Garcinol Alone and in Combination With Cisplatin Affect Cellular Behavior and PI3K/AKT Protein Phosphorylation in Human Ovarian Cancer Cells
Garcinol is a plant-derived compound that has some physiological benefits to human cells. However, the effect of garcinol on ovarian cancer (OC) cell proliferation and apoptosis is unknown. The current study aimed to examine the effects of garcinol alone and in combination with cisplatin (DDP) on ce...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-05-01
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| Series: | Dose-Response |
| Online Access: | https://doi.org/10.1177/1559325820926732 |
| _version_ | 1828880877919141888 |
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| author | Jie Zhang Huan Fang Jinguo Zhang Wencai Guan Guoxiong Xu |
| author_facet | Jie Zhang Huan Fang Jinguo Zhang Wencai Guan Guoxiong Xu |
| author_sort | Jie Zhang |
| collection | DOAJ |
| description | Garcinol is a plant-derived compound that has some physiological benefits to human cells. However, the effect of garcinol on ovarian cancer (OC) cell proliferation and apoptosis is unknown. The current study aimed to examine the effects of garcinol alone and in combination with cisplatin (DDP) on cellular behavior and to explore the expression pattern of PI3K/AKT and nuclear factor-κB (NF-κB) in human OC cells. We found that OVCAR-3 cell viability was decreased after garcinol treatment. Garcinol alone and in combination with DDP significantly inhibited cell proliferation and had a synergistic effect evaluated by CompuSyn software. The cell cycle analysis showed the S phase arrest by garcinol. Furthermore, garcinol alone and in combination with DDP promoted cell apoptosis. The garcinol-induced apoptosis was further confirmed by the detection of cleavage forms of PARP and caspase 3. An increase in proapoptotic factor Bax expression was also found in garcinol-treated cells. Moreover, garcinol significantly decreased the phosphorylation of PI3K and AKT proteins and downregulated the expression of NF-κB. Thus, our data demonstrated that garcinol has the potential to be used as an anticancer agent and may synergize the effect of DDP. These actions are most likely through the regulation of the PI3K/AKT and NF-κB pathways. |
| first_indexed | 2024-12-13T09:56:04Z |
| format | Article |
| id | doaj.art-08905695e4f44d7c89363ae24a7dfe34 |
| institution | Directory Open Access Journal |
| issn | 1559-3258 |
| language | English |
| last_indexed | 2024-12-13T09:56:04Z |
| publishDate | 2020-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Dose-Response |
| spelling | doaj.art-08905695e4f44d7c89363ae24a7dfe342022-12-21T23:51:46ZengSAGE PublishingDose-Response1559-32582020-05-011810.1177/1559325820926732Garcinol Alone and in Combination With Cisplatin Affect Cellular Behavior and PI3K/AKT Protein Phosphorylation in Human Ovarian Cancer CellsJie Zhang0Huan Fang1Jinguo Zhang2Wencai Guan3Guoxiong Xu4 Department of Clinical Nutrition, Jinshan Hospital, Fudan University, Shanghai, China Department of Clinical Pharmacy, Jinshan Hospital, Fudan University, Shanghai, China Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai, China Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai, China Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai, ChinaGarcinol is a plant-derived compound that has some physiological benefits to human cells. However, the effect of garcinol on ovarian cancer (OC) cell proliferation and apoptosis is unknown. The current study aimed to examine the effects of garcinol alone and in combination with cisplatin (DDP) on cellular behavior and to explore the expression pattern of PI3K/AKT and nuclear factor-κB (NF-κB) in human OC cells. We found that OVCAR-3 cell viability was decreased after garcinol treatment. Garcinol alone and in combination with DDP significantly inhibited cell proliferation and had a synergistic effect evaluated by CompuSyn software. The cell cycle analysis showed the S phase arrest by garcinol. Furthermore, garcinol alone and in combination with DDP promoted cell apoptosis. The garcinol-induced apoptosis was further confirmed by the detection of cleavage forms of PARP and caspase 3. An increase in proapoptotic factor Bax expression was also found in garcinol-treated cells. Moreover, garcinol significantly decreased the phosphorylation of PI3K and AKT proteins and downregulated the expression of NF-κB. Thus, our data demonstrated that garcinol has the potential to be used as an anticancer agent and may synergize the effect of DDP. These actions are most likely through the regulation of the PI3K/AKT and NF-κB pathways.https://doi.org/10.1177/1559325820926732 |
| spellingShingle | Jie Zhang Huan Fang Jinguo Zhang Wencai Guan Guoxiong Xu Garcinol Alone and in Combination With Cisplatin Affect Cellular Behavior and PI3K/AKT Protein Phosphorylation in Human Ovarian Cancer Cells Dose-Response |
| title | Garcinol Alone and in Combination With Cisplatin Affect Cellular Behavior and PI3K/AKT Protein Phosphorylation in Human Ovarian Cancer Cells |
| title_full | Garcinol Alone and in Combination With Cisplatin Affect Cellular Behavior and PI3K/AKT Protein Phosphorylation in Human Ovarian Cancer Cells |
| title_fullStr | Garcinol Alone and in Combination With Cisplatin Affect Cellular Behavior and PI3K/AKT Protein Phosphorylation in Human Ovarian Cancer Cells |
| title_full_unstemmed | Garcinol Alone and in Combination With Cisplatin Affect Cellular Behavior and PI3K/AKT Protein Phosphorylation in Human Ovarian Cancer Cells |
| title_short | Garcinol Alone and in Combination With Cisplatin Affect Cellular Behavior and PI3K/AKT Protein Phosphorylation in Human Ovarian Cancer Cells |
| title_sort | garcinol alone and in combination with cisplatin affect cellular behavior and pi3k akt protein phosphorylation in human ovarian cancer cells |
| url | https://doi.org/10.1177/1559325820926732 |
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