Postharvest control of Rhizopus stolonifer on blackberry (Rubus fruticosus) by blackberry native crop bacteria
The potential of four native bacterial strains of blackberries cv. Brazos (Rubus fruticosus): Bacillus subtilis (BSS), Bacillus subtilis (BSL), Bacillus licheniformis (BLI) and Leifsonia aquatica (LAQ), was evaluated for the postharvest control of soft rot caused by Rhizopus stolonifer in blackberr...
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| Format: | Article |
| Language: | English |
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Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo
2019-12-01
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| Series: | Revista de la Facultad de Ciencias Agrarias |
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| Online Access: | https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/2703 |
| _version_ | 1797755992043159552 |
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| author | Ismael F. Chavez-Diaz Hortencia G. Mena-Violante Ana N. Hernandez-Lauzardo Guadalupe Oyoque-Salcedo Ernesto Oregel-Zamudio M. Valentina Angoa-Perez |
| author_facet | Ismael F. Chavez-Diaz Hortencia G. Mena-Violante Ana N. Hernandez-Lauzardo Guadalupe Oyoque-Salcedo Ernesto Oregel-Zamudio M. Valentina Angoa-Perez |
| author_sort | Ismael F. Chavez-Diaz |
| collection | DOAJ |
| description |
The potential of four native bacterial strains of blackberries cv. Brazos (Rubus fruticosus): Bacillus subtilis (BSS), Bacillus subtilis (BSL), Bacillus licheniformis (BLI) and Leifsonia aquatica (LAQ), was evaluated for the postharvest control of soft rot caused by Rhizopus stolonifer in blackberry fruits. The fruits were treated with cell suspensions (CS) and cell-free supernatants (CFE) from each bacterial strain and were infected with two strains of R. stolonifer (RSA and RSC). The severity and inhibition percentage of the disease were determined. Additionally, the inhibition by siderophores and the inhibition percentage of R. stolonifer spore germination were analyzed as possible control mechanisms. The CS of BSS inhibited RSA by 45.8%, followed by CFE of LAQ which controlled the same strain by 39.7%. The CS of BLI inhibited RSC by 37.7%, whereas the CFE of BSS and LAQ controlled it by 47.7 and 41.8%, respectively. All bacterial strains inhibited RSA and RSC by siderophores production (38.7 to 48.6 %) and the inhibition of spore germination of RSC was higher than 93% after 48 h. This work is one of the first to report R. stolonifer control by native bacteria CS and CFE, particularly LAQ in postharvested blackberry fruits. These results show the combination of mechanisms used by bacteria to control both R. stolonifer strains.
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| first_indexed | 2024-03-12T17:54:10Z |
| format | Article |
| id | doaj.art-088faef4f6b84be0bc95c02a4fc99f23 |
| institution | Directory Open Access Journal |
| issn | 0370-4661 1853-8665 |
| language | English |
| last_indexed | 2024-03-12T17:54:10Z |
| publishDate | 2019-12-01 |
| publisher | Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo |
| record_format | Article |
| series | Revista de la Facultad de Ciencias Agrarias |
| spelling | doaj.art-088faef4f6b84be0bc95c02a4fc99f232023-08-02T18:01:24ZengFacultad de Ciencias Agrarias. Universidad Nacional de CuyoRevista de la Facultad de Ciencias Agrarias0370-46611853-86652019-12-01512Postharvest control of Rhizopus stolonifer on blackberry (Rubus fruticosus) by blackberry native crop bacteriaIsmael F. Chavez-Diaz0Hortencia G. Mena-Violante1Ana N. Hernandez-Lauzardo2Guadalupe Oyoque-Salcedo3Ernesto Oregel-Zamudio4M. Valentina Angoa-Perez5Instituto Politécnico Nacional. CIIDIR IPN Unidad Michoacán. Justo Sierra 28. Centro. C.P. 59510. Jiquilpan. Michoacán. México.Instituto Politécnico Nacional. CIIDIR IPN Unidad Michoacán. Justo Sierra 28. Centro. C.P. 59510. Jiquilpan. Michoacán. México.Instituto Politécnico Nacional. CEPROBI. Departamento de Interacciones PlantaInsecto Carretera Yautepec-Jojutla. Km.6. Calle CEPROBI No. 8. Col. San Isidro. C.P. 62731. Apartado Postal 24. Yautepec. Morelos. Mexico.Instituto Politécnico Nacional. CIIDIR IPN Unidad Michoacán. Justo Sierra 28. Centro. C.P. 59510. Jiquilpan. Michoacán. México. Instituto Politécnico Nacional. CIIDIR IPN Unidad Michoacán. Justo Sierra 28. Centro. C.P. 59510. Jiquilpan. Michoacán. México. Instituto Politécnico Nacional. CIIDIR IPN Unidad Michoacán. Justo Sierra 28. Centro. C.P. 59510. Jiquilpan. Michoacán. México. The potential of four native bacterial strains of blackberries cv. Brazos (Rubus fruticosus): Bacillus subtilis (BSS), Bacillus subtilis (BSL), Bacillus licheniformis (BLI) and Leifsonia aquatica (LAQ), was evaluated for the postharvest control of soft rot caused by Rhizopus stolonifer in blackberry fruits. The fruits were treated with cell suspensions (CS) and cell-free supernatants (CFE) from each bacterial strain and were infected with two strains of R. stolonifer (RSA and RSC). The severity and inhibition percentage of the disease were determined. Additionally, the inhibition by siderophores and the inhibition percentage of R. stolonifer spore germination were analyzed as possible control mechanisms. The CS of BSS inhibited RSA by 45.8%, followed by CFE of LAQ which controlled the same strain by 39.7%. The CS of BLI inhibited RSC by 37.7%, whereas the CFE of BSS and LAQ controlled it by 47.7 and 41.8%, respectively. All bacterial strains inhibited RSA and RSC by siderophores production (38.7 to 48.6 %) and the inhibition of spore germination of RSC was higher than 93% after 48 h. This work is one of the first to report R. stolonifer control by native bacteria CS and CFE, particularly LAQ in postharvested blackberry fruits. These results show the combination of mechanisms used by bacteria to control both R. stolonifer strains. https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/2703Postharvest berriesBiocontrolCell-free ExtractLeifsoniaBacillusRhizopus stolonifer |
| spellingShingle | Ismael F. Chavez-Diaz Hortencia G. Mena-Violante Ana N. Hernandez-Lauzardo Guadalupe Oyoque-Salcedo Ernesto Oregel-Zamudio M. Valentina Angoa-Perez Postharvest control of Rhizopus stolonifer on blackberry (Rubus fruticosus) by blackberry native crop bacteria Revista de la Facultad de Ciencias Agrarias Postharvest berries Biocontrol Cell-free Extract Leifsonia Bacillus Rhizopus stolonifer |
| title | Postharvest control of Rhizopus stolonifer on blackberry (Rubus fruticosus) by blackberry native crop bacteria |
| title_full | Postharvest control of Rhizopus stolonifer on blackberry (Rubus fruticosus) by blackberry native crop bacteria |
| title_fullStr | Postharvest control of Rhizopus stolonifer on blackberry (Rubus fruticosus) by blackberry native crop bacteria |
| title_full_unstemmed | Postharvest control of Rhizopus stolonifer on blackberry (Rubus fruticosus) by blackberry native crop bacteria |
| title_short | Postharvest control of Rhizopus stolonifer on blackberry (Rubus fruticosus) by blackberry native crop bacteria |
| title_sort | postharvest control of rhizopus stolonifer on blackberry rubus fruticosus by blackberry native crop bacteria |
| topic | Postharvest berries Biocontrol Cell-free Extract Leifsonia Bacillus Rhizopus stolonifer |
| url | https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/2703 |
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