The potential role of pumpkin seeds oil on methotrexate-induced lung toxicity
Abstract Many chemotherapeutic drugs cause adverse pulmonary reactions leading to severe pulmonary disease. Though methotrexate (MTX) is used for the treatment of cancer and other diseases, it is highly toxic with multiple adverse effects including pulmonary toxicity. Essential oils represent an ope...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34143-6 |
| _version_ | 1797832133790662656 |
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| author | Aya M. Abosrea Heba S. Aboul Ezz Sahar M. Mahmoud Mohamed R. Mousa Nawal A. Ahmed |
| author_facet | Aya M. Abosrea Heba S. Aboul Ezz Sahar M. Mahmoud Mohamed R. Mousa Nawal A. Ahmed |
| author_sort | Aya M. Abosrea |
| collection | DOAJ |
| description | Abstract Many chemotherapeutic drugs cause adverse pulmonary reactions leading to severe pulmonary disease. Though methotrexate (MTX) is used for the treatment of cancer and other diseases, it is highly toxic with multiple adverse effects including pulmonary toxicity. Essential oils represent an open frontier for pharmaceutical sciences due to their wide range of pharmacological properties. Pumpkin seeds oil (PSO) was used to investigate its ability to alleviate methotrexate-induced lung toxicity in rats. Lung tissue from MTX-treated group revealed a decrease in malondialdehyde, glutathione, and nitric oxide accompanied by a marked inhibition in cholinesterase activity, and enhanced catalase activity, tumor necrosis factor-α, interleukin-6 and vascular endothelial growth factor levels. Analysis of PSO revealed that the oil was rich in hexadecanoic acid, decane methyl esters, squalene, polydecane, docosane, and other derivatives. Administration of PSO ameliorated the oxidant/antioxidant and proinflammatory changes induced by MTX in the lung tissue. Histological examinations confirmed the potency of PSO in reducing the histopathological alterations induced by MTX. Immunohistochemical analysis showed decreased nuclear factor-kappa B and caspase 3 expression after PSO. The present data indicated the protective efficiency of PSO against MTX-induced lung injury by decreasing oxidative damage, inflammation and apoptosis and could thus be recommended as an adjuvant therapy. |
| first_indexed | 2024-04-09T14:02:53Z |
| format | Article |
| id | doaj.art-088ef45429ed427eb870d6d1ea3c3749 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T14:02:53Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-088ef45429ed427eb870d6d1ea3c37492023-05-07T11:10:38ZengNature PortfolioScientific Reports2045-23222023-05-0113111510.1038/s41598-023-34143-6The potential role of pumpkin seeds oil on methotrexate-induced lung toxicityAya M. Abosrea0Heba S. Aboul Ezz1Sahar M. Mahmoud2Mohamed R. Mousa3Nawal A. Ahmed4Department of Zoology, Faculty of Science, Cairo UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityDepartment of Pathology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityAbstract Many chemotherapeutic drugs cause adverse pulmonary reactions leading to severe pulmonary disease. Though methotrexate (MTX) is used for the treatment of cancer and other diseases, it is highly toxic with multiple adverse effects including pulmonary toxicity. Essential oils represent an open frontier for pharmaceutical sciences due to their wide range of pharmacological properties. Pumpkin seeds oil (PSO) was used to investigate its ability to alleviate methotrexate-induced lung toxicity in rats. Lung tissue from MTX-treated group revealed a decrease in malondialdehyde, glutathione, and nitric oxide accompanied by a marked inhibition in cholinesterase activity, and enhanced catalase activity, tumor necrosis factor-α, interleukin-6 and vascular endothelial growth factor levels. Analysis of PSO revealed that the oil was rich in hexadecanoic acid, decane methyl esters, squalene, polydecane, docosane, and other derivatives. Administration of PSO ameliorated the oxidant/antioxidant and proinflammatory changes induced by MTX in the lung tissue. Histological examinations confirmed the potency of PSO in reducing the histopathological alterations induced by MTX. Immunohistochemical analysis showed decreased nuclear factor-kappa B and caspase 3 expression after PSO. The present data indicated the protective efficiency of PSO against MTX-induced lung injury by decreasing oxidative damage, inflammation and apoptosis and could thus be recommended as an adjuvant therapy.https://doi.org/10.1038/s41598-023-34143-6 |
| spellingShingle | Aya M. Abosrea Heba S. Aboul Ezz Sahar M. Mahmoud Mohamed R. Mousa Nawal A. Ahmed The potential role of pumpkin seeds oil on methotrexate-induced lung toxicity Scientific Reports |
| title | The potential role of pumpkin seeds oil on methotrexate-induced lung toxicity |
| title_full | The potential role of pumpkin seeds oil on methotrexate-induced lung toxicity |
| title_fullStr | The potential role of pumpkin seeds oil on methotrexate-induced lung toxicity |
| title_full_unstemmed | The potential role of pumpkin seeds oil on methotrexate-induced lung toxicity |
| title_short | The potential role of pumpkin seeds oil on methotrexate-induced lung toxicity |
| title_sort | potential role of pumpkin seeds oil on methotrexate induced lung toxicity |
| url | https://doi.org/10.1038/s41598-023-34143-6 |
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