Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes
The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls; 72 samples) and Czech Fleckvieh (4 bulls; 72 samples) semen froz...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2018-10-01
|
| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201810-0003_effect-of-thawing-method-on-bull-sperm-survival-in-ejaculates-frozen-in-4-ml-and-8-ml-volumes.php |
| _version_ | 1797899166326718464 |
|---|---|
| author | Zuzana Biniová Luděk Stádník Martina Doležalová Jaromír Ducháček |
| author_facet | Zuzana Biniová Luděk Stádník Martina Doležalová Jaromír Ducháček |
| author_sort | Zuzana Biniová |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls; 72 samples) and Czech Fleckvieh (4 bulls; 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0.25 and 0.5 ml), thawing using the slow protocol (P1) was the most appropriate (P < 0.05) for both observed volumes. There were found significantly higher MOT in the volume of 8 ml in both T0 and T30 and in the volume of 4 ml in T30 in samples thawed using P1 and P2. MOT in T0 was significantly affected by breed in samples frozen in 8 ml and in T30 in samples frozen in 4 and 8 ml. There were found no significant differences in INT in all reported volumes, however decrease of INT during cryopreservation was affected by breed. |
| first_indexed | 2024-04-10T08:24:37Z |
| format | Article |
| id | doaj.art-0899a69442424f74ad1d2b4131767b11 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:24:37Z |
| publishDate | 2018-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-0899a69442424f74ad1d2b4131767b112023-02-23T03:33:35ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092018-10-016310399407cjs-201810-0003Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumesZuzana Biniová0Luděk Stádník1Martina Doležalová2Jaromír Ducháček3Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicThe aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls; 72 samples) and Czech Fleckvieh (4 bulls; 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0.25 and 0.5 ml), thawing using the slow protocol (P1) was the most appropriate (P < 0.05) for both observed volumes. There were found significantly higher MOT in the volume of 8 ml in both T0 and T30 and in the volume of 4 ml in T30 in samples thawed using P1 and P2. MOT in T0 was significantly affected by breed in samples frozen in 8 ml and in T30 in samples frozen in 4 and 8 ml. There were found no significant differences in INT in all reported volumes, however decrease of INT during cryopreservation was affected by breed.https://cjas.agriculturejournals.cz/artkey/cjs-201810-0003_effect-of-thawing-method-on-bull-sperm-survival-in-ejaculates-frozen-in-4-ml-and-8-ml-volumes.phpbovine semenlarge volumessemen processing, sperm quality parameters |
| spellingShingle | Zuzana Biniová Luděk Stádník Martina Doležalová Jaromír Ducháček Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes Czech Journal of Animal Science bovine semen large volumes semen processing, sperm quality parameters |
| title | Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes |
| title_full | Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes |
| title_fullStr | Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes |
| title_full_unstemmed | Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes |
| title_short | Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes |
| title_sort | effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes |
| topic | bovine semen large volumes semen processing, sperm quality parameters |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201810-0003_effect-of-thawing-method-on-bull-sperm-survival-in-ejaculates-frozen-in-4-ml-and-8-ml-volumes.php |
| work_keys_str_mv | AT zuzanabiniova effectofthawingmethodonbullspermsurvivalinejaculatesfrozenin4mland8mlvolumes AT ludekstadnik effectofthawingmethodonbullspermsurvivalinejaculatesfrozenin4mland8mlvolumes AT martinadolezalova effectofthawingmethodonbullspermsurvivalinejaculatesfrozenin4mland8mlvolumes AT jaromirduchacek effectofthawingmethodonbullspermsurvivalinejaculatesfrozenin4mland8mlvolumes |