Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022
Abstract Toxoplasmosis is a frequent infection among the human population. The infection can cause devastating complications for the fetus during pregnancy. The present study aimed to determine the serological and molecular prevalence of the infection and molecular characterization of Toxoplasma gon...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-024-09265-5 |
| _version_ | 1797199586310225920 |
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| author | Nasim Bashour Arash Aminpour Shabnam Vazifehkhah Rasool Jafari |
| author_facet | Nasim Bashour Arash Aminpour Shabnam Vazifehkhah Rasool Jafari |
| author_sort | Nasim Bashour |
| collection | DOAJ |
| description | Abstract Toxoplasmosis is a frequent infection among the human population. The infection can cause devastating complications for the fetus during pregnancy. The present study aimed to determine the serological and molecular prevalence of the infection and molecular characterization of Toxoplasma gondii isolates among pregnant women referred to Kowsar Hospital, Urmia, Iran. In a cross-sectional study, 340 blood samples were collected from pregnant women referred to Kowsar Hospital, Urmia, Iran from May to July 2022. Anti-T. gondii IgG and IgM seropositivity were determined by enzyme-linked immunosorbent assay. PCR was carried out by targeting the GRA6 gene of the parasite on all patients’ buffy coats. Anti-T. gondii IgG and IgM antibodies were positive in two (0.6%) women, and 101 (29.7%) women had anti-T. gondii IgG and 70.3% were seronegative. PCR was positive in two IgM-positive women, and both isolates belonged to T. gondii carrying the GRA6 allele of lineage I. The risk of infection was significantly higher in women who had constant contact with cats and soil, and who were residents of rural areas. The two IgM-positive women were asymptomatic regarding acute toxoplasmosis. According to the results of the present study, the prevalence of toxoplasmosis in pregnant women in Urmia is similar to its prevalence in other areas in northwestern Iran, and despite the low prevalence of acute infection, it should not be ignored. |
| first_indexed | 2024-04-24T07:18:06Z |
| format | Article |
| id | doaj.art-0885c3c8438a452f94b83915e3c3e15f |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-24T07:18:06Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-0885c3c8438a452f94b83915e3c3e15f2024-04-21T11:10:22ZengBMCBMC Infectious Diseases1471-23342024-04-012411910.1186/s12879-024-09265-5Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022Nasim Bashour0Arash Aminpour1Shabnam Vazifehkhah2Rasool Jafari3Department of Parasitology and Mycology, School of Medicine, Urmia University of Medical SciencesDepartment of Parasitology and Mycology, School of Medicine, Urmia University of Medical SciencesDepartment of Obstetrics and Gynecology, School of Medicine, Urmia University of Medical SciencesDepartment of Parasitology and Mycology, School of Medicine, Urmia University of Medical SciencesAbstract Toxoplasmosis is a frequent infection among the human population. The infection can cause devastating complications for the fetus during pregnancy. The present study aimed to determine the serological and molecular prevalence of the infection and molecular characterization of Toxoplasma gondii isolates among pregnant women referred to Kowsar Hospital, Urmia, Iran. In a cross-sectional study, 340 blood samples were collected from pregnant women referred to Kowsar Hospital, Urmia, Iran from May to July 2022. Anti-T. gondii IgG and IgM seropositivity were determined by enzyme-linked immunosorbent assay. PCR was carried out by targeting the GRA6 gene of the parasite on all patients’ buffy coats. Anti-T. gondii IgG and IgM antibodies were positive in two (0.6%) women, and 101 (29.7%) women had anti-T. gondii IgG and 70.3% were seronegative. PCR was positive in two IgM-positive women, and both isolates belonged to T. gondii carrying the GRA6 allele of lineage I. The risk of infection was significantly higher in women who had constant contact with cats and soil, and who were residents of rural areas. The two IgM-positive women were asymptomatic regarding acute toxoplasmosis. According to the results of the present study, the prevalence of toxoplasmosis in pregnant women in Urmia is similar to its prevalence in other areas in northwestern Iran, and despite the low prevalence of acute infection, it should not be ignored.https://doi.org/10.1186/s12879-024-09265-5Toxoplasma gondiiAcute infectionGenotypePregnant womenIran |
| spellingShingle | Nasim Bashour Arash Aminpour Shabnam Vazifehkhah Rasool Jafari Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022 BMC Infectious Diseases Toxoplasma gondii Acute infection Genotype Pregnant women Iran |
| title | Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022 |
| title_full | Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022 |
| title_fullStr | Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022 |
| title_full_unstemmed | Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022 |
| title_short | Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022 |
| title_sort | seromolecular study on the prevalence and risk factors of toxoplasma gondii infection in pregnant women referred to a gynecology hospital in urmia northwest part of iran in 2022 |
| topic | Toxoplasma gondii Acute infection Genotype Pregnant women Iran |
| url | https://doi.org/10.1186/s12879-024-09265-5 |
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