Antibacterial effect of cell-free supernatant fraction from Lactobacillus paracasei CH88 against Gardnerella vaginalis
Abstract Bacterial vaginosis (BV) is the most common vaginal infection in reproductive women, which is characterized by depleted level of lactic acid bacteria and overgrowth of anaerobes such as Gardnerella vaginalis spp. Lactic acid bacteria have been known to be beneficial for amelioration of BV,...
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| Format: | Article |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-08808-7 |
| _version_ | 1818774504506130432 |
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| author | Eun Chae Moon Myeong Soo Park Taehwan Lim Ryun Hee Kim Geun Eog Ji Sun Young Kim Keum Taek Hwang |
| author_facet | Eun Chae Moon Myeong Soo Park Taehwan Lim Ryun Hee Kim Geun Eog Ji Sun Young Kim Keum Taek Hwang |
| author_sort | Eun Chae Moon |
| collection | DOAJ |
| description | Abstract Bacterial vaginosis (BV) is the most common vaginal infection in reproductive women, which is characterized by depleted level of lactic acid bacteria and overgrowth of anaerobes such as Gardnerella vaginalis spp. Lactic acid bacteria have been known to be beneficial for amelioration of BV, since they produce antimicrobial substances against G. vaginalis spp. The objectives of this study were to characterize different fractions of cell-free supernatant of Lactobacillus paracasei CH88 (LCFS) and investigate antibacterial activity of the LCFS fractions against G. vaginalis in-vitro and in-vivo. Antibacterial activity of the LCFS was stable during thermal treatment up to 120 °C for 30 min and maintained at pH ranging from 3.0 to 13.0 except pH 5.0. Fraction below 3 kDa of the LCFS partially lost its antibacterial activity after treatment with proteolytic enzymes. Precipitated protein fraction below 3 kDa of the LCFS (< 3 kDa LCFSP) inhibited the growth and biofilm formation of G. vaginalis. Treatment of L. paracasei CH88 or the < 3 kDa LCFSP attenuated G. vaginalis-induced BV in mice by inhibiting the growth of G. vaginalis, reducing exfoliation of vaginal epithelial cells, and regulating immune response. These results suggest that L. paracasei CH88 may have potential in ameliorating G. vaginalis-induced BV. |
| first_indexed | 2024-12-18T10:42:12Z |
| format | Article |
| id | doaj.art-08801f1e0bde458396216d545bbe62d8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T10:42:12Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-08801f1e0bde458396216d545bbe62d82022-12-21T21:10:37ZengNature PortfolioScientific Reports2045-23222022-03-0112111010.1038/s41598-022-08808-7Antibacterial effect of cell-free supernatant fraction from Lactobacillus paracasei CH88 against Gardnerella vaginalisEun Chae Moon0Myeong Soo Park1Taehwan Lim2Ryun Hee Kim3Geun Eog Ji4Sun Young Kim5Keum Taek Hwang6Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National UniversityResearch Center, BIFIDO Co., LtdDepartment of Biomedical Engineering, Tufts UniversityDepartment of Food and Nutrition, and Research Institute of Human Ecology, Seoul National UniversityResearch Center, BIFIDO Co., LtdResearch Center, BIFIDO Co., LtdDepartment of Food and Nutrition, and Research Institute of Human Ecology, Seoul National UniversityAbstract Bacterial vaginosis (BV) is the most common vaginal infection in reproductive women, which is characterized by depleted level of lactic acid bacteria and overgrowth of anaerobes such as Gardnerella vaginalis spp. Lactic acid bacteria have been known to be beneficial for amelioration of BV, since they produce antimicrobial substances against G. vaginalis spp. The objectives of this study were to characterize different fractions of cell-free supernatant of Lactobacillus paracasei CH88 (LCFS) and investigate antibacterial activity of the LCFS fractions against G. vaginalis in-vitro and in-vivo. Antibacterial activity of the LCFS was stable during thermal treatment up to 120 °C for 30 min and maintained at pH ranging from 3.0 to 13.0 except pH 5.0. Fraction below 3 kDa of the LCFS partially lost its antibacterial activity after treatment with proteolytic enzymes. Precipitated protein fraction below 3 kDa of the LCFS (< 3 kDa LCFSP) inhibited the growth and biofilm formation of G. vaginalis. Treatment of L. paracasei CH88 or the < 3 kDa LCFSP attenuated G. vaginalis-induced BV in mice by inhibiting the growth of G. vaginalis, reducing exfoliation of vaginal epithelial cells, and regulating immune response. These results suggest that L. paracasei CH88 may have potential in ameliorating G. vaginalis-induced BV.https://doi.org/10.1038/s41598-022-08808-7 |
| spellingShingle | Eun Chae Moon Myeong Soo Park Taehwan Lim Ryun Hee Kim Geun Eog Ji Sun Young Kim Keum Taek Hwang Antibacterial effect of cell-free supernatant fraction from Lactobacillus paracasei CH88 against Gardnerella vaginalis Scientific Reports |
| title | Antibacterial effect of cell-free supernatant fraction from Lactobacillus paracasei CH88 against Gardnerella vaginalis |
| title_full | Antibacterial effect of cell-free supernatant fraction from Lactobacillus paracasei CH88 against Gardnerella vaginalis |
| title_fullStr | Antibacterial effect of cell-free supernatant fraction from Lactobacillus paracasei CH88 against Gardnerella vaginalis |
| title_full_unstemmed | Antibacterial effect of cell-free supernatant fraction from Lactobacillus paracasei CH88 against Gardnerella vaginalis |
| title_short | Antibacterial effect of cell-free supernatant fraction from Lactobacillus paracasei CH88 against Gardnerella vaginalis |
| title_sort | antibacterial effect of cell free supernatant fraction from lactobacillus paracasei ch88 against gardnerella vaginalis |
| url | https://doi.org/10.1038/s41598-022-08808-7 |
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