Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography
Three anthraquinones—emodin, chrysophanol, and physcion—were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane–ethanol–water (18:22:3, v/v/v) as the two-ph...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/16/2/1201/ |
| _version_ | 1811262825034678272 |
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| author | Shuying Guo Bo Feng Ruonan Zhu Jiankang Ma Wei Wang |
| author_facet | Shuying Guo Bo Feng Ruonan Zhu Jiankang Ma Wei Wang |
| author_sort | Shuying Guo |
| collection | DOAJ |
| description | Three anthraquinones—emodin, chrysophanol, and physcion—were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane–ethanol–water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by 1H-NMR and 13C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC. |
| first_indexed | 2024-04-12T19:32:36Z |
| format | Article |
| id | doaj.art-0898086e2afe4ebf8697d9e1d22ed30c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T19:32:36Z |
| publishDate | 2011-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0898086e2afe4ebf8697d9e1d22ed30c2022-12-22T03:19:17ZengMDPI AGMolecules1420-30492011-01-011621201121010.3390/molecules16021201Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current ChromatographyShuying GuoBo FengRuonan ZhuJiankang MaWei WangThree anthraquinones—emodin, chrysophanol, and physcion—were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane–ethanol–water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by 1H-NMR and 13C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.http://www.mdpi.com/1420-3049/16/2/1201/Rumex japonicus Houtt.anthraquinoneshigh-speed counter-current chromatography (HSCCC)emodinchrysophanolphyscion |
| spellingShingle | Shuying Guo Bo Feng Ruonan Zhu Jiankang Ma Wei Wang Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography Molecules Rumex japonicus Houtt. anthraquinones high-speed counter-current chromatography (HSCCC) emodin chrysophanol physcion |
| title | Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography |
| title_full | Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography |
| title_fullStr | Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography |
| title_full_unstemmed | Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography |
| title_short | Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography |
| title_sort | preparative isolation of three anthraquinones from rumex japonicus by high speed counter current chromatography |
| topic | Rumex japonicus Houtt. anthraquinones high-speed counter-current chromatography (HSCCC) emodin chrysophanol physcion |
| url | http://www.mdpi.com/1420-3049/16/2/1201/ |
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