Nucleic Acid Test versus Enzyme Linked Immunosorbent Assay for Screening of Human Immunodeficiency Virus in Donated Blood Units: A Comparative Study at a Blood Centre in Western Rajasthan, India
Introduction: With a population of 1.3 billion people and a frequency of Human Immunodeficiency Virus (HIV) (0.17-0.29%), Hepatitis B Virus (HBV) (3-4%), and Hepatitis C Virus (HCV) (0.09-15%) in the general population, maintaining blood safety is a difficult undertaking in India. The fourth generat...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2022-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/16846/57260_F(KM)_PF1(AG_OM)_PN(KM).pdf |
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| author | Rishi Mathur NL Mahawar DR Arya Arun Bharti |
| author_facet | Rishi Mathur NL Mahawar DR Arya Arun Bharti |
| author_sort | Rishi Mathur |
| collection | DOAJ |
| description | Introduction: With a population of 1.3 billion people and a frequency of Human Immunodeficiency Virus (HIV) (0.17-0.29%), Hepatitis B Virus (HBV) (3-4%), and Hepatitis C Virus (HCV) (0.09-15%) in the general population, maintaining blood safety is a difficult undertaking in India. The fourth generation Enzyme Linked Immunosorbent Assay (ELISA), which can be used in place of Nucleic Acid Amplification Test (NAT) testing in situations with limited resources, has been recommended as the minimal HIV test to increase the safety of blood transfusions.
Aim: To compare the fourth generation ELISA with NAT in the screening of transfusion transmissible HIV infection in blood donors.
Materials and Methods: A cross-sectional study was carried out at the Blood Centre, Department of Immunohaematology and Transfusion Medicine, Sardar Patel Medical College and Associated Groups of Hospitals, in Bikaner, Rajasthan, India. A total of 2000 voluntary and replacement blood donors were recruited consecutively between January 2020 to December 2021 (two years), and their samples were screened using fourth generation ELISA. All of these samples were sent to AIIMS in Jodhpur for NAT to identify HIV RNA, HCV RNA and HBV DNA.
Results: In this study, when the fourth generation ELISA negative samples were subjected to NAT, no sample was found to be reactive for HIV in NAT, i.e. there was no NAT yield for HIV.
Conclusion: As a bare minimum, the fourth generation ELISA test should be used for blood donor screening and can be considered a cost-effective and reliable test in a resource limited setting. However, additional tests can be advocated for an additional layer of blood safety. |
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| format | Article |
| id | doaj.art-08a9519f33e94eec8441b9604bcd96c9 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-10T19:51:17Z |
| publishDate | 2022-09-01 |
| publisher | JCDR Research and Publications Private Limited |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-08a9519f33e94eec8441b9604bcd96c92023-01-28T04:54:19ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2022-09-01169EC16EC2110.7860/JCDR/2022/57260.16846Nucleic Acid Test versus Enzyme Linked Immunosorbent Assay for Screening of Human Immunodeficiency Virus in Donated Blood Units: A Comparative Study at a Blood Centre in Western Rajasthan, IndiaRishi Mathur0NL Mahawar1DR Arya2Arun Bharti3Resident, Department of Immnohaematology and Transfusion Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.Professor, Department of Immnohaematology and Transfusion Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.Senior Professor, Department of Immnohaematology and Transfusion Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.Associate Professor, Department of Immnohaematology and Transfusion Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.Introduction: With a population of 1.3 billion people and a frequency of Human Immunodeficiency Virus (HIV) (0.17-0.29%), Hepatitis B Virus (HBV) (3-4%), and Hepatitis C Virus (HCV) (0.09-15%) in the general population, maintaining blood safety is a difficult undertaking in India. The fourth generation Enzyme Linked Immunosorbent Assay (ELISA), which can be used in place of Nucleic Acid Amplification Test (NAT) testing in situations with limited resources, has been recommended as the minimal HIV test to increase the safety of blood transfusions. Aim: To compare the fourth generation ELISA with NAT in the screening of transfusion transmissible HIV infection in blood donors. Materials and Methods: A cross-sectional study was carried out at the Blood Centre, Department of Immunohaematology and Transfusion Medicine, Sardar Patel Medical College and Associated Groups of Hospitals, in Bikaner, Rajasthan, India. A total of 2000 voluntary and replacement blood donors were recruited consecutively between January 2020 to December 2021 (two years), and their samples were screened using fourth generation ELISA. All of these samples were sent to AIIMS in Jodhpur for NAT to identify HIV RNA, HCV RNA and HBV DNA. Results: In this study, when the fourth generation ELISA negative samples were subjected to NAT, no sample was found to be reactive for HIV in NAT, i.e. there was no NAT yield for HIV. Conclusion: As a bare minimum, the fourth generation ELISA test should be used for blood donor screening and can be considered a cost-effective and reliable test in a resource limited setting. However, additional tests can be advocated for an additional layer of blood safety.https://www.jcdr.net/articles/PDF/16846/57260_F(KM)_PF1(AG_OM)_PN(KM).pdfamplification testblood donorssafety |
| spellingShingle | Rishi Mathur NL Mahawar DR Arya Arun Bharti Nucleic Acid Test versus Enzyme Linked Immunosorbent Assay for Screening of Human Immunodeficiency Virus in Donated Blood Units: A Comparative Study at a Blood Centre in Western Rajasthan, India Journal of Clinical and Diagnostic Research amplification test blood donors safety |
| title | Nucleic Acid Test versus Enzyme Linked Immunosorbent Assay for Screening of Human Immunodeficiency Virus in Donated Blood Units: A Comparative Study at a Blood Centre in Western Rajasthan, India |
| title_full | Nucleic Acid Test versus Enzyme Linked Immunosorbent Assay for Screening of Human Immunodeficiency Virus in Donated Blood Units: A Comparative Study at a Blood Centre in Western Rajasthan, India |
| title_fullStr | Nucleic Acid Test versus Enzyme Linked Immunosorbent Assay for Screening of Human Immunodeficiency Virus in Donated Blood Units: A Comparative Study at a Blood Centre in Western Rajasthan, India |
| title_full_unstemmed | Nucleic Acid Test versus Enzyme Linked Immunosorbent Assay for Screening of Human Immunodeficiency Virus in Donated Blood Units: A Comparative Study at a Blood Centre in Western Rajasthan, India |
| title_short | Nucleic Acid Test versus Enzyme Linked Immunosorbent Assay for Screening of Human Immunodeficiency Virus in Donated Blood Units: A Comparative Study at a Blood Centre in Western Rajasthan, India |
| title_sort | nucleic acid test versus enzyme linked immunosorbent assay for screening of human immunodeficiency virus in donated blood units a comparative study at a blood centre in western rajasthan india |
| topic | amplification test blood donors safety |
| url | https://www.jcdr.net/articles/PDF/16846/57260_F(KM)_PF1(AG_OM)_PN(KM).pdf |
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