Generation of motor neurons from human amygdala-derived neural stem-like cells
Objective(s): Among several cell sources, adult human neural stem/progenitor cells (hNS/PCs) have been considered outstanding cells for performing mechanistic studies in in vitro and in vivo models of neurological disorders as well as for potential utility in cell-based therapeutic approaches. Previ...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-11-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_11548_a7bde3b2eda8806114262f5bd4e373cf.pdf |
| _version_ | 1819139777083998208 |
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| author | Sedigheh Ghasemi Hadi Aligholi Pir Hossain Koulivand Maryam Jafaraian Hassan Hosseini Ravandi Maryam Khaleghi Ghadiri Ali Gorji |
| author_facet | Sedigheh Ghasemi Hadi Aligholi Pir Hossain Koulivand Maryam Jafaraian Hassan Hosseini Ravandi Maryam Khaleghi Ghadiri Ali Gorji |
| author_sort | Sedigheh Ghasemi |
| collection | DOAJ |
| description | Objective(s): Among several cell sources, adult human neural stem/progenitor cells (hNS/PCs) have been considered outstanding cells for performing mechanistic studies in in vitro and in vivo models of neurological disorders as well as for potential utility in cell-based therapeutic approaches. Previous studies addressed the isolation and culture of hNS/PCs from human neocortical and hippocampal tissues. However, little data are available on hNS/PCs obtained from the adult human amygdala.Materials and Methods: The present study explored the capacity of the amygdala harvested from resected brain tissues of patients with medically refractory epilepsy to generate neurosphere-like bodies and motor neuron-like cells.Results: Although the proliferation process was slow, a considerable amount of cells was obtained after the 3rd passage. In addition, the cells could generate motor neuron-like cells under appropriate culture conditions. Conclusion: Isolation and culture of these cells enable us to improve our knowledge of the role of the amygdala in some neurological and psychological disorders and provide a novel source for therapeutic cell transplantation. |
| first_indexed | 2024-12-22T11:28:03Z |
| format | Article |
| id | doaj.art-08a1e6cc296b4a278f01831e06b50a28 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-22T11:28:03Z |
| publishDate | 2018-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-08a1e6cc296b4a278f01831e06b50a282022-12-21T18:27:42ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-11-0121111155116010.22038/ijbms.2018.29587.714611548Generation of motor neurons from human amygdala-derived neural stem-like cellsSedigheh Ghasemi0Hadi Aligholi1Pir Hossain Koulivand2Maryam Jafaraian3Hassan Hosseini Ravandi4Maryam Khaleghi Ghadiri5Ali Gorji6Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, IranDepartment of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, IranShefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, IranShefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, IranShefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, IranDepartment of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, GermanyShefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran|Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany|Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Neurology, Westfälische Wilhelms-Universität Münster, Münster, Germany|Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Münster, GermanyObjective(s): Among several cell sources, adult human neural stem/progenitor cells (hNS/PCs) have been considered outstanding cells for performing mechanistic studies in in vitro and in vivo models of neurological disorders as well as for potential utility in cell-based therapeutic approaches. Previous studies addressed the isolation and culture of hNS/PCs from human neocortical and hippocampal tissues. However, little data are available on hNS/PCs obtained from the adult human amygdala.Materials and Methods: The present study explored the capacity of the amygdala harvested from resected brain tissues of patients with medically refractory epilepsy to generate neurosphere-like bodies and motor neuron-like cells.Results: Although the proliferation process was slow, a considerable amount of cells was obtained after the 3rd passage. In addition, the cells could generate motor neuron-like cells under appropriate culture conditions. Conclusion: Isolation and culture of these cells enable us to improve our knowledge of the role of the amygdala in some neurological and psychological disorders and provide a novel source for therapeutic cell transplantation.http://ijbms.mums.ac.ir/article_11548_a7bde3b2eda8806114262f5bd4e373cf.pdfBrainHippocampusIntractable epilepsyMotor NeuronNeural stem cells |
| spellingShingle | Sedigheh Ghasemi Hadi Aligholi Pir Hossain Koulivand Maryam Jafaraian Hassan Hosseini Ravandi Maryam Khaleghi Ghadiri Ali Gorji Generation of motor neurons from human amygdala-derived neural stem-like cells Iranian Journal of Basic Medical Sciences Brain Hippocampus Intractable epilepsy Motor Neuron Neural stem cells |
| title | Generation of motor neurons from human amygdala-derived neural stem-like cells |
| title_full | Generation of motor neurons from human amygdala-derived neural stem-like cells |
| title_fullStr | Generation of motor neurons from human amygdala-derived neural stem-like cells |
| title_full_unstemmed | Generation of motor neurons from human amygdala-derived neural stem-like cells |
| title_short | Generation of motor neurons from human amygdala-derived neural stem-like cells |
| title_sort | generation of motor neurons from human amygdala derived neural stem like cells |
| topic | Brain Hippocampus Intractable epilepsy Motor Neuron Neural stem cells |
| url | http://ijbms.mums.ac.ir/article_11548_a7bde3b2eda8806114262f5bd4e373cf.pdf |
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