High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis
Introduction: Nasal polyposis is a progressive inflammatory disease that reduces the quality of life. The role of apoptotic and autophagic pathways in nasal polyposis pathogenesis is not yet clearly known. Objective: In this study we aimed to investigate apoptotic (MAPK/JNK), anti-apoptotic (PI3K/mT...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Brazilian Journal of Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1808869420300069 |
| _version_ | 1819117969128554496 |
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| author | Fatma Simsek Erdem Eren Selen Bahceci Ibrahim Aladag |
| author_facet | Fatma Simsek Erdem Eren Selen Bahceci Ibrahim Aladag |
| author_sort | Fatma Simsek |
| collection | DOAJ |
| description | Introduction: Nasal polyposis is a progressive inflammatory disease that reduces the quality of life. The role of apoptotic and autophagic pathways in nasal polyposis pathogenesis is not yet clearly known. Objective: In this study we aimed to investigate apoptotic (MAPK/JNK), anti-apoptotic (PI3K/mTOR) and autophagic (LC3) pathways which are related each other in the nasal polyposis tissues. Methods: Twenty patients with nasal polyps and fifteen patients going through an inferior turbinate reduction were included in this study. Patients with asthma, Samter triad and allergic fungal sinusitis were excluded from the study. The apoptotic and autophagic pathways were investigated in paraffin-embedded nasal tissue sections of 20 NP and 15 samples from inferior turbinate reduction by H&E and immunohistochemistry with h-score. TUNEL method with apoptotic index was used to demonstrate apoptotic cells. Results: Decreased immunoreactivity of P38 MAPK (p < 0.005) and JNK (p < 0.005) were observed in nasal polyposis compared to material from inferior turbinate reduction. This decrease may indicate a downregulation of apoptosis as demonstrated by decreased TUNEL staining in nasal polyposis (p < 0.005). The PI3K (p < 0.002) and mTOR (p < 0.005) immunoreactivities were increased in nasal polyposis. This increase indicates a downregulation of autophagy as demonstrated by decreased LC3 staining in nasal polyposis (p < 0.001). Conclusion: Deficient apoptosis and autophagy through MAPK/JNK and PI3K/mTOR pathways may have a role in the pathogenesis of nasal polyposis. |
| first_indexed | 2024-12-22T05:41:25Z |
| format | Article |
| id | doaj.art-088d4bcef18446f09a470231dfde655d |
| institution | Directory Open Access Journal |
| issn | 1808-8694 |
| language | English |
| last_indexed | 2024-12-22T05:41:25Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Otorhinolaryngology |
| spelling | doaj.art-088d4bcef18446f09a470231dfde655d2022-12-21T18:37:10ZengElsevierBrazilian Journal of Otorhinolaryngology1808-86942021-09-01875572577High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposisFatma Simsek0Erdem Eren1Selen Bahceci2Ibrahim Aladag3Izmir Katip Celebi University, Department of Histology and Embryology, Izmir, Turkey; Corresponding author.Izmir Katip Celebi University, Atatürk Training and Research Hospital, Department of Otorhinolaryngology, Izmir, TurkeyIzmir Katip Celebi University, Department of Histology and Embryology, Izmir, TurkeyIzmir Katip Celebi University, Atatürk Training and Research Hospital, Department of Otorhinolaryngology, Izmir, TurkeyIntroduction: Nasal polyposis is a progressive inflammatory disease that reduces the quality of life. The role of apoptotic and autophagic pathways in nasal polyposis pathogenesis is not yet clearly known. Objective: In this study we aimed to investigate apoptotic (MAPK/JNK), anti-apoptotic (PI3K/mTOR) and autophagic (LC3) pathways which are related each other in the nasal polyposis tissues. Methods: Twenty patients with nasal polyps and fifteen patients going through an inferior turbinate reduction were included in this study. Patients with asthma, Samter triad and allergic fungal sinusitis were excluded from the study. The apoptotic and autophagic pathways were investigated in paraffin-embedded nasal tissue sections of 20 NP and 15 samples from inferior turbinate reduction by H&E and immunohistochemistry with h-score. TUNEL method with apoptotic index was used to demonstrate apoptotic cells. Results: Decreased immunoreactivity of P38 MAPK (p < 0.005) and JNK (p < 0.005) were observed in nasal polyposis compared to material from inferior turbinate reduction. This decrease may indicate a downregulation of apoptosis as demonstrated by decreased TUNEL staining in nasal polyposis (p < 0.005). The PI3K (p < 0.002) and mTOR (p < 0.005) immunoreactivities were increased in nasal polyposis. This increase indicates a downregulation of autophagy as demonstrated by decreased LC3 staining in nasal polyposis (p < 0.001). Conclusion: Deficient apoptosis and autophagy through MAPK/JNK and PI3K/mTOR pathways may have a role in the pathogenesis of nasal polyposis.http://www.sciencedirect.com/science/article/pii/S1808869420300069Nasal polypApoptosisAutophagyTUNELEosinophil |
| spellingShingle | Fatma Simsek Erdem Eren Selen Bahceci Ibrahim Aladag High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis Brazilian Journal of Otorhinolaryngology Nasal polyp Apoptosis Autophagy TUNEL Eosinophil |
| title | High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis |
| title_full | High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis |
| title_fullStr | High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis |
| title_full_unstemmed | High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis |
| title_short | High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis |
| title_sort | high pi3k mtor and low mapk jnk activity results in decreased apoptosis and autophagy in nasal polyposis |
| topic | Nasal polyp Apoptosis Autophagy TUNEL Eosinophil |
| url | http://www.sciencedirect.com/science/article/pii/S1808869420300069 |
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