An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies
An infrared (IR) absorbance sensor has been designed, realized and tested with the aim of detecting malignant melanomas in human skin biopsies. The sensor has been designed to obtain fast measurements (80 s) of a biopsy using a small light spot (0.5 mm in diameter, typically five to 10 times smaller...
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| Format: | Article |
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MDPI AG
2016-10-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/16/10/1659 |
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| author | Valeria Fioravanti Lukas Brandhoff Sander van den Driesche Heimo Breiteneder Melitta Kitzwögerer Christine Hafner Michael J. Vellekoop |
| author_facet | Valeria Fioravanti Lukas Brandhoff Sander van den Driesche Heimo Breiteneder Melitta Kitzwögerer Christine Hafner Michael J. Vellekoop |
| author_sort | Valeria Fioravanti |
| collection | DOAJ |
| description | An infrared (IR) absorbance sensor has been designed, realized and tested with the aim of detecting malignant melanomas in human skin biopsies. The sensor has been designed to obtain fast measurements (80 s) of a biopsy using a small light spot (0.5 mm in diameter, typically five to 10 times smaller than the biopsy size) to investigate different biopsy areas. The sensor has been equipped with a monochromator to record the whole IR spectrum in the 3330–3570 nm wavelength range (where methylene and methyl stretching vibrations occur) for a qualitative spectral investigation. From the collected spectra, the CH2 stretch ratio values (ratio of the absorption intensities of the symmetric to asymmetric CH2 stretching peaks) are determined and studied as a cancer indicator. Melanoma areas exhibit different spectral shapes and significantly higher CH2 stretch ratios when compared to healthy skin. The results of the infrared investigation are compared with standard histology. This study shows that the IR sensor is a promising supportive tool to improve the diagnosis of melanoma during histopathological analysis, decreasing the risk of misdiagnosis. |
| first_indexed | 2024-04-11T11:08:06Z |
| format | Article |
| id | doaj.art-08976aa0bea446c18b3821bff8c356b6 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T11:08:06Z |
| publishDate | 2016-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-08976aa0bea446c18b3821bff8c356b62022-12-22T04:28:14ZengMDPI AGSensors1424-82202016-10-011610165910.3390/s16101659s16101659An Infrared Absorbance Sensor for the Detection of Melanoma in Skin BiopsiesValeria Fioravanti0Lukas Brandhoff1Sander van den Driesche2Heimo Breiteneder3Melitta Kitzwögerer4Christine Hafner5Michael J. Vellekoop6Institute for Microsensors, Actuators and Systems (IMSAS), MCB, University of Bremen, Bremen D-28359, GermanyInstitute for Microsensors, Actuators and Systems (IMSAS), MCB, University of Bremen, Bremen D-28359, GermanyInstitute for Microsensors, Actuators and Systems (IMSAS), MCB, University of Bremen, Bremen D-28359, GermanyDepartment of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna A-1090, AustriaDepartment of Pathology, University Hospital St. Poelten, Karl Landsteiner University of Health Sciences, St. Poelten A-3100, AustriaDepartment of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna A-1090, AustriaInstitute for Microsensors, Actuators and Systems (IMSAS), MCB, University of Bremen, Bremen D-28359, GermanyAn infrared (IR) absorbance sensor has been designed, realized and tested with the aim of detecting malignant melanomas in human skin biopsies. The sensor has been designed to obtain fast measurements (80 s) of a biopsy using a small light spot (0.5 mm in diameter, typically five to 10 times smaller than the biopsy size) to investigate different biopsy areas. The sensor has been equipped with a monochromator to record the whole IR spectrum in the 3330–3570 nm wavelength range (where methylene and methyl stretching vibrations occur) for a qualitative spectral investigation. From the collected spectra, the CH2 stretch ratio values (ratio of the absorption intensities of the symmetric to asymmetric CH2 stretching peaks) are determined and studied as a cancer indicator. Melanoma areas exhibit different spectral shapes and significantly higher CH2 stretch ratios when compared to healthy skin. The results of the infrared investigation are compared with standard histology. This study shows that the IR sensor is a promising supportive tool to improve the diagnosis of melanoma during histopathological analysis, decreasing the risk of misdiagnosis.http://www.mdpi.com/1424-8220/16/10/1659infrared sensorabsorbance spectroscopyskin biopsymelanoma |
| spellingShingle | Valeria Fioravanti Lukas Brandhoff Sander van den Driesche Heimo Breiteneder Melitta Kitzwögerer Christine Hafner Michael J. Vellekoop An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies Sensors infrared sensor absorbance spectroscopy skin biopsy melanoma |
| title | An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies |
| title_full | An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies |
| title_fullStr | An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies |
| title_full_unstemmed | An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies |
| title_short | An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies |
| title_sort | infrared absorbance sensor for the detection of melanoma in skin biopsies |
| topic | infrared sensor absorbance spectroscopy skin biopsy melanoma |
| url | http://www.mdpi.com/1424-8220/16/10/1659 |
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