Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro
Background: Enterococcus faecalis commonly infects root canals by forming a biofilm. Extracts from neem leaves (Azadirachta indica A. Juss) have been shown to have antibacterial properties, indicating their potential in preventing or treating biofilm formation caused by bacteria. Purpose: This study...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2023-06-01
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| Series: | Dental Journal |
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| Online Access: | https://e-journal.unair.ac.id/MKG/article/view/37411 |
| _version_ | 1797820344882429952 |
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| author | Suhartono Suhartono Cut Soraya Putri Shabira |
| author_facet | Suhartono Suhartono Cut Soraya Putri Shabira |
| author_sort | Suhartono Suhartono |
| collection | DOAJ |
| description | Background: Enterococcus faecalis commonly infects root canals by forming a biofilm. Extracts from neem leaves (Azadirachta indica A. Juss) have been shown to have antibacterial properties, indicating their potential in preventing or treating biofilm formation caused by bacteria. Purpose: This study aims to investigate the phytochemical compounds present in neem leaves (Azadirachta indica A. Juss) and establish the concentration of ethanol-based neem leaf extract that can effectively inhibit the in vitro growth of Enterococcus faecalis biofilm. Methods: This study employed the maceration technique for extraction, gas chromatography mass spectroscopy for the analysis of plant chemicals, and a microtiter plate assay for measuring biofilm formation with treatment concentrations of 6.25%, 12.5%, 25%, 50%, and 75%, with a positive control of 0.2% chlorhexidine. Results: A phytochemical analysis revealed that the ethanol extract of neem leaves contained 22 different metabolites, mainly terpenoids and fatty acids. The extract demonstrated antibiofilm activity only at a concentration of 12.5% with an average biofilm inhibition of 36.85%. However, lower concentrations of 6.25%, 25%, 50%, and 75% had the opposite effect, promoting biofilm formation in Enterococcus faecalis. Conclusion: Phytochemical metabolite contained in the ethanolic extracts of neem leaves might contribute a promising agent in treating a biofilm-mediated root canal infection of Enterococcus faecalis. |
| first_indexed | 2024-03-13T09:38:01Z |
| format | Article |
| id | doaj.art-089f44d5936946b2bf5a339e1bc0ebc5 |
| institution | Directory Open Access Journal |
| issn | 1978-3728 2442-9740 |
| language | English |
| last_indexed | 2024-03-13T09:38:01Z |
| publishDate | 2023-06-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Dental Journal |
| spelling | doaj.art-089f44d5936946b2bf5a339e1bc0ebc52023-05-25T06:58:26ZengUniversitas AirlanggaDental Journal1978-37282442-97402023-06-015629810310.20473/j.djmkg.v56.i2.p98-10335480Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitroSuhartono Suhartono0https://orcid.org/0000-0002-1052-0443Cut Soraya1Putri Shabira2Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, IndonesiaFaculty of Dentistry, Universitas Syiah Kuala, Banda Aceh, IndonesiaFaculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, IndonesiaBackground: Enterococcus faecalis commonly infects root canals by forming a biofilm. Extracts from neem leaves (Azadirachta indica A. Juss) have been shown to have antibacterial properties, indicating their potential in preventing or treating biofilm formation caused by bacteria. Purpose: This study aims to investigate the phytochemical compounds present in neem leaves (Azadirachta indica A. Juss) and establish the concentration of ethanol-based neem leaf extract that can effectively inhibit the in vitro growth of Enterococcus faecalis biofilm. Methods: This study employed the maceration technique for extraction, gas chromatography mass spectroscopy for the analysis of plant chemicals, and a microtiter plate assay for measuring biofilm formation with treatment concentrations of 6.25%, 12.5%, 25%, 50%, and 75%, with a positive control of 0.2% chlorhexidine. Results: A phytochemical analysis revealed that the ethanol extract of neem leaves contained 22 different metabolites, mainly terpenoids and fatty acids. The extract demonstrated antibiofilm activity only at a concentration of 12.5% with an average biofilm inhibition of 36.85%. However, lower concentrations of 6.25%, 25%, 50%, and 75% had the opposite effect, promoting biofilm formation in Enterococcus faecalis. Conclusion: Phytochemical metabolite contained in the ethanolic extracts of neem leaves might contribute a promising agent in treating a biofilm-mediated root canal infection of Enterococcus faecalis.https://e-journal.unair.ac.id/MKG/article/view/37411antibiofilmazadirachta indica a. jussenterococcus faecalisethanol extract |
| spellingShingle | Suhartono Suhartono Cut Soraya Putri Shabira Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro Dental Journal antibiofilm azadirachta indica a. juss enterococcus faecalis ethanol extract |
| title | Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro |
| title_full | Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro |
| title_fullStr | Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro |
| title_full_unstemmed | Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro |
| title_short | Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro |
| title_sort | antibiofilm activity of neem leaf azadirachta indica a juss ethanolic extracts against enterococcus faecalis in vitro |
| topic | antibiofilm azadirachta indica a. juss enterococcus faecalis ethanol extract |
| url | https://e-journal.unair.ac.id/MKG/article/view/37411 |
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