Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik
Aim: The present study was undertaken to evaluate the potential role of ethanol extract of seeds of Lens culinaris in attenuation of Cisplatin-induced nephrotoxicity in male Albino rats. Materials and Methods: Seeds of Lens culinaris were powdered, defatted and ethanol extract was prepared by hot ex...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Ancient Science of Life |
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| Online Access: | http://www.ancientscienceoflife.org/article.asp?issn=0257-7941;year=2017;volume=37;issue=2;spage=74;epage=80;aulast=Sreedevi |
| _version_ | 1798044920804540416 |
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| author | Adikay Sreedevi Kaveripakam Saisruthi |
| author_facet | Adikay Sreedevi Kaveripakam Saisruthi |
| author_sort | Adikay Sreedevi |
| collection | DOAJ |
| description | Aim: The present study was undertaken to evaluate the potential role of ethanol extract of seeds of Lens culinaris in attenuation of Cisplatin-induced nephrotoxicity in male Albino rats. Materials and Methods: Seeds of Lens culinaris were powdered, defatted and ethanol extract was prepared by hot extraction method. The ethanol extract so prepared was subjected to preliminary phytochemical evaluation and fingerprint analysis using high performance thin layer chromatography. Nephroprotective potential of extract was evaluated at 200 and 400 mg/kg b.w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intra peritoneal injection of Cisplatin at a dose of 5 mg/kg b.w. Nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels in renal tissue. Results: Cisplatin had induced marked acute renal toxicity as manifested by a significant increase in serum marker levels, urinary total protein, lipid peroxidation and decrease in urinary creatinine, catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH). The administration of extract significantly attenuated the Cisplatin-induced nephrotoxicity remarkably by restoring the biochemical and oxidative stress markers in both curative and prophylactic regimens in a dose dependent manner. Histological and immunohistochemical studies also substantiated the biochemical studies. Conclusion: The findings of the present study provides corroborative scientific evidence for the attenuation of Cisplatin-induced nephrotoxicity by seeds of Lens culinaris and validates the ethno-medicinal use of this seeds as renoprotective agent. |
| first_indexed | 2024-04-11T23:12:44Z |
| format | Article |
| id | doaj.art-08922a4b330140a7bc145cef2578f101 |
| institution | Directory Open Access Journal |
| issn | 0257-7941 |
| language | English |
| last_indexed | 2024-04-11T23:12:44Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Ancient Science of Life |
| spelling | doaj.art-08922a4b330140a7bc145cef2578f1012022-12-22T03:57:46ZengWolters Kluwer Medknow PublicationsAncient Science of Life0257-79412017-01-01372748010.4103/asl.ASL_173_17Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medikAdikay SreedeviKaveripakam SaisruthiAim: The present study was undertaken to evaluate the potential role of ethanol extract of seeds of Lens culinaris in attenuation of Cisplatin-induced nephrotoxicity in male Albino rats. Materials and Methods: Seeds of Lens culinaris were powdered, defatted and ethanol extract was prepared by hot extraction method. The ethanol extract so prepared was subjected to preliminary phytochemical evaluation and fingerprint analysis using high performance thin layer chromatography. Nephroprotective potential of extract was evaluated at 200 and 400 mg/kg b.w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intra peritoneal injection of Cisplatin at a dose of 5 mg/kg b.w. Nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels in renal tissue. Results: Cisplatin had induced marked acute renal toxicity as manifested by a significant increase in serum marker levels, urinary total protein, lipid peroxidation and decrease in urinary creatinine, catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH). The administration of extract significantly attenuated the Cisplatin-induced nephrotoxicity remarkably by restoring the biochemical and oxidative stress markers in both curative and prophylactic regimens in a dose dependent manner. Histological and immunohistochemical studies also substantiated the biochemical studies. Conclusion: The findings of the present study provides corroborative scientific evidence for the attenuation of Cisplatin-induced nephrotoxicity by seeds of Lens culinaris and validates the ethno-medicinal use of this seeds as renoprotective agent.http://www.ancientscienceoflife.org/article.asp?issn=0257-7941;year=2017;volume=37;issue=2;spage=74;epage=80;aulast=SreedeviCisplatinLens culinarisnephroprotectiveserum markers |
| spellingShingle | Adikay Sreedevi Kaveripakam Saisruthi Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik Ancient Science of Life Cisplatin Lens culinaris nephroprotective serum markers |
| title | Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik |
| title_full | Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik |
| title_fullStr | Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik |
| title_full_unstemmed | Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik |
| title_short | Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik |
| title_sort | attenuation of cisplatin induced nephrotoxicity by ethanol extract of seeds of lens culinaris medik |
| topic | Cisplatin Lens culinaris nephroprotective serum markers |
| url | http://www.ancientscienceoflife.org/article.asp?issn=0257-7941;year=2017;volume=37;issue=2;spage=74;epage=80;aulast=Sreedevi |
| work_keys_str_mv | AT adikaysreedevi attenuationofcisplatininducednephrotoxicitybyethanolextractofseedsoflensculinarismedik AT kaveripakamsaisruthi attenuationofcisplatininducednephrotoxicitybyethanolextractofseedsoflensculinarismedik |