The prevalence of Y chromosome microdeletions in Pakistani infertile men
Background: Microdeletions of the azoospermia factor locus of the long arm of Y chromosome are an etiological factor of severe oligozoospermia or azoospermia. Objective: The aim of this study was to investigate the prevalence of Y-chromosome microdeletions in AZF region and their role in infertility...
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Science, Yazd, Iran
2013-01-01
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| Series: | Iranian Journal of Reproductive Medicine |
| Subjects: | |
| Online Access: | http://www.ijrm.ir/index.php/ijrm/article/view/698 |
| _version_ | 1818136820053966848 |
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| author | Rubina Tabassum Siddiqui Nosheen Mujtaba Mamoona Naz |
| author_facet | Rubina Tabassum Siddiqui Nosheen Mujtaba Mamoona Naz |
| author_sort | Rubina Tabassum Siddiqui |
| collection | DOAJ |
| description | Background: Microdeletions of the azoospermia factor locus of the long arm of Y chromosome are an etiological factor of severe oligozoospermia or azoospermia. Objective: The aim of this study was to investigate the prevalence of Y-chromosome microdeletions in AZF region and their role in infertility in Pakistani population. Materials and Methods: The type of deletions in AZF locus were detected in infertile men (n=113) and the association of Y chromosome microdeletions with male infertility was assessed by including men (50) with normal karyotype and having children. Y chromosome microdeletions were detected by multiplex PCR using 10 sequence tagged sites namely sY81, sY130, sY141, sY142, sY155, sY157, sY160, sY182, sY231, and sY202 that covered all three regions of AZF. Results: Individuals with severe oligozoospermia showed 2.86% deletion frequency in AZFc region as compared to azoospermic males (5.5%). Conclusion: The results of our study showed that deletions in Y chromosome are not playing major part in male infertility. Moreover, multiplex-PCR strategy might preferably be employed for the detection of Y chromosome microdeletions allied to male infertility. |
| first_indexed | 2024-12-11T09:46:28Z |
| format | Article |
| id | doaj.art-0892bbf657cb4950ae475dc90b3991d3 |
| institution | Directory Open Access Journal |
| issn | 1680-6433 2008-2177 |
| language | English |
| last_indexed | 2024-12-11T09:46:28Z |
| publishDate | 2013-01-01 |
| publisher | Shahid Sadoughi University of Medical Science, Yazd, Iran |
| record_format | Article |
| series | Iranian Journal of Reproductive Medicine |
| spelling | doaj.art-0892bbf657cb4950ae475dc90b3991d32022-12-22T01:12:32ZengShahid Sadoughi University of Medical Science, Yazd, IranIranian Journal of Reproductive Medicine1680-64332008-21772013-01-01118619624The prevalence of Y chromosome microdeletions in Pakistani infertile menRubina Tabassum SiddiquiNosheen MujtabaMamoona NazBackground: Microdeletions of the azoospermia factor locus of the long arm of Y chromosome are an etiological factor of severe oligozoospermia or azoospermia. Objective: The aim of this study was to investigate the prevalence of Y-chromosome microdeletions in AZF region and their role in infertility in Pakistani population. Materials and Methods: The type of deletions in AZF locus were detected in infertile men (n=113) and the association of Y chromosome microdeletions with male infertility was assessed by including men (50) with normal karyotype and having children. Y chromosome microdeletions were detected by multiplex PCR using 10 sequence tagged sites namely sY81, sY130, sY141, sY142, sY155, sY157, sY160, sY182, sY231, and sY202 that covered all three regions of AZF. Results: Individuals with severe oligozoospermia showed 2.86% deletion frequency in AZFc region as compared to azoospermic males (5.5%). Conclusion: The results of our study showed that deletions in Y chromosome are not playing major part in male infertility. Moreover, multiplex-PCR strategy might preferably be employed for the detection of Y chromosome microdeletions allied to male infertility.http://www.ijrm.ir/index.php/ijrm/article/view/698Male infertilityAzoospermiaY Chromosome deletions |
| spellingShingle | Rubina Tabassum Siddiqui Nosheen Mujtaba Mamoona Naz The prevalence of Y chromosome microdeletions in Pakistani infertile men Iranian Journal of Reproductive Medicine Male infertility Azoospermia Y Chromosome deletions |
| title | The prevalence of Y chromosome microdeletions in Pakistani infertile men |
| title_full | The prevalence of Y chromosome microdeletions in Pakistani infertile men |
| title_fullStr | The prevalence of Y chromosome microdeletions in Pakistani infertile men |
| title_full_unstemmed | The prevalence of Y chromosome microdeletions in Pakistani infertile men |
| title_short | The prevalence of Y chromosome microdeletions in Pakistani infertile men |
| title_sort | prevalence of y chromosome microdeletions in pakistani infertile men |
| topic | Male infertility Azoospermia Y Chromosome deletions |
| url | http://www.ijrm.ir/index.php/ijrm/article/view/698 |
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