Molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian Enterocytozoon hepatopenaei (EHP)
Abstract Microsporidia are obligate intracellular parasites that lost several enzymes required in energy production. The expansion of transporter families in these organisms enables them to hijack ATP from hosts. In this study, nucleotide transporters of the microsporidian Enterocytozoon hepatopenae...
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47114-8 |
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| author | Orawan Thepmanee Natthinee Munkongwongsiri Anuphap Prachumwat Vanvimon Saksmerprome Sarocha Jitrakorn Kallaya Sritunyalucksana Rapeepun Vanichviriyakit Sittinan Chanarat Pattana Jaroenlak Ornchuma Itsathitphaisarn |
| author_facet | Orawan Thepmanee Natthinee Munkongwongsiri Anuphap Prachumwat Vanvimon Saksmerprome Sarocha Jitrakorn Kallaya Sritunyalucksana Rapeepun Vanichviriyakit Sittinan Chanarat Pattana Jaroenlak Ornchuma Itsathitphaisarn |
| author_sort | Orawan Thepmanee |
| collection | DOAJ |
| description | Abstract Microsporidia are obligate intracellular parasites that lost several enzymes required in energy production. The expansion of transporter families in these organisms enables them to hijack ATP from hosts. In this study, nucleotide transporters of the microsporidian Enterocytozoon hepatopenaei (EHP), which causes slow growth in economically valuable Penaeus shrimp, were characterized. Analysis of the EHP genome suggested the presence of four putative nucleotide transporter genes, namely EhNTT1, EhNTT2, EhNTT3, and EhNTT4. Sequence alignment revealed four charged amino acids that are conserved in previously characterized nucleotide transporters. Phylogenetic analysis suggested that EhNTT1, 3, and 4 were derived from one horizontal gene transfer event, which was independent from that of EhNTT2. Localization of EhNTT1 and EhNTT2 using immunofluorescence analysis revealed positive signals within the envelope of developing plasmodia and on mature spores. Knockdown of EhNTT2 by double administration of sequence specific double-stranded RNA resulted in a significant reduction in EHP copy numbers, suggesting that EhNTT2 is crucial for EHP replication in shrimp. Taken together, the insight into the roles of NTTs in microsporidian proliferation can provide the biological basis for the development of alternative control strategies for microsporidian infection in shrimp. |
| first_indexed | 2024-03-10T17:46:30Z |
| format | Article |
| id | doaj.art-088f9ad1189a4faa986e357f17094ae2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:46:30Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-088f9ad1189a4faa986e357f17094ae22023-11-20T09:30:44ZengNature PortfolioScientific Reports2045-23222023-11-0113111810.1038/s41598-023-47114-8Molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian Enterocytozoon hepatopenaei (EHP)Orawan Thepmanee0Natthinee Munkongwongsiri1Anuphap Prachumwat2Vanvimon Saksmerprome3Sarocha Jitrakorn4Kallaya Sritunyalucksana5Rapeepun Vanichviriyakit6Sittinan Chanarat7Pattana Jaroenlak8Ornchuma Itsathitphaisarn9Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol UniversityNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol UniversityCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol UniversityCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol UniversityNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol UniversityDepartment of Biochemistry, Faculty of Science, Mahidol UniversityCenter of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn UniversityCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol UniversityAbstract Microsporidia are obligate intracellular parasites that lost several enzymes required in energy production. The expansion of transporter families in these organisms enables them to hijack ATP from hosts. In this study, nucleotide transporters of the microsporidian Enterocytozoon hepatopenaei (EHP), which causes slow growth in economically valuable Penaeus shrimp, were characterized. Analysis of the EHP genome suggested the presence of four putative nucleotide transporter genes, namely EhNTT1, EhNTT2, EhNTT3, and EhNTT4. Sequence alignment revealed four charged amino acids that are conserved in previously characterized nucleotide transporters. Phylogenetic analysis suggested that EhNTT1, 3, and 4 were derived from one horizontal gene transfer event, which was independent from that of EhNTT2. Localization of EhNTT1 and EhNTT2 using immunofluorescence analysis revealed positive signals within the envelope of developing plasmodia and on mature spores. Knockdown of EhNTT2 by double administration of sequence specific double-stranded RNA resulted in a significant reduction in EHP copy numbers, suggesting that EhNTT2 is crucial for EHP replication in shrimp. Taken together, the insight into the roles of NTTs in microsporidian proliferation can provide the biological basis for the development of alternative control strategies for microsporidian infection in shrimp.https://doi.org/10.1038/s41598-023-47114-8 |
| spellingShingle | Orawan Thepmanee Natthinee Munkongwongsiri Anuphap Prachumwat Vanvimon Saksmerprome Sarocha Jitrakorn Kallaya Sritunyalucksana Rapeepun Vanichviriyakit Sittinan Chanarat Pattana Jaroenlak Ornchuma Itsathitphaisarn Molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian Enterocytozoon hepatopenaei (EHP) Scientific Reports |
| title | Molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian Enterocytozoon hepatopenaei (EHP) |
| title_full | Molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian Enterocytozoon hepatopenaei (EHP) |
| title_fullStr | Molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian Enterocytozoon hepatopenaei (EHP) |
| title_full_unstemmed | Molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian Enterocytozoon hepatopenaei (EHP) |
| title_short | Molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian Enterocytozoon hepatopenaei (EHP) |
| title_sort | molecular and cellular characterization of four putative nucleotide transporters from the shrimp microsporidian enterocytozoon hepatopenaei ehp |
| url | https://doi.org/10.1038/s41598-023-47114-8 |
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