Serum nerve growth factor in horses with osteoarthritis‐associated lameness
Abstract Background Nerve growth factor (NGF) is a neurotrophin that is increased in osteoarthritic joints of horses. In humans, NGF has been associated with pain, and both synovial and serum NGF concentrations are increased in osteoarthritic patients. Studies in humans also have shown that serum NG...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16718 |
| _version_ | 1797816156608790528 |
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| author | Anna Kendall Claudia Lützelschwab Johan Lundblad Eva Skiöldebrand |
| author_facet | Anna Kendall Claudia Lützelschwab Johan Lundblad Eva Skiöldebrand |
| author_sort | Anna Kendall |
| collection | DOAJ |
| description | Abstract Background Nerve growth factor (NGF) is a neurotrophin that is increased in osteoarthritic joints of horses. In humans, NGF has been associated with pain, and both synovial and serum NGF concentrations are increased in osteoarthritic patients. Studies in humans also have shown that serum NGF concentration can increase with stress. Serum NGF concentration should be evaluated in horses with osteoarthritis‐associated lameness. Objectives Quantify and compare serum NGF concentration in horses with osteoarthritis‐associated lameness and sound horses. Additionally, the impact of short‐term stress on serum NGF concentration was investigated. Animals Lame horses with radiographic evidence of osteoarthritis (n = 20), lame horses without radiographic changes in the affected joint (n = 20) and sound horses (n = 20). In addition, horses with acute fractures (n = 9) were sampled. To determine the effect of stress, serum from horses subjected to a stressful event (transportation, n = 5; stress confirmed by increased serum cortisol concentration) was analyzed. Methods Cross‐sectional clinical study (lame, sound, and fracture cohorts) and experimental longitudinal study (stress cohort). Serum NGF concentration was determined using a quantitative sandwich ELISA. Results Serum NGF concentration was increased in lame horses with radiographic evidence of osteoarthritis (P < .0001; median, 238 pg/mL; interquartile range [IQR], 63‐945 pg/mL) and in lame horses without radiographic evidence of osteoarthritis in the painful joint (P < .05; median, 31 pg/mL; IQR, 31‐95 pg/mL) compared with sound horses (median, 31 pg/mL; IQR, 31‐46 pg/mL). Serum NGF concentration did not increase with short‐term stress and was low in horses with fracture‐associated pain. Conclusions and Clinical Importance Serum NGF concentration was high in the cohort with advanced osteoarthritis and should be investigated as a marker for osteoarthritis‐associated pain. |
| first_indexed | 2024-03-13T08:33:31Z |
| format | Article |
| id | doaj.art-089445d085e04e57b0eabdd7cfa602f2 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-03-13T08:33:31Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-089445d085e04e57b0eabdd7cfa602f22023-05-31T01:33:58ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762023-05-013731201120810.1111/jvim.16718Serum nerve growth factor in horses with osteoarthritis‐associated lamenessAnna Kendall0Claudia Lützelschwab1Johan Lundblad2Eva Skiöldebrand3Division of Pathology, Pharmacology and Toxicology, Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala SwedenDivision of Pathology, Pharmacology and Toxicology, Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala SwedenDepartment of Anatomy, Physiology and Biochemistry Swedish University of Agricultural Sciences Uppsala SwedenDivision of Pathology, Pharmacology and Toxicology, Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala SwedenAbstract Background Nerve growth factor (NGF) is a neurotrophin that is increased in osteoarthritic joints of horses. In humans, NGF has been associated with pain, and both synovial and serum NGF concentrations are increased in osteoarthritic patients. Studies in humans also have shown that serum NGF concentration can increase with stress. Serum NGF concentration should be evaluated in horses with osteoarthritis‐associated lameness. Objectives Quantify and compare serum NGF concentration in horses with osteoarthritis‐associated lameness and sound horses. Additionally, the impact of short‐term stress on serum NGF concentration was investigated. Animals Lame horses with radiographic evidence of osteoarthritis (n = 20), lame horses without radiographic changes in the affected joint (n = 20) and sound horses (n = 20). In addition, horses with acute fractures (n = 9) were sampled. To determine the effect of stress, serum from horses subjected to a stressful event (transportation, n = 5; stress confirmed by increased serum cortisol concentration) was analyzed. Methods Cross‐sectional clinical study (lame, sound, and fracture cohorts) and experimental longitudinal study (stress cohort). Serum NGF concentration was determined using a quantitative sandwich ELISA. Results Serum NGF concentration was increased in lame horses with radiographic evidence of osteoarthritis (P < .0001; median, 238 pg/mL; interquartile range [IQR], 63‐945 pg/mL) and in lame horses without radiographic evidence of osteoarthritis in the painful joint (P < .05; median, 31 pg/mL; IQR, 31‐95 pg/mL) compared with sound horses (median, 31 pg/mL; IQR, 31‐46 pg/mL). Serum NGF concentration did not increase with short‐term stress and was low in horses with fracture‐associated pain. Conclusions and Clinical Importance Serum NGF concentration was high in the cohort with advanced osteoarthritis and should be investigated as a marker for osteoarthritis‐associated pain.https://doi.org/10.1111/jvim.16718equineNGFosteoarthritispain |
| spellingShingle | Anna Kendall Claudia Lützelschwab Johan Lundblad Eva Skiöldebrand Serum nerve growth factor in horses with osteoarthritis‐associated lameness Journal of Veterinary Internal Medicine equine NGF osteoarthritis pain |
| title | Serum nerve growth factor in horses with osteoarthritis‐associated lameness |
| title_full | Serum nerve growth factor in horses with osteoarthritis‐associated lameness |
| title_fullStr | Serum nerve growth factor in horses with osteoarthritis‐associated lameness |
| title_full_unstemmed | Serum nerve growth factor in horses with osteoarthritis‐associated lameness |
| title_short | Serum nerve growth factor in horses with osteoarthritis‐associated lameness |
| title_sort | serum nerve growth factor in horses with osteoarthritis associated lameness |
| topic | equine NGF osteoarthritis pain |
| url | https://doi.org/10.1111/jvim.16718 |
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