Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats
At present, gold nanoparticles (GNPs) are widely used in biomedical applications such as cancer diagnostics and therapy. Accordingly, the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention. Here, we report the effects of prolonged peroral ad...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2016-01-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | http://www.worldscientific.com/doi/pdf/10.1142/S1793545816400046 |
| _version_ | 1811241999154544640 |
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| author | Alla B. Bucharskaya Svetlana S. Pakhomy Olga V. Zlobina Galina N. Maslyakova Olga V. Matveeva Irina O. Bugaeva Nikita A. Navolokin Boris N. Khlebtsov Vladimir A. Bogatyrev Nikolai G. Khlebtsov Valery V. Tuchin |
| author_facet | Alla B. Bucharskaya Svetlana S. Pakhomy Olga V. Zlobina Galina N. Maslyakova Olga V. Matveeva Irina O. Bugaeva Nikita A. Navolokin Boris N. Khlebtsov Vladimir A. Bogatyrev Nikolai G. Khlebtsov Valery V. Tuchin |
| author_sort | Alla B. Bucharskaya |
| collection | DOAJ |
| description | At present, gold nanoparticles (GNPs) are widely used in biomedical applications such as cancer diagnostics and therapy. Accordingly, the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention. Here, we report the effects of prolonged peroral administration of GNPs with different sizes (2, 15 and 50nm) on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals. The experiment was conducted on 24 white mongrel male rats weighing 180–220g, gold nanospheres sizes 2, 15 and 50nm were administered orally for 15 days at a dosage of 190μg/kg of animal body weight. The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability. The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood, especially pronounced after administration of GNPs with size of 50nm. The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow. The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes, which were showed as the increasing number of immunoblasts and large lymphocytes. The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration, proliferation and differentiation of immune cells indicate the presence of immunostimulation effect of GNPs. |
| first_indexed | 2024-04-12T13:44:29Z |
| format | Article |
| id | doaj.art-0889308b41724bafac9add7059e69cb6 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-04-12T13:44:29Z |
| publishDate | 2016-01-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-0889308b41724bafac9add7059e69cb62022-12-22T03:30:43ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052016-01-01911640004-11640004-1010.1142/S179354581640004610.1142/S1793545816400046Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in ratsAlla B. Bucharskaya0Svetlana S. Pakhomy1Olga V. Zlobina2Galina N. Maslyakova3Olga V. Matveeva4Irina O. Bugaeva5Nikita A. Navolokin6Boris N. Khlebtsov7Vladimir A. Bogatyrev8Nikolai G. Khlebtsov9Valery V. Tuchin10Saratov State Medical University, 112, B. Kazach’ya str., Saratov 410012, RussiaSaratov State Medical University, 112, B. Kazach’ya str., Saratov 410012, RussiaSaratov State Medical University, 112, B. Kazach’ya str., Saratov 410012, RussiaSaratov State Medical University, 112, B. Kazach’ya str., Saratov 410012, RussiaSaratov State Medical University, 112, B. Kazach’ya str., Saratov 410012, RussiaSaratov State Medical University, 112, B. Kazach’ya str., Saratov 410012, RussiaSaratov State Medical University, 112, B. Kazach’ya str., Saratov 410012, RussiaInstitute of Biochemistry and Physiology of Plants and Microorganisms RAS, 13, Entuziastov Prospekt., Saratov 410049, RussiaInstitute of Biochemistry and Physiology of Plants and Microorganisms RAS, 13, Entuziastov Prospekt., Saratov 410049, RussiaInstitute of Biochemistry and Physiology of Plants and Microorganisms RAS, 13, Entuziastov Prospekt., Saratov 410049, RussiaSaratov State University 83, Astrakhanskaya str., Saratov 410012, RussiaAt present, gold nanoparticles (GNPs) are widely used in biomedical applications such as cancer diagnostics and therapy. Accordingly, the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention. Here, we report the effects of prolonged peroral administration of GNPs with different sizes (2, 15 and 50nm) on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals. The experiment was conducted on 24 white mongrel male rats weighing 180–220g, gold nanospheres sizes 2, 15 and 50nm were administered orally for 15 days at a dosage of 190μg/kg of animal body weight. The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability. The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood, especially pronounced after administration of GNPs with size of 50nm. The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow. The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes, which were showed as the increasing number of immunoblasts and large lymphocytes. The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration, proliferation and differentiation of immune cells indicate the presence of immunostimulation effect of GNPs.http://www.worldscientific.com/doi/pdf/10.1142/S1793545816400046Gold nanoparticleslymphoid organsmorphological changesbone marrow examination |
| spellingShingle | Alla B. Bucharskaya Svetlana S. Pakhomy Olga V. Zlobina Galina N. Maslyakova Olga V. Matveeva Irina O. Bugaeva Nikita A. Navolokin Boris N. Khlebtsov Vladimir A. Bogatyrev Nikolai G. Khlebtsov Valery V. Tuchin Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats Journal of Innovative Optical Health Sciences Gold nanoparticles lymphoid organs morphological changes bone marrow examination |
| title | Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats |
| title_full | Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats |
| title_fullStr | Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats |
| title_full_unstemmed | Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats |
| title_short | Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats |
| title_sort | alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats |
| topic | Gold nanoparticles lymphoid organs morphological changes bone marrow examination |
| url | http://www.worldscientific.com/doi/pdf/10.1142/S1793545816400046 |
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