Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats
Artificial sweeteners (AS) have been widely used as sugar substitutes to reduce calorie intake. However, it was reported that high doses of AS induced glucose intolerance via modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal mi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-12-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.787055/full |
| _version_ | 1798033611678547968 |
|---|---|
| author | Minchun Zhang Jie Chen Minglan Yang Cheng Qian Yu Liu Yicheng Qi Rilu Feng Mei Yang Wei Liu Jing Ma |
| author_facet | Minchun Zhang Jie Chen Minglan Yang Cheng Qian Yu Liu Yicheng Qi Rilu Feng Mei Yang Wei Liu Jing Ma |
| author_sort | Minchun Zhang |
| collection | DOAJ |
| description | Artificial sweeteners (AS) have been widely used as sugar substitutes to reduce calorie intake. However, it was reported that high doses of AS induced glucose intolerance via modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal microbiota in obesity. Eight weeks after high-fat diet (HFD), the male Sprague Dawley rats were randomly divided into four groups (6 in each group) and administrated by a daily gavage of 2 ml normal saline (CON), 0.54 mM sucralose (N054), 0.78 mM sucralose (N078), and 324 mM sucrose (S324), respectively. After 4 weeks, fecal samples were obtained and analyzed by 16S ribosomal RNA gene sequencing. The richness and diversity of fecal microbiota were not changed by sucralose or sucrose. Both 0.54 mM (0.43 mg) and 0.78 mM (0.62 mg) sucralose tended to reduce the beneficial bacteria, Lactobacillaceae and Akkermansiaceae. The relative abundance of family Acidaminoccaceae and its genus Phascolarctobacteriam were increased after 0.54 mM sucralose. In functional prediction, 0.54 mM sucralose increased profiles of carbohydrate metabolism, whereas 0.78 mM sucralose enhanced those of amino acid metabolism. The lower doses of sucralose might alter the compositions of fecal microbiota. The effects of sucralose in different dosages should be considered in the future study. |
| first_indexed | 2024-04-11T20:33:03Z |
| format | Article |
| id | doaj.art-08a12945d14e4c428e7a8f31115c8d63 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T20:33:03Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-08a12945d14e4c428e7a8f31115c8d632022-12-22T04:04:27ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-12-01810.3389/fnut.2021.787055787055Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese RatsMinchun ZhangJie ChenMinglan YangCheng QianYu LiuYicheng QiRilu FengMei YangWei LiuJing MaArtificial sweeteners (AS) have been widely used as sugar substitutes to reduce calorie intake. However, it was reported that high doses of AS induced glucose intolerance via modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal microbiota in obesity. Eight weeks after high-fat diet (HFD), the male Sprague Dawley rats were randomly divided into four groups (6 in each group) and administrated by a daily gavage of 2 ml normal saline (CON), 0.54 mM sucralose (N054), 0.78 mM sucralose (N078), and 324 mM sucrose (S324), respectively. After 4 weeks, fecal samples were obtained and analyzed by 16S ribosomal RNA gene sequencing. The richness and diversity of fecal microbiota were not changed by sucralose or sucrose. Both 0.54 mM (0.43 mg) and 0.78 mM (0.62 mg) sucralose tended to reduce the beneficial bacteria, Lactobacillaceae and Akkermansiaceae. The relative abundance of family Acidaminoccaceae and its genus Phascolarctobacteriam were increased after 0.54 mM sucralose. In functional prediction, 0.54 mM sucralose increased profiles of carbohydrate metabolism, whereas 0.78 mM sucralose enhanced those of amino acid metabolism. The lower doses of sucralose might alter the compositions of fecal microbiota. The effects of sucralose in different dosages should be considered in the future study.https://www.frontiersin.org/articles/10.3389/fnut.2021.787055/fullartificial sweetenerssucralosefecal microbiotaobesity16S ribosomal RNA gene analysis |
| spellingShingle | Minchun Zhang Jie Chen Minglan Yang Cheng Qian Yu Liu Yicheng Qi Rilu Feng Mei Yang Wei Liu Jing Ma Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats Frontiers in Nutrition artificial sweeteners sucralose fecal microbiota obesity 16S ribosomal RNA gene analysis |
| title | Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats |
| title_full | Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats |
| title_fullStr | Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats |
| title_full_unstemmed | Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats |
| title_short | Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats |
| title_sort | low doses of sucralose alter fecal microbiota in high fat diet induced obese rats |
| topic | artificial sweeteners sucralose fecal microbiota obesity 16S ribosomal RNA gene analysis |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.787055/full |
| work_keys_str_mv | AT minchunzhang lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT jiechen lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT minglanyang lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT chengqian lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT yuliu lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT yichengqi lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT rilufeng lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT meiyang lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT weiliu lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats AT jingma lowdosesofsucralosealterfecalmicrobiotainhighfatdietinducedobeserats |