The expression of Ldh-c in the skeletal muscle of plateau pika (Ochotona curzoniae) enhances adaptation to a hypoxic environment
The plateau pika (Ochotona curzoniae) is a species of sprint-running alpine animals in the Qinghai-Tibet Plateau, which is a harsh highland hypoxic environment. Ldh-c is expressed in the testis, sperm and somatic tissues of plateau pika. To reveal the role and physiological mechanisms of sperm-speci...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2017-09-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/6/9/1336 |
| _version_ | 1818740222747213824 |
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| author | Zhi F. An Deng B. Wei Lian Wei Yang Wang Lin N. Wei |
| author_facet | Zhi F. An Deng B. Wei Lian Wei Yang Wang Lin N. Wei |
| author_sort | Zhi F. An |
| collection | DOAJ |
| description | The plateau pika (Ochotona curzoniae) is a species of sprint-running alpine animals in the Qinghai-Tibet Plateau, which is a harsh highland hypoxic environment. Ldh-c is expressed in the testis, sperm and somatic tissues of plateau pika. To reveal the role and physiological mechanisms of sperm-specific lactate dehydrogenase (LDH-C4), in plateau pika to adapt to hypoxic environment, an adenoviral line of pMultiRNAi-Ldhc was constructed and injected into the bilateral biceps femoris of the hind legs. The swimming times of the pikas, and the Ldh-c expression levels, total LDH activities and ATP levels in skeletal muscle, were measured after the pikas were raised in the trapped site for 5 days. Our results showed that after Ldh-c was silenced, the sprint-running ability (swimming time) of the plateau pikas was significant decreased, and the total LDH activities and ATP levels were reduced by 28.21% and 27.88%, respectively. Our results indicated that expression of Ldh-c in the skeletal muscle of plateau pika increased anaerobic glycolysis and enhanced adaptation to highland hypoxic environments. |
| first_indexed | 2024-12-18T01:37:18Z |
| format | Article |
| id | doaj.art-08a1f99b837a4184915a50229abfb20b |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-18T01:37:18Z |
| publishDate | 2017-09-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-08a1f99b837a4184915a50229abfb20b2022-12-21T21:25:25ZengThe Company of BiologistsBiology Open2046-63902017-09-01691336134110.1242/bio.024943024943The expression of Ldh-c in the skeletal muscle of plateau pika (Ochotona curzoniae) enhances adaptation to a hypoxic environmentZhi F. An0Deng B. Wei1Lian Wei2Yang Wang3Lin N. Wei4 State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University 10743, Xining, Qinghai 810016, China State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University 10743, Xining, Qinghai 810016, China College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai 810016, China Research Center for High Altitude Medicine, Qinghai University, Xining, Qinghai 810016, China College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai 810016, China The plateau pika (Ochotona curzoniae) is a species of sprint-running alpine animals in the Qinghai-Tibet Plateau, which is a harsh highland hypoxic environment. Ldh-c is expressed in the testis, sperm and somatic tissues of plateau pika. To reveal the role and physiological mechanisms of sperm-specific lactate dehydrogenase (LDH-C4), in plateau pika to adapt to hypoxic environment, an adenoviral line of pMultiRNAi-Ldhc was constructed and injected into the bilateral biceps femoris of the hind legs. The swimming times of the pikas, and the Ldh-c expression levels, total LDH activities and ATP levels in skeletal muscle, were measured after the pikas were raised in the trapped site for 5 days. Our results showed that after Ldh-c was silenced, the sprint-running ability (swimming time) of the plateau pikas was significant decreased, and the total LDH activities and ATP levels were reduced by 28.21% and 27.88%, respectively. Our results indicated that expression of Ldh-c in the skeletal muscle of plateau pika increased anaerobic glycolysis and enhanced adaptation to highland hypoxic environments.http://bio.biologists.org/content/6/9/1336Ldh-cPlateau pika (Ochotona curzoniae)HypoxiaQinghai-Tibet PlateauSkeletal muscle |
| spellingShingle | Zhi F. An Deng B. Wei Lian Wei Yang Wang Lin N. Wei The expression of Ldh-c in the skeletal muscle of plateau pika (Ochotona curzoniae) enhances adaptation to a hypoxic environment Biology Open Ldh-c Plateau pika (Ochotona curzoniae) Hypoxia Qinghai-Tibet Plateau Skeletal muscle |
| title | The expression of Ldh-c in the skeletal muscle of plateau pika (Ochotona curzoniae) enhances adaptation to a hypoxic environment |
| title_full | The expression of Ldh-c in the skeletal muscle of plateau pika (Ochotona curzoniae) enhances adaptation to a hypoxic environment |
| title_fullStr | The expression of Ldh-c in the skeletal muscle of plateau pika (Ochotona curzoniae) enhances adaptation to a hypoxic environment |
| title_full_unstemmed | The expression of Ldh-c in the skeletal muscle of plateau pika (Ochotona curzoniae) enhances adaptation to a hypoxic environment |
| title_short | The expression of Ldh-c in the skeletal muscle of plateau pika (Ochotona curzoniae) enhances adaptation to a hypoxic environment |
| title_sort | expression of ldh c in the skeletal muscle of plateau pika ochotona curzoniae enhances adaptation to a hypoxic environment |
| topic | Ldh-c Plateau pika (Ochotona curzoniae) Hypoxia Qinghai-Tibet Plateau Skeletal muscle |
| url | http://bio.biologists.org/content/6/9/1336 |
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