EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO)
Worldwide, the scientific researches performed during the last years are focused on the determination of the negative effects caused by natural and antropogeneous chemical compounds on aquatic species; these species are more exposed to most pollutants than the land species, for the simple reason tha...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/1416 |
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| author | GABI DUMITRESCU LILIANA PETCULESCU-CIOCHINA S. VOIA A. GROZEA D. DRONCA IULIANA CRETESCU LILIANA BOCA |
| author_facet | GABI DUMITRESCU LILIANA PETCULESCU-CIOCHINA S. VOIA A. GROZEA D. DRONCA IULIANA CRETESCU LILIANA BOCA |
| author_sort | GABI DUMITRESCU |
| collection | DOAJ |
| description | Worldwide, the scientific researches performed during the last years are focused on
the determination of the negative effects caused by natural and antropogeneous
chemical compounds on aquatic species; these species are more exposed to most
pollutants than the land species, for the simple reason that the aquatic environment
is the last destination for most residues. Our research team proposed to test the toxic
effect caused by ethinylestradiol on embryo development in common carp (Cyprinus
carpio). Common carp embryos were purchased from the fish farm S.C. Acva Prod
S.R.L. Cefa, Bihor County these were obtained by artificial reproduction. After
taking and selection, the fecundated spawns were introduced in 10 Nunk culture
plates of 45 ml, where we introduced 40 ml water, too. We created 3 batches, with
two replications, namely: batch 1 – control, batch 2 – in water, we added
ethinylestradiol (EE2) in concentration of 1.5 ng L-1 and batch 3 – we added in water
a concentration of 7 ng L-1 EE2. During the incubation, the Nunk plates were kept in
breeding aquariums, at a temperature of 24°C. Successive to the supervision of
embryos in batch 3, 48 hours post-fecundation, we could observe evolution
stagnations, 70% of them being in the stage of 40 somites of the segmentation
period. At the same age, 100% of the control batch- embryos entered the stage of
advanced faringula, and in batch 2 all embryos were in the stage of incipient
faringula. 60-72 hours post-fecundation, all embryos in the batch 3 died, 90% in the
40 somite stage of the segmentation period and 10% in the stage of incipient
faringula. 85 hours post-fecundation, all embryos belonging to the control batch
were in the larva stage, while in batch 2, 90% were in the larva stage and 10% died
in the stage of advanced faringula. |
| first_indexed | 2024-04-24T11:34:47Z |
| format | Article |
| id | doaj.art-089979bc853f415f9e60d16a845c8659 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:34:47Z |
| publishDate | 2023-10-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-089979bc853f415f9e60d16a845c86592024-04-10T05:31:20ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-10-0142233331416EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO)GABI DUMITRESCU0LILIANA PETCULESCU-CIOCHINA1S. VOIA2A. GROZEA3D. DRONCA4IULIANA CRETESCU5LILIANA BOCA6Faculty of Animal Sciences and Biotechnologies, Timisoara, România Faculty of Animal Sciences and Biotechnologies, Timisoara, România Faculty of Animal Sciences and Biotechnologies, Timisoara, România Faculty of Animal Sciences and Biotechnologies, Timisoara, România Faculty of Animal Sciences and Biotechnologies, Timisoara, România Faculty of Animal Sciences and Biotechnologies, Timisoara, România Faculty of Animal Sciences and Biotechnologies, Timisoara, România Worldwide, the scientific researches performed during the last years are focused on the determination of the negative effects caused by natural and antropogeneous chemical compounds on aquatic species; these species are more exposed to most pollutants than the land species, for the simple reason that the aquatic environment is the last destination for most residues. Our research team proposed to test the toxic effect caused by ethinylestradiol on embryo development in common carp (Cyprinus carpio). Common carp embryos were purchased from the fish farm S.C. Acva Prod S.R.L. Cefa, Bihor County these were obtained by artificial reproduction. After taking and selection, the fecundated spawns were introduced in 10 Nunk culture plates of 45 ml, where we introduced 40 ml water, too. We created 3 batches, with two replications, namely: batch 1 – control, batch 2 – in water, we added ethinylestradiol (EE2) in concentration of 1.5 ng L-1 and batch 3 – we added in water a concentration of 7 ng L-1 EE2. During the incubation, the Nunk plates were kept in breeding aquariums, at a temperature of 24°C. Successive to the supervision of embryos in batch 3, 48 hours post-fecundation, we could observe evolution stagnations, 70% of them being in the stage of 40 somites of the segmentation period. At the same age, 100% of the control batch- embryos entered the stage of advanced faringula, and in batch 2 all embryos were in the stage of incipient faringula. 60-72 hours post-fecundation, all embryos in the batch 3 died, 90% in the 40 somite stage of the segmentation period and 10% in the stage of incipient faringula. 85 hours post-fecundation, all embryos belonging to the control batch were in the larva stage, while in batch 2, 90% were in the larva stage and 10% died in the stage of advanced faringula.https://spasb.ro/index.php/public_html/article/view/1416embryoethinylestradiolcyprinus carpiodevelopment |
| spellingShingle | GABI DUMITRESCU LILIANA PETCULESCU-CIOCHINA S. VOIA A. GROZEA D. DRONCA IULIANA CRETESCU LILIANA BOCA EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO) Scientific Papers Animal Science and Biotechnologies embryo ethinylestradiol cyprinus carpio development |
| title | EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO) |
| title_full | EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO) |
| title_fullStr | EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO) |
| title_full_unstemmed | EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO) |
| title_short | EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO) |
| title_sort | evaluation of ethinylestradiol ee2 effect on embryo development in common carp cyprinus carpio |
| topic | embryo ethinylestradiol cyprinus carpio development |
| url | https://spasb.ro/index.php/public_html/article/view/1416 |
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