Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (<i>Birnaviridae</i>) Isolated from Mosquitoes Collected in East Texas, USA
This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of <i>Aedes sollicitans</i> mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that P...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/4/390 |
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| author | Robert B. Tesh Bethany G. Bolling Hilda Guzman Vsevolod L. Popov Ashley Wilson Steven G. Widen Thomas G. Wood Peter J. Walker Nikos Vasilakis |
| author_facet | Robert B. Tesh Bethany G. Bolling Hilda Guzman Vsevolod L. Popov Ashley Wilson Steven G. Widen Thomas G. Wood Peter J. Walker Nikos Vasilakis |
| author_sort | Robert B. Tesh |
| collection | DOAJ |
| description | This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of <i>Aedes sollicitans</i> mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus <i>Entomobirnavirus</i>, family <i>Birnaviridae</i>. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter. |
| first_indexed | 2024-03-10T20:45:33Z |
| format | Article |
| id | doaj.art-089cbd47f9ec4dd08ef69f9ee970b557 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:45:33Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-089cbd47f9ec4dd08ef69f9ee970b5572023-11-19T20:17:32ZengMDPI AGViruses1999-49152020-03-0112439010.3390/v12040390Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (<i>Birnaviridae</i>) Isolated from Mosquitoes Collected in East Texas, USARobert B. Tesh0Bethany G. Bolling1Hilda Guzman2Vsevolod L. Popov3Ashley Wilson4Steven G. Widen5Thomas G. Wood6Peter J. Walker7Nikos Vasilakis8Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USAWorld Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0609, USADepartment of Pathology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USADepartment of Pathology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USAGalveston County Mosquito Control, 5115 Highway 3, Dickinson, TX 77539, USADepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555, USADepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555, USASchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, AustraliaDepartment of Pathology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USAThis report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of <i>Aedes sollicitans</i> mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus <i>Entomobirnavirus</i>, family <i>Birnaviridae</i>. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.https://www.mdpi.com/1999-4915/12/4/390<i>Birnaviridae</i><i>Entomobirnavirus</i>Port Bolivar virusmosquito viruses |
| spellingShingle | Robert B. Tesh Bethany G. Bolling Hilda Guzman Vsevolod L. Popov Ashley Wilson Steven G. Widen Thomas G. Wood Peter J. Walker Nikos Vasilakis Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (<i>Birnaviridae</i>) Isolated from Mosquitoes Collected in East Texas, USA Viruses <i>Birnaviridae</i> <i>Entomobirnavirus</i> Port Bolivar virus mosquito viruses |
| title | Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (<i>Birnaviridae</i>) Isolated from Mosquitoes Collected in East Texas, USA |
| title_full | Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (<i>Birnaviridae</i>) Isolated from Mosquitoes Collected in East Texas, USA |
| title_fullStr | Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (<i>Birnaviridae</i>) Isolated from Mosquitoes Collected in East Texas, USA |
| title_full_unstemmed | Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (<i>Birnaviridae</i>) Isolated from Mosquitoes Collected in East Texas, USA |
| title_short | Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (<i>Birnaviridae</i>) Isolated from Mosquitoes Collected in East Texas, USA |
| title_sort | characterization of port bolivar virus a novel entomobirnavirus i birnaviridae i isolated from mosquitoes collected in east texas usa |
| topic | <i>Birnaviridae</i> <i>Entomobirnavirus</i> Port Bolivar virus mosquito viruses |
| url | https://www.mdpi.com/1999-4915/12/4/390 |
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