Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana.
Mitogen-activated protein kinases (MAPKs) target a variety of protein substrates to regulate cellular signaling processes in eukaryotes. In plants, the number of identified MAPK substrates that control plant defense responses is still limited. Here, we generated transgenic Arabidopsis thaliana plant...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00554/full |
| _version_ | 1811256616405696512 |
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| author | Ines eLassowskat Christoph eBoettcher Christoph eBoettcher Lennart eEschen-Lippold Dierk eScheel Justin eLee |
| author_facet | Ines eLassowskat Christoph eBoettcher Christoph eBoettcher Lennart eEschen-Lippold Dierk eScheel Justin eLee |
| author_sort | Ines eLassowskat |
| collection | DOAJ |
| description | Mitogen-activated protein kinases (MAPKs) target a variety of protein substrates to regulate cellular signaling processes in eukaryotes. In plants, the number of identified MAPK substrates that control plant defense responses is still limited. Here, we generated transgenic Arabidopsis thaliana plants with an inducible system to simulate in vivo activation of two stress-activated MAPKs, MPK3 and MPK6. Metabolome analysis revealed that this artificial MPK3/6 activation (without any exposure to pathogens or other stresses) is sufficient to drive the production of major defense-related metabolites, including various camalexin, indole glucosinolate and agmatine derivatives. An accompanying (phospho)proteome analysis led to detection of hundreds of potential phosphoproteins downstream of MPK3/6 activation. Besides known MAPK substrates, many candidates on this list possess typical MAPK-targeted phosphosites and in many cases, the corresponding phosphopeptides were detected by mass spectrometry. Notably, several of these putative phosphoproteins have been reported to be associated with the biosynthesis of antimicrobial defense substances (e.g. WRKY transcription factors and proteins encoded by the genes from the PEN pathway required for penetration resistance to filamentous pathogens). Thus, this work provides an inventory of candidate phosphoproteins, including putative direct MAPK substrates, for future analysis of MAPK-mediated defense control. (Proteomics data are available with the identifier PXD001252 via ProteomeXchange, http://proteomecentral.proteomexchange.org). |
| first_indexed | 2024-04-12T17:43:00Z |
| format | Article |
| id | doaj.art-08993929ac1943258b1a3992e582b079 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T17:43:00Z |
| publishDate | 2014-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-08993929ac1943258b1a3992e582b0792022-12-22T03:22:44ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2014-10-01510.3389/fpls.2014.00554110384Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana.Ines eLassowskat0Christoph eBoettcher1Christoph eBoettcher2Lennart eEschen-Lippold3Dierk eScheel4Justin eLee5Leibniz Institute of Plant BiochemistryLeibniz Institute of Plant BiochemistryJulius Kuehn Institute, Federal Research Centre for Cultivated PlantsLeibniz Institute of Plant BiochemistryLeibniz Institute of Plant BiochemistryLeibniz Institute of Plant BiochemistryMitogen-activated protein kinases (MAPKs) target a variety of protein substrates to regulate cellular signaling processes in eukaryotes. In plants, the number of identified MAPK substrates that control plant defense responses is still limited. Here, we generated transgenic Arabidopsis thaliana plants with an inducible system to simulate in vivo activation of two stress-activated MAPKs, MPK3 and MPK6. Metabolome analysis revealed that this artificial MPK3/6 activation (without any exposure to pathogens or other stresses) is sufficient to drive the production of major defense-related metabolites, including various camalexin, indole glucosinolate and agmatine derivatives. An accompanying (phospho)proteome analysis led to detection of hundreds of potential phosphoproteins downstream of MPK3/6 activation. Besides known MAPK substrates, many candidates on this list possess typical MAPK-targeted phosphosites and in many cases, the corresponding phosphopeptides were detected by mass spectrometry. Notably, several of these putative phosphoproteins have been reported to be associated with the biosynthesis of antimicrobial defense substances (e.g. WRKY transcription factors and proteins encoded by the genes from the PEN pathway required for penetration resistance to filamentous pathogens). Thus, this work provides an inventory of candidate phosphoproteins, including putative direct MAPK substrates, for future analysis of MAPK-mediated defense control. (Proteomics data are available with the identifier PXD001252 via ProteomeXchange, http://proteomecentral.proteomexchange.org).http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00554/fullMetabolomicsPhosphorylationDefensephosphoproteomicsphytoalexinsMAPK substrates |
| spellingShingle | Ines eLassowskat Christoph eBoettcher Christoph eBoettcher Lennart eEschen-Lippold Dierk eScheel Justin eLee Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana. Frontiers in Plant Science Metabolomics Phosphorylation Defense phosphoproteomics phytoalexins MAPK substrates |
| title | Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana. |
| title_full | Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana. |
| title_fullStr | Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana. |
| title_full_unstemmed | Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana. |
| title_short | Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana. |
| title_sort | sustained mitogen activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in arabidopsis thaliana |
| topic | Metabolomics Phosphorylation Defense phosphoproteomics phytoalexins MAPK substrates |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00554/full |
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