Screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewater
The characteristics of palm oil mill wastewater (POMW) were brown color, pH 3.8-4.3, temperature 48-55oC, total solids 68.2-82.1 g/l, suspended solids 26.2-65.6 g/l, oil and grease 19.1-25.1 g/l, COD 49.9-160.7g/l and BOD 32.5-75.3 g/l. After centrifugation (3,184 xg) of 50 ml POMW for 10 min, the P...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2007-05-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/29-3_online/0125-3395-29-3-0801-0808.pdf |
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| author | Aran H-Kittikun Treetippa Laohaprapanon Poonsuk Prasertsan |
| author_facet | Aran H-Kittikun Treetippa Laohaprapanon Poonsuk Prasertsan |
| author_sort | Aran H-Kittikun |
| collection | DOAJ |
| description | The characteristics of palm oil mill wastewater (POMW) were brown color, pH 3.8-4.3, temperature 48-55oC, total solids 68.2-82.1 g/l, suspended solids 26.2-65.6 g/l, oil and grease 19.1-25.1 g/l, COD 49.9-160.7g/l and BOD 32.5-75.3 g/l. After centrifugation (3,184 xg) of 50 ml POMW for 10 min, the POMW was separated into 3 layers: top (oil), middle (supernatant) and bottom layer (sediment). The sediment containeddry weight 1.19 g and oil and grease 1.07 g. In order to release oil and grease trapped in palm fiber debris in the POMW, cellulase- and/or xylanase-enzyme-producing and thermotolerant microorganisms wereisolated. The isolates SO1 and SO2 were isolated from soil near the first anaerobic pond of the palm oil mill. They were aerobic, Gram positive, rod shaped, thermotolerant microorganisms and produced cellulase 12.11 U/ml (3 days) and 7.2 U/ml (4 days), and xylanase 50.98 U/ml (4 days) and 20.42 U/ml (4 days), respectivelyin synthetic medium containing carboxymethycellulose as a carbon source. When these 2 isolates were added into the steriled POMW under shaking condition for 7 days, after centrifugation at 3,184 xg the isolate SO1gave the better % reduction of dry weight (64.66 %) and of oil and grease in the bottom layer (85.32 %) of the POMW. |
| first_indexed | 2024-12-13T19:22:51Z |
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| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-13T19:22:51Z |
| publishDate | 2007-05-01 |
| publisher | Prince of Songkla University |
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| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-08a55616237e4246b4f1ae96cffda95d2022-12-21T23:34:07ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952007-05-01293801808Screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewaterAran H-KittikunTreetippa LaohaprapanonPoonsuk PrasertsanThe characteristics of palm oil mill wastewater (POMW) were brown color, pH 3.8-4.3, temperature 48-55oC, total solids 68.2-82.1 g/l, suspended solids 26.2-65.6 g/l, oil and grease 19.1-25.1 g/l, COD 49.9-160.7g/l and BOD 32.5-75.3 g/l. After centrifugation (3,184 xg) of 50 ml POMW for 10 min, the POMW was separated into 3 layers: top (oil), middle (supernatant) and bottom layer (sediment). The sediment containeddry weight 1.19 g and oil and grease 1.07 g. In order to release oil and grease trapped in palm fiber debris in the POMW, cellulase- and/or xylanase-enzyme-producing and thermotolerant microorganisms wereisolated. The isolates SO1 and SO2 were isolated from soil near the first anaerobic pond of the palm oil mill. They were aerobic, Gram positive, rod shaped, thermotolerant microorganisms and produced cellulase 12.11 U/ml (3 days) and 7.2 U/ml (4 days), and xylanase 50.98 U/ml (4 days) and 20.42 U/ml (4 days), respectivelyin synthetic medium containing carboxymethycellulose as a carbon source. When these 2 isolates were added into the steriled POMW under shaking condition for 7 days, after centrifugation at 3,184 xg the isolate SO1gave the better % reduction of dry weight (64.66 %) and of oil and grease in the bottom layer (85.32 %) of the POMW.http://www.sjst.psu.ac.th/journal/29-3_online/0125-3395-29-3-0801-0808.pdfPalm oil mill wastewater (POMW)oil separationthermotolerant microorganismscellulasexylanase |
| spellingShingle | Aran H-Kittikun Treetippa Laohaprapanon Poonsuk Prasertsan Screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewater Songklanakarin Journal of Science and Technology (SJST) Palm oil mill wastewater (POMW) oil separation thermotolerant microorganisms cellulase xylanase |
| title | Screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewater |
| title_full | Screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewater |
| title_fullStr | Screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewater |
| title_full_unstemmed | Screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewater |
| title_short | Screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewater |
| title_sort | screening of thermotolerant microorganisms and application for oil separation from palm oil mill wastewater |
| topic | Palm oil mill wastewater (POMW) oil separation thermotolerant microorganisms cellulase xylanase |
| url | http://www.sjst.psu.ac.th/journal/29-3_online/0125-3395-29-3-0801-0808.pdf |
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