Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis
Accurate and appropriate extrapulmonary tuberculosis (EPTB) diagnosis remains challenging due to its paucibacillary nature, requirement of invasive collection procedures, and lack of sensitive tests. This study investigated the diagnostic performance of different methods for the diagnosis of EPTB. A...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/1066 |
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| author | Mohammad Khaja Mafij Uddin Md. Fahim Ather Senjuti Kabir Arfatur Rahman Sabrina Choudhury Rumana Nasrin Tanjina Rahman S. M. Mazidur Rahman Shahriar Ahmed Sayera Banu |
| author_facet | Mohammad Khaja Mafij Uddin Md. Fahim Ather Senjuti Kabir Arfatur Rahman Sabrina Choudhury Rumana Nasrin Tanjina Rahman S. M. Mazidur Rahman Shahriar Ahmed Sayera Banu |
| author_sort | Mohammad Khaja Mafij Uddin |
| collection | DOAJ |
| description | Accurate and appropriate extrapulmonary tuberculosis (EPTB) diagnosis remains challenging due to its paucibacillary nature, requirement of invasive collection procedures, and lack of sensitive tests. This study investigated the diagnostic performance of different methods for the diagnosis of EPTB. A total of 1340 EPTB specimens were collected from presumptive EPTB patients from four different hospitals between November 2015 and March 2017. The collected specimens were tested with AFB microscopy, culture, Xpert MTB/RIF assay (Xpert), and MTBDR<i>plus</i> assay. Among the 1340 EPTB specimens, 49 (3.66%), 141 (10.52%), 166 (12.39%), and 154 (11.49%) were positive in AFB microscopy, culture, Xpert MTB/RIF, and MTBDR<i>plus</i> assay, respectively. A total of 194 (14.9%) cases were found positive in at least one of these methods. Using culture as a reference standard, the sensitivity and specificity of AFB microscopy, Xpert MTB/RIF, and MTBDR<i>plus</i> assay were: 27.0%/99.1%, 83.7%/96.0%, and 79.4%/96.5%, respectively. Compared to the composite reference standard, the sensitivity of culture, AFB microscopy, Xpert MTB/RIF, and MTBDR<i>plus</i> assay was 72.7%, 25.3%, 85.6%, and 79.4%, respectively, with a specificity of 100% for all the methods. The Xpert MTB/RIF assay showed the highest sensitivity compared to other methods. Considering the short turnaround time and promising findings, Xpert MTB/RIF assay should be integrated into national TB guidelines as a routine diagnostic test. |
| first_indexed | 2024-03-11T04:42:09Z |
| format | Article |
| id | doaj.art-088c49c2230c4f5fa3717bdc39d4a770 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:42:09Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-088c49c2230c4f5fa3717bdc39d4a7702023-11-17T20:34:31ZengMDPI AGMicroorganisms2076-26072023-04-01114106610.3390/microorganisms11041066Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary TuberculosisMohammad Khaja Mafij Uddin0Md. Fahim Ather1Senjuti Kabir2Arfatur Rahman3Sabrina Choudhury4Rumana Nasrin5Tanjina Rahman6S. M. Mazidur Rahman7Shahriar Ahmed8Sayera Banu9Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshInfectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, BangladeshAccurate and appropriate extrapulmonary tuberculosis (EPTB) diagnosis remains challenging due to its paucibacillary nature, requirement of invasive collection procedures, and lack of sensitive tests. This study investigated the diagnostic performance of different methods for the diagnosis of EPTB. A total of 1340 EPTB specimens were collected from presumptive EPTB patients from four different hospitals between November 2015 and March 2017. The collected specimens were tested with AFB microscopy, culture, Xpert MTB/RIF assay (Xpert), and MTBDR<i>plus</i> assay. Among the 1340 EPTB specimens, 49 (3.66%), 141 (10.52%), 166 (12.39%), and 154 (11.49%) were positive in AFB microscopy, culture, Xpert MTB/RIF, and MTBDR<i>plus</i> assay, respectively. A total of 194 (14.9%) cases were found positive in at least one of these methods. Using culture as a reference standard, the sensitivity and specificity of AFB microscopy, Xpert MTB/RIF, and MTBDR<i>plus</i> assay were: 27.0%/99.1%, 83.7%/96.0%, and 79.4%/96.5%, respectively. Compared to the composite reference standard, the sensitivity of culture, AFB microscopy, Xpert MTB/RIF, and MTBDR<i>plus</i> assay was 72.7%, 25.3%, 85.6%, and 79.4%, respectively, with a specificity of 100% for all the methods. The Xpert MTB/RIF assay showed the highest sensitivity compared to other methods. Considering the short turnaround time and promising findings, Xpert MTB/RIF assay should be integrated into national TB guidelines as a routine diagnostic test.https://www.mdpi.com/2076-2607/11/4/1066tuberculosisextrapulmonarydiagnosticGeneXpertsensitivityspecificity |
| spellingShingle | Mohammad Khaja Mafij Uddin Md. Fahim Ather Senjuti Kabir Arfatur Rahman Sabrina Choudhury Rumana Nasrin Tanjina Rahman S. M. Mazidur Rahman Shahriar Ahmed Sayera Banu Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis Microorganisms tuberculosis extrapulmonary diagnostic GeneXpert sensitivity specificity |
| title | Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis |
| title_full | Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis |
| title_fullStr | Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis |
| title_full_unstemmed | Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis |
| title_short | Diagnostic Performance of Different Laboratory Methods for the Detection of Extrapulmonary Tuberculosis |
| title_sort | diagnostic performance of different laboratory methods for the detection of extrapulmonary tuberculosis |
| topic | tuberculosis extrapulmonary diagnostic GeneXpert sensitivity specificity |
| url | https://www.mdpi.com/2076-2607/11/4/1066 |
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