Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function
Abstract Background Deoxynivalenol (DON) is a mycotoxin that has received recognition worldwide because of its ability to cause growth delay, nutrient malabsorption, weight loss, emesis, and a reduction of feed intake in livestock. Since DON-contaminated feedstuff is absorbed in the gastrointestinal...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40104-023-00976-4 |
| _version_ | 1797273638731251712 |
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| author | Tae Hong Kang Sang In Lee |
| author_facet | Tae Hong Kang Sang In Lee |
| author_sort | Tae Hong Kang |
| collection | DOAJ |
| description | Abstract Background Deoxynivalenol (DON) is a mycotoxin that has received recognition worldwide because of its ability to cause growth delay, nutrient malabsorption, weight loss, emesis, and a reduction of feed intake in livestock. Since DON-contaminated feedstuff is absorbed in the gastrointestinal tract, we used chicken organoids to assess the DON-induced dysfunction of the small intestine. Results We established a culture system using chicken organoids and characterized the organoids at passages 1 and 10. We confirmed the mRNA expression levels of various cell markers in the organoids, such as KI67, leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5), mucin 2 (MUC2), chromogranin A (CHGA), cytokeratin 19 (CK19), lysozyme (LYZ), and microtubule-associated doublecortin-like kinase 1 (DCLK1), and compared the results to those of the small intestine. Our results showed that the organoids displayed functional similarities in permeability compared to the small intestine. DON damaged the tight junctions of the organoids, which resulted in increased permeability. Conclusions Our organoid culture displayed topological, genetic, and functional similarities with the small intestine cells. Based on these similarities, we confirmed that DON causes small intestine dysfunction. Chicken organoids offer a practical model for the research of harmful substances. |
| first_indexed | 2024-03-07T14:47:16Z |
| format | Article |
| id | doaj.art-088bddc18a79418aa7967217d09088cc |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2024-03-07T14:47:16Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-088bddc18a79418aa7967217d09088cc2024-03-05T19:52:46ZengBMCJournal of Animal Science and Biotechnology2049-18912024-02-0115111210.1186/s40104-023-00976-4Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier functionTae Hong Kang0Sang In Lee1Department of Animal Science and Biotechnology, Kyungpook National UniversityDepartment of Animal Science and Biotechnology, Kyungpook National UniversityAbstract Background Deoxynivalenol (DON) is a mycotoxin that has received recognition worldwide because of its ability to cause growth delay, nutrient malabsorption, weight loss, emesis, and a reduction of feed intake in livestock. Since DON-contaminated feedstuff is absorbed in the gastrointestinal tract, we used chicken organoids to assess the DON-induced dysfunction of the small intestine. Results We established a culture system using chicken organoids and characterized the organoids at passages 1 and 10. We confirmed the mRNA expression levels of various cell markers in the organoids, such as KI67, leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5), mucin 2 (MUC2), chromogranin A (CHGA), cytokeratin 19 (CK19), lysozyme (LYZ), and microtubule-associated doublecortin-like kinase 1 (DCLK1), and compared the results to those of the small intestine. Our results showed that the organoids displayed functional similarities in permeability compared to the small intestine. DON damaged the tight junctions of the organoids, which resulted in increased permeability. Conclusions Our organoid culture displayed topological, genetic, and functional similarities with the small intestine cells. Based on these similarities, we confirmed that DON causes small intestine dysfunction. Chicken organoids offer a practical model for the research of harmful substances.https://doi.org/10.1186/s40104-023-00976-4Barrier functionDeoxynivalenolOrganoids |
| spellingShingle | Tae Hong Kang Sang In Lee Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function Journal of Animal Science and Biotechnology Barrier function Deoxynivalenol Organoids |
| title | Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function |
| title_full | Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function |
| title_fullStr | Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function |
| title_full_unstemmed | Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function |
| title_short | Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function |
| title_sort | establishment of a chicken intestinal organoid culture system to assess deoxynivalenol induced damage of the intestinal barrier function |
| topic | Barrier function Deoxynivalenol Organoids |
| url | https://doi.org/10.1186/s40104-023-00976-4 |
| work_keys_str_mv | AT taehongkang establishmentofachickenintestinalorganoidculturesystemtoassessdeoxynivalenolinduceddamageoftheintestinalbarrierfunction AT sanginlee establishmentofachickenintestinalorganoidculturesystemtoassessdeoxynivalenolinduceddamageoftheintestinalbarrierfunction |