Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability
Chitosan-silica materials offer a specific environment for the adsorption of biofunctional molecules, such as the antimicrobial peptide KR-12. The objective here is to rationalize the changes in the physicochemical properties of these chitosan-silica materials in function of the synthesis pH, using...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Journal of Materials Research and Technology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2238785419310907 |
| _version_ | 1819010483537051648 |
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| author | Johnatan Diosa Fanny Guzman Claudia Bernal Monica Mesa |
| author_facet | Johnatan Diosa Fanny Guzman Claudia Bernal Monica Mesa |
| author_sort | Johnatan Diosa |
| collection | DOAJ |
| description | Chitosan-silica materials offer a specific environment for the adsorption of biofunctional molecules, such as the antimicrobial peptide KR-12. The objective here is to rationalize the changes in the physicochemical properties of these chitosan-silica materials in function of the synthesis pH, using 0.02 w/v % chitosan as catalyst and aggregation agent and, to correlate these characteristics with the loading/delivery and activity/stability of KR-12 antimicrobial peptide. The CS-6 material, prepared at pH 6, exhibits higher surface area (745 m2/g) and total pore volume (0.58 cm3/g) due to the lower incorporation of chitosan swollen chains (9.36 wt %). Higher pHs produced denser materials (CS-7 and CS-8) with higher entangled chitosan incorporated (> 17 wt%). Adsorption of KR-12 peptide was found to take two different mechanisms depending on the chitosan-silica support, Langmuir-type for CS-6 material and Freundlich-type for CS-7 and CS-8 materials. According to the KR-12 release profiles, more hydrophobic interactions were observed in the CS-6 material exposing Lys and Arg residues from the peptide towards the material surface. This was correlated with the higher antimicrobial activity of this material against S. aureus strain (MIC = 128 μg/mL). Additionally, the KR-12 peptide adsorbed in the CS-6 hybrid support is 34 % more protected from the proteolytic action of α-chymotrypsin than the free one. In conclusion, the proposed models of different porous environments in the studied chitosan-silica materials supports the KR-12 peptide loading/delivery mechanisms, leading to biofunctionalized solid antimicrobial materials exhibiting proteolytic stability. This knowledge is useful for designing new antibacterial materials for biomedical applications. Keywords: Chitosan-silica particle mechanism formation, KR-12 peptide adsorption/delivery mechanisms, KR-12 controlled release, KR-12/chitosan-silica antimicrobial solid materials, Proteolysis stability |
| first_indexed | 2024-12-21T01:12:59Z |
| format | Article |
| id | doaj.art-0890c180564a4e03b1116f34066b17a9 |
| institution | Directory Open Access Journal |
| issn | 2238-7854 |
| language | English |
| last_indexed | 2024-12-21T01:12:59Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Materials Research and Technology |
| spelling | doaj.art-0890c180564a4e03b1116f34066b17a92022-12-21T19:20:53ZengElsevierJournal of Materials Research and Technology2238-78542020-01-0191890901Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stabilityJohnatan Diosa0Fanny Guzman1Claudia Bernal2Monica Mesa3Grupo Ciencia de los Materiales, Instituto de Química, FCEN, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, ColombiaLaboratorio de Síntesis de Péptidos, Núcleo de Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, ChileInstituto de Investigación Multidisciplinario en Ciencia y Tecnología, Universidad de La Serena, Raul Bitran 1305, La Serena, Chile; Tecnología Enzimática para Bioprocesos, Departamento de Ingeniería de Alimentos, Universidad de La Serena, Raul Bitran 1305, La Serena, ChileGrupo Ciencia de los Materiales, Instituto de Química, FCEN, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia; Corresponding author.Chitosan-silica materials offer a specific environment for the adsorption of biofunctional molecules, such as the antimicrobial peptide KR-12. The objective here is to rationalize the changes in the physicochemical properties of these chitosan-silica materials in function of the synthesis pH, using 0.02 w/v % chitosan as catalyst and aggregation agent and, to correlate these characteristics with the loading/delivery and activity/stability of KR-12 antimicrobial peptide. The CS-6 material, prepared at pH 6, exhibits higher surface area (745 m2/g) and total pore volume (0.58 cm3/g) due to the lower incorporation of chitosan swollen chains (9.36 wt %). Higher pHs produced denser materials (CS-7 and CS-8) with higher entangled chitosan incorporated (> 17 wt%). Adsorption of KR-12 peptide was found to take two different mechanisms depending on the chitosan-silica support, Langmuir-type for CS-6 material and Freundlich-type for CS-7 and CS-8 materials. According to the KR-12 release profiles, more hydrophobic interactions were observed in the CS-6 material exposing Lys and Arg residues from the peptide towards the material surface. This was correlated with the higher antimicrobial activity of this material against S. aureus strain (MIC = 128 μg/mL). Additionally, the KR-12 peptide adsorbed in the CS-6 hybrid support is 34 % more protected from the proteolytic action of α-chymotrypsin than the free one. In conclusion, the proposed models of different porous environments in the studied chitosan-silica materials supports the KR-12 peptide loading/delivery mechanisms, leading to biofunctionalized solid antimicrobial materials exhibiting proteolytic stability. This knowledge is useful for designing new antibacterial materials for biomedical applications. Keywords: Chitosan-silica particle mechanism formation, KR-12 peptide adsorption/delivery mechanisms, KR-12 controlled release, KR-12/chitosan-silica antimicrobial solid materials, Proteolysis stabilityhttp://www.sciencedirect.com/science/article/pii/S2238785419310907 |
| spellingShingle | Johnatan Diosa Fanny Guzman Claudia Bernal Monica Mesa Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability Journal of Materials Research and Technology |
| title | Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability |
| title_full | Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability |
| title_fullStr | Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability |
| title_full_unstemmed | Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability |
| title_short | Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability |
| title_sort | formation mechanisms of chitosan silica hybrid materials and its performance as solid support for kr 12 peptide adsorption impact on kr 12 antimicrobial activity and proteolytic stability |
| url | http://www.sciencedirect.com/science/article/pii/S2238785419310907 |
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