Phytochemical screening, in vitro and in vivo effects of an aqueous extract of the bark of Combretum glutinosum Perr ex DC. (Combretaceae) on gastrointestinal strongyles
Introduction: Plants remain an inexhaustible source of medicines for the treatment of various diseases. The aim of this study was to evaluate the anthelmintic activity of the aqueous extract of Combretum glutinosum bark on adult gastrointestinal strongyle worms. Methods: In vitro, the extract was co...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Phytomedicine Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031323000878 |
| _version_ | 1797627077139103744 |
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| author | Placide Mahougnan Toklo Kétomon Pierre Challaton Mahoudo Fidèle Assogba Gilles-Christ Amos Akakpo Aboudou Habirou Kifouly Goué Géorcelin Alowanou Sylvie Hounzangbe-Adote Eléonore Chikani Yayi Joachim Djimon Gbenou |
| author_facet | Placide Mahougnan Toklo Kétomon Pierre Challaton Mahoudo Fidèle Assogba Gilles-Christ Amos Akakpo Aboudou Habirou Kifouly Goué Géorcelin Alowanou Sylvie Hounzangbe-Adote Eléonore Chikani Yayi Joachim Djimon Gbenou |
| author_sort | Placide Mahougnan Toklo |
| collection | DOAJ |
| description | Introduction: Plants remain an inexhaustible source of medicines for the treatment of various diseases. The aim of this study was to evaluate the anthelmintic activity of the aqueous extract of Combretum glutinosum bark on adult gastrointestinal strongyle worms. Methods: In vitro, the extract was contacted with worms at various doses. In vivo, 25 sheep were divided into five batches of 5 animals, with one batch untreated, one batch treated with albendazole and three batches treated with the extract at three different doses. The body weight of the animals, the number of eggs per gram of faeces and haematological parameters were used to assess the efficacy of the extract. Results: In vitro, the extract appeared to inhibit the motility of adult Haemonchus contortus worms more than Trichostrongylus colubriformis (P < 0.05). In vivo, a significant reduction in the faecal excretion rate was observed in batches of animals treated with the drug and those treated with the extract, compared with the untreated control. Similarly, an increase in animal weight and an improvement in haematological parameters were observed in the treated batches after experimentation. The chemical profile of the extract revealed the presence of various chemical groups suspected of being responsible for its anthelmintic properties. Conclusion: Treatment at a dose of 100 mg/kg body weight appeared to be more active in reducing the number of eggs per gram of faeces and could therefore be recommended for the formulation of an improved traditional medicines with anthelmintic properties. |
| first_indexed | 2024-03-11T10:19:15Z |
| format | Article |
| id | doaj.art-089d37a9f8334037a5b74ed36cf19cd8 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-03-11T10:19:15Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-089d37a9f8334037a5b74ed36cf19cd82023-11-16T06:11:11ZengElsevierPhytomedicine Plus2667-03132023-11-0134100491Phytochemical screening, in vitro and in vivo effects of an aqueous extract of the bark of Combretum glutinosum Perr ex DC. (Combretaceae) on gastrointestinal strongylesPlacide Mahougnan Toklo0Kétomon Pierre Challaton1Mahoudo Fidèle Assogba2Gilles-Christ Amos Akakpo3Aboudou Habirou Kifouly4Goué Géorcelin Alowanou5Sylvie Hounzangbe-Adote6Eléonore Chikani Yayi7Joachim Djimon Gbenou8Laboratoire de Pharmacognosie et des Huiles Essentielles, Département de Chimie, Facultés des Sciences et Techniques, Université d'Abomey Calavi, 01 BP: 918 ISBA Cotonou, Benin; Laboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, 01 BP 526 Cotonou, Benin; Corresponding author at: Laboratoire de Pharmacognosie et des Huiles Essentielles, Département de Chimie, Facultés des Sciences et Techniques, Université d'Abomey Calavi, 01 BP: 918 ISBA Cotonou, Benin.Laboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, 01 BP 526 Cotonou, BeninLaboratoire de Pharmacognosie et des Huiles Essentielles, Département de Chimie, Facultés des Sciences et Techniques, Université d'Abomey Calavi, 01 BP: 918 ISBA Cotonou, BeninLaboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, 01 BP 526 Cotonou, BeninLaboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, 01 BP 526 Cotonou, BeninLaboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, 01 BP 526 Cotonou, BeninLaboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, 01 BP 526 Cotonou, BeninLaboratoire de Pharmacognosie et des Huiles Essentielles, Département de Chimie, Facultés des Sciences et Techniques, Université d'Abomey Calavi, 01 BP: 918 ISBA Cotonou, BeninLaboratoire de Pharmacognosie et des Huiles Essentielles, Département de Chimie, Facultés des Sciences et Techniques, Université d'Abomey Calavi, 01 BP: 918 ISBA Cotonou, Benin; Laboratoire KABA de Chimie et Applications, Université Nationale des Sciences, Technologies, Ingénierie et Mathématiques, BP: 486 Abomey Sogbo Aliho, Benin; Corresponding author.Introduction: Plants remain an inexhaustible source of medicines for the treatment of various diseases. The aim of this study was to evaluate the anthelmintic activity of the aqueous extract of Combretum glutinosum bark on adult gastrointestinal strongyle worms. Methods: In vitro, the extract was contacted with worms at various doses. In vivo, 25 sheep were divided into five batches of 5 animals, with one batch untreated, one batch treated with albendazole and three batches treated with the extract at three different doses. The body weight of the animals, the number of eggs per gram of faeces and haematological parameters were used to assess the efficacy of the extract. Results: In vitro, the extract appeared to inhibit the motility of adult Haemonchus contortus worms more than Trichostrongylus colubriformis (P < 0.05). In vivo, a significant reduction in the faecal excretion rate was observed in batches of animals treated with the drug and those treated with the extract, compared with the untreated control. Similarly, an increase in animal weight and an improvement in haematological parameters were observed in the treated batches after experimentation. The chemical profile of the extract revealed the presence of various chemical groups suspected of being responsible for its anthelmintic properties. Conclusion: Treatment at a dose of 100 mg/kg body weight appeared to be more active in reducing the number of eggs per gram of faeces and could therefore be recommended for the formulation of an improved traditional medicines with anthelmintic properties.http://www.sciencedirect.com/science/article/pii/S2667031323000878AnthelminticIn vitroIn vivoChemical profileCombretum glutinosum |
| spellingShingle | Placide Mahougnan Toklo Kétomon Pierre Challaton Mahoudo Fidèle Assogba Gilles-Christ Amos Akakpo Aboudou Habirou Kifouly Goué Géorcelin Alowanou Sylvie Hounzangbe-Adote Eléonore Chikani Yayi Joachim Djimon Gbenou Phytochemical screening, in vitro and in vivo effects of an aqueous extract of the bark of Combretum glutinosum Perr ex DC. (Combretaceae) on gastrointestinal strongyles Phytomedicine Plus Anthelmintic In vitro In vivo Chemical profile Combretum glutinosum |
| title | Phytochemical screening, in vitro and in vivo effects of an aqueous extract of the bark of Combretum glutinosum Perr ex DC. (Combretaceae) on gastrointestinal strongyles |
| title_full | Phytochemical screening, in vitro and in vivo effects of an aqueous extract of the bark of Combretum glutinosum Perr ex DC. (Combretaceae) on gastrointestinal strongyles |
| title_fullStr | Phytochemical screening, in vitro and in vivo effects of an aqueous extract of the bark of Combretum glutinosum Perr ex DC. (Combretaceae) on gastrointestinal strongyles |
| title_full_unstemmed | Phytochemical screening, in vitro and in vivo effects of an aqueous extract of the bark of Combretum glutinosum Perr ex DC. (Combretaceae) on gastrointestinal strongyles |
| title_short | Phytochemical screening, in vitro and in vivo effects of an aqueous extract of the bark of Combretum glutinosum Perr ex DC. (Combretaceae) on gastrointestinal strongyles |
| title_sort | phytochemical screening in vitro and in vivo effects of an aqueous extract of the bark of combretum glutinosum perr ex dc combretaceae on gastrointestinal strongyles |
| topic | Anthelmintic In vitro In vivo Chemical profile Combretum glutinosum |
| url | http://www.sciencedirect.com/science/article/pii/S2667031323000878 |
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