>, <i>Lachnospiraceae</i>, and <i>Paraeggerthella</i>. The dysbiosis was predicted to cause increased levels of porphyrin metabolism and impairments of acyl-CoA thioesterase and ubiquinone biosynthesis. On the contrary, BF and FT treatments resulted in a distinct increase of <i>Clostridium</i>, <i>Bifidobacterium</i>, <i>Marvinbryantia</i>, <i>Butyricicoccus</i>, and <i>Dorea</i>. The taxa in BF group positively correlated with vitamin B12 and flagella biosynthesis, while FT mainly enriched flagella biosynthesis. In contrast, BF and FT treatments negatively correlated with succinate biosynthesis, pyruvate metabolism, nitrogen metabolism, beta-Lactam resistance, and peptidoglycan biosynthesis. Therefore, the present study demonstrated that BF and FT treatments restored the PPA-induced dysbiosis in a treatment-specific manner.https://www.mdpi.com/2072-6643/14/3/608autistic spectrum disordersprobiotics<i>Bifidobacterium longum</i>microbiotafecal microbiota transplantation |
| spellingShingle | Turki S. Abujamel Norah M. Al-Otaibi Sameera Abuaish Rahaf H. AlHarbi Mushref B. Assas Saleha Ahmad Alzahrani Sohailah Masoud Alotaibi Afaf El-Ansary Kawther Aabed Different Alterations in Gut Microbiota between <i>Bifidobacterium longum</i> and Fecal Microbiota Transplantation Treatments in Propionic Acid Rat Model of Autism Nutrients autistic spectrum disorders probiotics <i>Bifidobacterium longum</i> microbiota fecal microbiota transplantation |
| title | Different Alterations in Gut Microbiota between <i>Bifidobacterium longum</i> and Fecal Microbiota Transplantation Treatments in Propionic Acid Rat Model of Autism |
| title_full | Different Alterations in Gut Microbiota between <i>Bifidobacterium longum</i> and Fecal Microbiota Transplantation Treatments in Propionic Acid Rat Model of Autism |
| title_fullStr | Different Alterations in Gut Microbiota between <i>Bifidobacterium longum</i> and Fecal Microbiota Transplantation Treatments in Propionic Acid Rat Model of Autism |
| title_full_unstemmed | Different Alterations in Gut Microbiota between <i>Bifidobacterium longum</i> and Fecal Microbiota Transplantation Treatments in Propionic Acid Rat Model of Autism |
| title_short | Different Alterations in Gut Microbiota between <i>Bifidobacterium longum</i> and Fecal Microbiota Transplantation Treatments in Propionic Acid Rat Model of Autism |
| title_sort | different alterations in gut microbiota between i bifidobacterium longum i and fecal microbiota transplantation treatments in propionic acid rat model of autism |
| topic | autistic spectrum disorders probiotics <i>Bifidobacterium longum</i> microbiota fecal microbiota transplantation |
| url | https://www.mdpi.com/2072-6643/14/3/608 |
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