Propofol-induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamus
Propofol is the most widely used intravenous general anesthetic; however, the neuronal circuits that mediate its anesthetic effects are still poorly understood. Glutamatergic neurons in the lateral hypothalamus have been reported to be involved in maintenance of arousal and consciousness. Using Vglu...
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| Format: | Article |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2024.1327293/full |
| _version_ | 1797356927475253248 |
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| author | Yan Huang Yong Xiao Linji Li Xinglong Feng Weixing Ding Feng Cai |
| author_facet | Yan Huang Yong Xiao Linji Li Xinglong Feng Weixing Ding Feng Cai |
| author_sort | Yan Huang |
| collection | DOAJ |
| description | Propofol is the most widely used intravenous general anesthetic; however, the neuronal circuits that mediate its anesthetic effects are still poorly understood. Glutamatergic neurons in the lateral hypothalamus have been reported to be involved in maintenance of arousal and consciousness. Using Vglut2-Cre transgenic mice, we recorded this group of cells specifically and found that propofol can directly inhibit the glutamatergic neurons, and enhance inhibitory synaptic inputs on these cells, thereby reducing neuronal excitability. Through chemogenetic interventions, we found that inhibition of these neurons increased the duration of propofol-induced anesthesia and reduced movement in the animals after the recovery of right reflex. In contrast, activating this group of cells reduced the duration of propofol anesthesia and increased the animals’ locomotor activity after the recovery of right reflex. These results suggest that propofol-induced anesthesia involves the inhibition of glutamatergic neurons in the lateral hypothalamus. |
| first_indexed | 2024-03-08T14:36:53Z |
| format | Article |
| id | doaj.art-088f93001aca4f5f8b95707c9734d13c |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-03-08T14:36:53Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-088f93001aca4f5f8b95707c9734d13c2024-01-12T04:20:03ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2024-01-011810.3389/fnins.2024.13272931327293Propofol-induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamusYan Huang0Yong Xiao1Linji Li2Xinglong Feng3Weixing Ding4Feng Cai5Department of Anesthesiology, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College, Nanchong, ChinaEmergency Department of the General Hospital of the Tibet Military Region, Lhasa, ChinaDepartment of Anesthesiology, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College, Nanchong, ChinaDepartment of Anesthesiology, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College, Nanchong, ChinaQujing Secend Peopie’s Hospital, Department of Pain, Qujing, Yunnan, ChinaDepartment of Urologyand Neurocardiothoracic Surgery, 927 Hospital of the Joint Logistics Support Force of the Chinese People’s LiberationArmy, Puer, ChinaPropofol is the most widely used intravenous general anesthetic; however, the neuronal circuits that mediate its anesthetic effects are still poorly understood. Glutamatergic neurons in the lateral hypothalamus have been reported to be involved in maintenance of arousal and consciousness. Using Vglut2-Cre transgenic mice, we recorded this group of cells specifically and found that propofol can directly inhibit the glutamatergic neurons, and enhance inhibitory synaptic inputs on these cells, thereby reducing neuronal excitability. Through chemogenetic interventions, we found that inhibition of these neurons increased the duration of propofol-induced anesthesia and reduced movement in the animals after the recovery of right reflex. In contrast, activating this group of cells reduced the duration of propofol anesthesia and increased the animals’ locomotor activity after the recovery of right reflex. These results suggest that propofol-induced anesthesia involves the inhibition of glutamatergic neurons in the lateral hypothalamus.https://www.frontiersin.org/articles/10.3389/fnins.2024.1327293/fullanesthesiapropofollateral hypothalamusglutamatergic neuronshM4Di receptors |
| spellingShingle | Yan Huang Yong Xiao Linji Li Xinglong Feng Weixing Ding Feng Cai Propofol-induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamus Frontiers in Neuroscience anesthesia propofol lateral hypothalamus glutamatergic neurons hM4Di receptors |
| title | Propofol-induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamus |
| title_full | Propofol-induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamus |
| title_fullStr | Propofol-induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamus |
| title_full_unstemmed | Propofol-induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamus |
| title_short | Propofol-induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamus |
| title_sort | propofol induced anesthesia involves the direct inhibition of glutamatergic neurons in the lateral hypothalamus |
| topic | anesthesia propofol lateral hypothalamus glutamatergic neurons hM4Di receptors |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2024.1327293/full |
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