scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles
Abstract The assessment of AgNPs toxicity in vitro and in vivo models are frequently conflicting and inaccurate. Nevertheless, single cell immunological responses in a heterogenous environment have received little attention. Therefore, in this study, we have performed in-depth analysis which clearly...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-024-02364-0 |
| _version_ | 1797246974145069056 |
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| author | Haribalan Perumalsamy Xiao Xiao Hyun-Yi Kim Tae-Hyun Yoon |
| author_facet | Haribalan Perumalsamy Xiao Xiao Hyun-Yi Kim Tae-Hyun Yoon |
| author_sort | Haribalan Perumalsamy |
| collection | DOAJ |
| description | Abstract The assessment of AgNPs toxicity in vitro and in vivo models are frequently conflicting and inaccurate. Nevertheless, single cell immunological responses in a heterogenous environment have received little attention. Therefore, in this study, we have performed in-depth analysis which clearly revealed cellular-metal ion association as well as specific immunological response. Our study didn’t show significant population differences in PMBC between control and AgNPs group implying no toxicological response. To confirm it further, deep profiling identified differences in subsets and differentially expressed genes (DEGs) of monocytes, B cells and T cells. Notably, monocyte subsets showed significant upregulation of metallothionein (MT) gene expression such as MT1G, MT1X, MT1E, MT1A, and MT1F. On the other hand, downregulation of pro-inflammatory genes such as IL1β and CCL3 in both CD16 + and CD16- monocyte subsets were observed. This result indicated that AgNPs association with monocyte subsets de-promoted inflammatory responsive genes suggesting no significant toxicity observed in AgNPs treated group. Other cell types such as B cells and T cells also showed negligible differences in their subsets suggesting no toxicity response. Further, AgNPs treated group showed upregulation of cell proliferation, ribosomal synthesis, downregulation of cytokine release, and T cell differentiation inhibition. Overall, our results conclude that treatment of AgNPs to PMBC cells didn’t display immunological related cytotoxicity response and thus motivate researchers to use them actively for biomedical applications. |
| first_indexed | 2024-04-24T19:51:19Z |
| format | Article |
| id | doaj.art-08a0462b3c74470cbf2c72e7ca17ff23 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-24T19:51:19Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-08a0462b3c74470cbf2c72e7ca17ff232024-03-24T12:32:44ZengBMCJournal of Nanobiotechnology1477-31552024-03-0122111310.1186/s12951-024-02364-0scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticlesHaribalan Perumalsamy0Xiao Xiao1Hyun-Yi Kim2Tae-Hyun Yoon3Center for Creative Convergence Education, Hanyang UniversityDepartment of Chemistry, College of Natural Sciences, Hanyang UniversityNGeneS IncInstitute of Next Generation Material Design, Hanyang UniversityAbstract The assessment of AgNPs toxicity in vitro and in vivo models are frequently conflicting and inaccurate. Nevertheless, single cell immunological responses in a heterogenous environment have received little attention. Therefore, in this study, we have performed in-depth analysis which clearly revealed cellular-metal ion association as well as specific immunological response. Our study didn’t show significant population differences in PMBC between control and AgNPs group implying no toxicological response. To confirm it further, deep profiling identified differences in subsets and differentially expressed genes (DEGs) of monocytes, B cells and T cells. Notably, monocyte subsets showed significant upregulation of metallothionein (MT) gene expression such as MT1G, MT1X, MT1E, MT1A, and MT1F. On the other hand, downregulation of pro-inflammatory genes such as IL1β and CCL3 in both CD16 + and CD16- monocyte subsets were observed. This result indicated that AgNPs association with monocyte subsets de-promoted inflammatory responsive genes suggesting no significant toxicity observed in AgNPs treated group. Other cell types such as B cells and T cells also showed negligible differences in their subsets suggesting no toxicity response. Further, AgNPs treated group showed upregulation of cell proliferation, ribosomal synthesis, downregulation of cytokine release, and T cell differentiation inhibition. Overall, our results conclude that treatment of AgNPs to PMBC cells didn’t display immunological related cytotoxicity response and thus motivate researchers to use them actively for biomedical applications.https://doi.org/10.1186/s12951-024-02364-0AgNPsscRNA-seq analysisToxicityCellular-metal ion associationMonocytes |
| spellingShingle | Haribalan Perumalsamy Xiao Xiao Hyun-Yi Kim Tae-Hyun Yoon scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles Journal of Nanobiotechnology AgNPs scRNA-seq analysis Toxicity Cellular-metal ion association Monocytes |
| title | scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles |
| title_full | scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles |
| title_fullStr | scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles |
| title_full_unstemmed | scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles |
| title_short | scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles |
| title_sort | scrna seq analysis discovered suppression of immunomodulatory dependent inflammatory response in pmbcs exposed to silver nanoparticles |
| topic | AgNPs scRNA-seq analysis Toxicity Cellular-metal ion association Monocytes |
| url | https://doi.org/10.1186/s12951-024-02364-0 |
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